Xylanase 첨가에 따른 수수의 제빵 적성 변화

안지은,고지연,고봉경,Ahn, Ji Eun,Go, Ji Yeon,Koh, Bong Kyung 한국식품조리과학회 2015 한국식품조리과학회지 Vol.31 No.1

This study investigated the baking properties of sorghum with the addition of xylanase or Pentopan, which is a baking additive containing xylanase. The control bread was made with a 30% substitution for wheat flour and the optimum level of enzyme addition was 0.75 mg/g flour for Pentopan and 5 mg/g flour for xylanase. The water binding capacity of wheat flour increased with the addition of sorghum, but decreased with the addition of either xylanase or Pentopan. The resistance of dough increased while extensibility decreased with the addition of sorghum; however, resistance decreased while extensibility increased with the addition of the enzyme. Specific volume of bread decreased significantly with the addition of sorghum. However, the specific volume was significantly recovered with the addition of enzyme. Crumb firmness was higher in the sorghum-added sample, but crumb firmness of the bread decreased with the addition of the enzyme. The crumb firmness of bread with added xylanase decreased significantly in 24 hours. These results demonstrated that adding sorghum with either xylanase or Pentopan that included xylanase increased specific volume and decreased crumb firmness whereas sorghum decreased the quality of fermented bread when added to wheat flour. The firmness rate of fermented bread particularly decreased with the addition of pure xylanase.

Miseq 시퀀서를 이용한 부후된 신갈나무 내 곰팡이 군집 분석 및 미생물 분리

안지은 ( Ji-eun An ),박미진 ( Mi-jin Park ),장석윤 ( Seokyoon Jang ),이수민 ( Soo Min Lee ) 한국목재공학회 2020 한국목재공학회 학술발표논문집 Vol.2020 No.1

본 연구는 변색 및 부후와 같이 목재 자원으로써 가치를 낮추는 요인에 대해 미생물학적 접근법을 이용한 분석을 통해 목재품질 최적화라는 목표에 기여를 하고자 수행하였다. 신갈나무(Quercus mongolica)를 공시재료로 사용하였으며 부후와 같이 결함이 생긴 피해목과 정상목을 각각 실험군과 대조군으로 사용하여 미생물 군집분석을 수행하였다. ‘문’ 수준의 곰팡이 군집분석에서는 9개의 분류군이 조사되었으며, 우점은 크게 Ascomycota (53.36 ~ 80.13%), Basidiomycota (2.84 ~ 32.77%), Fungi_p (1.7 ~ 26.84%) 3개로 조사되었다. 특히 정상목 대비오염목에서 Basidiomycota의 검출비율이 3.84 ~ 39.35% 증가됨을 확인하였는데, 이를 통해 Basidiomycota문에 속한 균류들이 목재의 부후에 영향을 주는 문임을 추정할 수있었다. ‘속’ 수준의 균류 군집분석 결과 369 분류군이 검출되었으며 Capnodiales_g (1.25 ~ 12.82%), Leptodontidium (0 ~ 11.85%), Mycena (0 ~ 22.21%), Phaeoacremonium (0.16 ~ 26.67%) 의 군집조성이 우점함을 확인하였다. 특히 Mycena 속의 경우, 정상목대비 일부 오염목에서 높은 비율로 검출된 결과를 통해 일부 오염목의 부후에 직접적으로 관련 있는 ‘속’임을 추정할 수 있었다. 부후된 신갈나무로부터 곰팡이의 직접 분리를 통해 자낭균류 26종, 털곰팡이문 5종, 담자균류 3종을 분리하였으며, 미생물 군집분석 결과에서 부후목재에 우점하였던 담자균문에 해당하는 곰팡이는 Clitopilussp., Sistotrema brinkmannii, Schizophyllum commune가 있음을 확인하였다.

g-C(3)N(4)기를 가진 카본나노튜브의 산소 환원 반응 특성

안지은 ( Ji Eun Ahn ),김현종 ( Hyun Jong Kim ),한명근 ( M. K. Han ) 한국공업화학회 2010 응용화학 Vol.14 No.2

g-C(3)N(4)/CNT as non-platinum catalysts was thermally synthesized for ORR catalyst. The key properties of CNT for electrochemical devices are good electrical conductivity and large surface area with high porosity. ORR onset potentials of g-C(3)N(4) and g-C(3)N(4)/CNT catalysts were approximately 0.64 V (NHE) and current density was 4.5 mA/cm(2). As increasing the thermal treatment up to 600℃ the catalytic activity was strikingly improved.

김치에서 박테리오신을 생산하는 Lactobacillus sakei B16의 분리 및 특성 분석

안지은(Ji Eun Ahn),김진경(Jin Kyoung Kim),이형로(Hyeong Rho Lee),엄현주(Hyun-Ju Eom),한남수(Nam Soo Han) 한국식품영양과학회 2012 한국식품영양과학회지 Vol.41 No.5

본 연구는 김치 내에서 균들의 생육을 조절하고 김치의 저장성과 안전성을 향상시키고자 김치로부터 항균활성이 우수한 박테리오신 생산 유산균을 동정 및 박테리오신의 특성을 확인하고자 하였다. 그 결과 항균활성이 뛰어난 Lb. sakei B16을 선발하였고 이 균주에서 생산되는 박테리오신은 pH와 열에 안정하고 일반적으로 박테리오신이 그람 양성균을 저해하는 것에 비해 본 연구에서 분리한 박테리오신은 그람 음성 유해균을 저해하여 넓은 저해 범위를 갖는 장점이 있다. 반면, trypsin, proteinase K, α-chymotrypsin을 처리 하였을 때 항균 활성이 저하되는 것을 확인하였다. 따라서 본 연구에서 분리한 박테리오신은 다양한 환경 변화에서 안정하고 체내에 섭취되었을 때 단백질 가수분해 효소에 의해 분해됨으로써 인체에 영향을 주지 않을 것으로 생각된다. 또한 sakacin P cluster 염기서열 정보를 이용하여 Lb. sakei B16에서 박테리오신 유전자의 존재를 확인하였다. 본 연구를 통해 유산균이 생산하는 박테리오신을 이용하여 김치 제조 산업에서 스타터로 개발하여 김치 내에서 미생물의 생육을 조절하고 김치의 저장성과 품질을 향상시킬 수 있을 것이라 기대된다. Lactic acid bacteria (LAB) are able to secrete antimicrobial peptides called bacteriocins, which inhibit other bacteria such as pathogenic microorganisms. Therefore, bacteriocin-producing starters can be used as natural biopreservatives for various foods. The objective of this study was to screen and characterize bacteriocin-producing LