천룡(天龍)의 항암효과에 대한 고찰

안태규,손창규,정태영,유화승,조정효,Ahn, Tae-Kyu,Son, Chang-Gue,Jeong, Tae-Yong,Yoo, Hwa-Seung,Cho, Jung-Hyo 대한암한의학회 2009 大韓癌韓醫學會誌 Vol.14 No.1

Gekko has been used for several diseases including cancer in Oriental medicine and fork herbalogy. Nevertheless, its origin as herbal medicine and its efficacy and mechanism as anti-tumor drug have not yet been thoroughly reported in Korea. This study aimed to investigate anti-tumor effect of Gekko through selected articles from cqvip database in China. In vitro and In vivo, Gekko could obviously inhibit tumor growth, induce tumor cells apoptosis, reduce micro-vessel density in tumor tissue through down regulating VEGF & bFGF protein expression, promote cytotoxicity of lymphocyte. Gekko could improve survival rate, relive clinical symptoms, improve quality of life, and relieve anti-tumor treatment reaction, suggesting that Gekko might be a effective anti-tumor drug.

식중독 균주에 대한 계피 추출물과 상용항생제의 항균 활성 비교 연구

안태규(Ahn, Tae-Kyu),조세희(Cho, Sehee),고준석(Go, Junseok),김동희(Kim, Donghui),박주연(Park, Juyeon),최동진(Choi, Dongjin),최민준(Choi, Minjun),김지인(Kim, Jee-In),임재환(Lim, Jae-Hwan) 한국과학영재교육학회 2019 과학영재교육 Vol.11 No.1

최근 우리나라 전통의학인 한약재를 중심으로 식물 추출물의 인체에 대한 다양한 생리 효능을 찾고자 하는 연구가 많이 진행되고 있다. 하지만 항생 화학물질과 천연항균 물질에 대한 효능 비교를 통한 대체 가능성을 모색한 연구는 부족하다. 따라서 본 실험에서는 식중독 유발 미생물 등에 항균 활성을 보이는 한약재 3종과 일상 주변의 식중독 균 5종, 그리고 시중에 판매되는 4종류의 상용 항생제를 사용하여 한약재 추출물와의 항균 활성을 비교 분석하였다. 양성 대조군으로 0.1mg/ml ampicillin과 음성대조군으로 증류수와 에탄올를 사용한 디스크 확산법을 수행하여 아래 3가지 결과를 제시하고자 한다. 첫째, 시중에서 판매하는 4종의 상용 항생제 중 B 제재 (DH사)의 실험에 사용한 5균주에 대하여 항균 효능이 가장 높게 나타났으며, 둘 째, 계피 에탄올 추출물의 항균 효능이 그람음성균과 그람양성균에 대하여 폭넓게 나타났다. 구체적으로 100,000 ppm 처리 조건에서 상용 항생제별로 양성대조군 대비 약 75~153% 범위의 항균 활성을 나타내는 것으로 확인하였다. 셋째로 상용 항생제 및 계피 추출물의 항균 활성은 그람 음성 세균과 그람 양성 세균에 대해 일부 차이를 나타나는 것을 확인하였다. 즉, 식중 독 균주인 E. coli 를 포함하는 그람음성균에서 계피에탄올 추출물은 대조군과 비교하여 133~153% 의 높은 항균활성을 나타냈다. 또한 그람양성균에 대해서는 75~122% 의 항균활성을 나타냈다. 결과적으로 상용 항생제들과의 항균 활성 능력 비교를 통하여 항생물질의 대체제로서 한약재의 활용 가능성을 제시하고자 한다. So far, many studies have been performed to find out the various physiological effects of plant extracts on the human, centering on traditional medicinal herbs of Korea. However, few researches are published for alternative possibilities by comparing the efficacy of antibiotics and herbal medicines. Therefore, in this study, the antibacterial activity of the herbal medicines extracts were compared and analyzed by using three kinds of medicinal herbs which have antibacterial activity, five kinds of food poisoning bacteria, and four kinds of commercial antibiotics. We performed the disk diffusion assay using 0.1 mg / ml ampicillin as a positive control and distilled water and ethanol as a negative control and determined the following three results. First, the antibacterial activity of B material (DH) among the four commercial antibiotics against five strains was shown to be highest one. Second, the antibacterial activity of the ethanol extract of cinnamon was shown wide antibacterial activity against Gram negative and Gram positive bacteria, respectively. Specifically, the antibacterial activity of cinnamon-ethanol extract was about 75 ~ 153% as compared with that of the positive control under 100,000 ppm treatment conditions. Third, the antibacterial activities of commercial antibiotics and cinnamon extracts were shown slight differences to Gram negative bacteria and Gram positive bacteria. That is, the Gram(-) bacteria including E. coli showed a high antibacterial activity of 133 ~ 153% compared with the control group. Also, the range of antibacterial activity on Gram(+) bacteria was 75 ~ 122%. As a result, we suggest the potential of using cinnamon extract as a substitute for antibiotics through comparison of antibacterial activity with commercial antibiotics

Life cycle analysis on correlation relationship between GHG emission and cost of electricity generation system for energy resources

김희태(Kim, Heetae),안태규(Ahn, Tae Kyu) 한국신재생에너지학회 2011 한국신재생에너지학회 학술대회논문집 Vol.2011 No.05

In this work, we analyzed correlations between life-cycle greenhouse gas (GHG) emissions and life-cycle cost of energy resources. Energy resources studied in this paper include coal, natural gas, nuclear power, hydropower, geothermal energy, wind power, solar thermal energy, and solar photovoltaic energy, and all of them are used to generate electricity. We calculated the mean values, ranges of maximum minus minimum values, and ranges of 90% confidence interval of life-cycle GHG emissions and life-cycle cost of each energy resource. Based on the values, we plotted them in two dimensional graphs to analyze a relationship and characteristics between GHG emissions and cost. Besides, to analyze the technical maturity, the GHG emissions and the range of minimum and maximum values were compared to each other. For the electric generation, energy resources are largely inverse proportional to the GHG emission and the corresponding cost.

구강 미생물 7종에 대한 멘톨과 불소치약의 항균 활성 비교 연구

조세희(Cho, Sehee),안태규(Ahn, Tae-Kyu),윤찬건(Yun, Chan-Gun),김지우(Kim, Jiwoo),전예담(Jeon, Yedam),황진서(Hwang, Jinseo),김지인(Kim, Jee-In),이현경(Lee, Hyeongyeong),임재환(Lim, Jae-Hwan) 한국과학영재교육학회 2020 과학영재교육 Vol.12 No.3

구강에는 600종 이상의 미생물이 존재하는 것으로 보고되고 있다. 알코올과 항생제 제품을 대체하여 천연물을 사용한 구강 위생 제제의 개발연구가 보고되고 있으나, 특정 성분들의 균주별 항균 활성 등에 관한 연구는 부족하다. 본 연구에서는 멘톨, 녹차 추출물, 불소치약, 플루오린화나트륨을 사용하여 구강 미생물 7종 -S. mutans, E. coli, B. subtilis, S. aureus, B. cereus, P. aeruginosa, L. brevis- 에 대한 항균효능을 확인하고자 하였다. 디스크 확산법을 통해 멘톨(200 mg/mL)은 구강 미생물 7종, 치약의 경우는 2종의 균주 -S. mutans, B. cereus- 에서 10.4~ 21.9 mm의 clear zone이 형성되는 것을 확인하였다. 불소치약과 멘톨의 복합 처리 시에는 구강 미생물 4종에서 증가된 항균 활성을 확인하였다. 또한 MIC 분석을 통해, 멘톨은 200~500 ㎍/mL의 농도에서 모든 균주에 대한 최소억제농도를 확인할 수 있었고 플루오린화나트륨의 경우 E. coli에서 40 ㎍/mL의 억제농도를 확인할 수 있었다. 결과적으로, 구강청결제 및 구강질환 항생물질의 대체소재로서 멘톨의 활용 가능성을 제시하고자 한다. More than 600 kinds of microorganisms exist in the oral cavity. Studies on the development of oral hygiene preparations using natural products as a substitute for alcohol and antibiotic products have been reported, but studies on the antibacterial activity of specific ingredients by strain are insufficient. In this study, menthol, green tea extract, fluoride toothpaste, and sodium fluoride were used against 7 kinds of oral microorganisms, S. mutans, E. coli, B. subtilis, S. aureus, B. cereus, P. aeruginosa, L. brevis. Using the disk diffusion method, It was confirmed that clear zones of 10.4 to 21.9 mm are formed in 7 types of oral microorganisms when menthol (200 mg/mL) is treated and 2 strains, S. mutans and B. cereus, when treated with fluoride toothpaste. When combined treatment with fluoride toothpaste and menthol, increased antibacterial activity was observed in four oral microbes. Through the MIC analysis, the minimum inhibitory concentration of menthol for all strains was confirmed at a concentration of 200-500 ㎍/mL, and the inhibitory concentration of sodium fluoride was confirmed at 40 ㎍/mL in E. coli. As a result, we suggest that the possibility of using menthol as a substitute for oral cleansers and antibiotics for oral diseases.

의도적인 중독 환자들의 음주 여부에 따른 중독 중증도 지수의 비교

전민재 ( Min Jae Jun ),안태규 ( Tae Kyu Ahn ),강강수 ( Soo Kang ) 대한임상독성학회 2021 대한임상독성학회지 Vol.19 No.1

Purpose: Alcohol ingestion enhances impulsivity and aggression, and has been proven to have a close relationship with suicide. This study investigates whether alcohol co-ingestion affects the Poisoning Severity Score (PSS) grade in patients with intentional poisoning. Methods: We conducted a retrospective analysis of intentional poisoning patients who visited the emergency department (ED) from January 1 to December 31, 2020. Patients were divided into non-drunken and drunken groups. We collected the data based on the medical records of the patients and serum ethanol level results recorded during initial blood tests at the ED. To grade the PSS, the highest score was assessed through clinical signs and test results during the hospital stay. A comparative analysis was conducted between the two groups. Results: A total of 277 patients were included in the study. 163 (58.8%) were in the non-drunken group, and 114 (41.2%) were in the drunken group. The PSS grade showed a significant difference between the two groups (p=0.002). While grade 1 (mild) was observed more in the non-drunken group, grade 2 (moderate) and grade 3 (severe) were seen more in the drunken group. In an ordinal logistic regression analysis, alcohol co-ingestion (adjusted odds ratio [aOR] 2.557, 95% confidence interval [CI] 1.554-4.208, p<0.001) was considered to be a risk factor for a higher PSS grade. There was no significant correlation between the serum ethanol level and the PSS grade. (p=0.568) Conclusion: Intentional poisoning patients with alcohol co-ingestion had a higher PSS. Hence close observation and aggressive treatment in the ED is warranted in such cases.

산약에서 분리한 β-sitosterol과 daucosterol의 RAW 264.7 세포와 TK-1 세포에서의 면역 활성 조절 효능

박민경(Min-Kyung Park),조세희(Sehee Cho),안태규(Tae-Kyu Ahn),김도현(Do-Hyun Kim),김소연(So-Yeon Kim),이진욱(Jin-Wook Lee),김지인(Jee-In Kim),서을원(Eul-Won Seo),손건호(Kun-Ho Son),임재환(Jae-Hwan Lim) 한국생명과학회 2020 생명과학회지 Vol.30 No.4

많은 면역 소재 연구에서 RAW 264.7 세포를 이용한 결과는 많이 보고되어 있으나 T 세포 유래 TK-1 세포를 활용한 연구결과는 거의 보고되어 있지 않다. 또한 식물 유래 성분인 β-sitosterol의 효능 연구에 비하여 그 유사체인 daucosterol의 면역 활성 조절 기능에 관한 연구도 매우 부족하다. 본 연구에서는 산약, D. batatas, 에서 β-sitosterol과 daucosterol을 추출하여 분리하고 NMR 방법으로 동정하였다. 이후 분리된 phytosterols의 면역 증강 또는 억제 효능을 규명하기 위해 lipopolysaccharide로 염증반응이 유도된 RAW 264.7 세포와 TK-1 세포에 β-sitosterol과 daucosterol을 각각 농도별로 12시간 동안 처리한 후에 염증관련 유전자인 COX-2, TNF-α, IL-6, iNOS의 상대적인 발현양을 RT-PCR 방법으로 분석하였다. 대식세포주인 RAW 264.7에 β-sitosterol을 처리하였을 때, LPS만을 처리한 대조군의 발현 증가량과 비교하여 TNF-α와 iNOS의 상대적인 발현양이 3 fold 이상 증가하였다. TK-1 세포의 경우에서는 β-sitosterol의 처리 농도의존적으로 TNF-α의 발현양은 감소하고 iNOS의 발현양은 증가하였다. Daucosterol을 RAW 264.7 세포에 처리한 경우 COX-2, TNF-α, IL-6의 발현양이 0.7~1.2 fold 정도로 변화하였으며 iNOS의 경우는 특이하게 0.8~0.18 fold로 발현양이 농도의존적으로 감소하였다. TK-1 세포에 daucosterol을 처리한 경우에는, 놀랍게도 LPS만 처리된 경우와 비교하여 상대적으로 TNF-α, IL-6, iNOS의 발현량이 급격하게 감소하는 것을 확인하였다. 결과적으로, β-sitosterol의 처리는 RAW 264.7 세포에서 TNF-α, iNOS의 발현 증가를 유도하여 면역 상승 효과에 관여하였다. TK-1 세포에서는, iNOS 발현 수준이 증가하였고, 반면에 TNF-α 발현수준은 감소하여, β-sitosterol의 면역 억제 활성을 보여주었다. Daucosterol은 RAW 264.7 세포의 iNOS 발현양을 억제하고 TK-1 세포에서 TNF-α, IL-6, iNOS의 발현을 크게 억제함으로써, 대식세포와 T 세포주에서 모두 면역 억제 효능을 보이는 것으로 판단된다. Although many studies on immune modulatory materials have used RAW 264.7 cells, few have used T cell-derived TK-1 cell lines. Moreover, although some studies have investigated the efficacy of plantderived β-sitosterol, few have examined the immunomodulatory activity of its analogue, daucosterol. In this study, β-sitosterol and daucosterol were isolated from D. batatas and identified by nuclear magnetic resonance spectroscopy. To evaluate the immune-enhancing or inhibitory effects of the isolated phytosterols, the expression levels of the inflammatory response genes COX-2, TNF-α, IL-6, and iNOS were analyzed by RT-PCR. The relative expression levels of TNF-α and iNOS in RAW 264.7 cells were increased more than threefold with β-sitosterol treatment comparing to those of untreated control. In the case of TK-1 cells, the expression level of TNF-α was decreased and the expression level of iNOS was increased in a β-sitosterol concentration-dependent manner. The expression levels of COX-2, TNF-α, and IL-6 increased by approximately 0.7-1.2 times in RAW 264.7 cells treated with daucosterol compared to those of untreated control, but iNOS expression decreased by 0.8-0.18 times. In the case of daucosterol-treated TK-1 cells, the expression levels of TNF-α, IL-6, and iNOS were markedly reduced from those of TK-1 cells treated only with lipopolysaccaride. As a conclusion, β-sitosterol treatment increased TNF-α and iNOS expression levels in RAW 264.7 cells, thus exerting an immune- boosting effect. However, in TK-1 cells, iNOS expression increased while TNF-α expression decreased, indicating an immunosuppressive activity of β-sitosterol. Daucosterol appears to exert an immunosuppressive effect in both macrophages and T cell lines by inhibiting iNOS expression in RAW 264.7 cells and greatly inhibiting the expression of TNF-α, IL-6, and iNOS in TK-1 cells.

Correlation Analysis on CO₂ Emission and Cost of Energy Resources and Life Cycle Assessment

김희태(Kim, Heetae),김은철(Kim, Eun Chul),안태규(Ahn, Tae Kyu) 한국신재생에너지학회 2010 한국신재생에너지학회 학술대회논문집 Vol.2010 No.06

The world is moving towards a post-carbon society and needs clean and renewable energy for sustainable development. There are many methodological approaches which are helping this shift based on analyzed data about energy resources and which focus on limited types of energy including liquid fossil, solid fossil, gaseous fossil, and biomass (e.g. IPCC Guidelines, ISO 14064-1, WRI Protocol, etc.). We should also consider environmental impact (e.g. greenhouse gas emissions, water use, etc.) and the economic cost of the renewable energy to make a better decision. Recently, researchers have addressed the environmental impact of new technologies which include photovoltaics, wind turbines, hydroelectric power, and biofuel. In this work, we analyze the environmental impact with a carbon emission factor to present a correlation between CO₂ emission and the cost of energy resources standardized by the energy output. In addition, we reviewed Life Cycle Assessment (LCA) as another methodology. Researchers who are studying energy systems have ignored the impacts of entire energy systems, e.g. the extraction and processing of fossil fuels. In power sector, the assessment should include extraction, processing, and transportation of fuels, building of power plants, production of electricity, and waste disposal. Therefore LCA could be more suitable tool for energy cost and environmental impact estimation.

Driving factors in final energy use in Korean cities

Minjin Lee(이민진),Claudio Tenreiro,Tae Kyu Ahn(안태규) 한국신재생에너지학회 2012 한국신재생에너지학회 학술대회논문집 Vol.2012 No.06

제조에 對한 文獻的 考察

안태규,최병렬,송기철,이용연,유화승,서상훈,최우진,조정효,이연월,손창규,조종관 대전대학교 한방병원 2001 惠和醫學 Vol.10 No.1

In the literatual study on Holotrichia, the results were obtained as follows ; 1. Holotrichia is larva of Holotrichia diomphalia Bates etc. powder or liquor of Holotrichia is used medically. 2. Appearance of Holotrichia is shape of kidney, yellowish color. 3. The oriental characters of Holotrichia is warm, toxicant, salty. 4. The significant efficancy of Holotrichia is breaking the stagnant blood. 5. Holotrichia can be applied to the diseases related to thrombosis, and recover the demage of liver. 6. Holotrichia avails Liver diseases such as Hepatitis, Liver cirrhosis, Hepatosplenomegaly, Hepatoma etc.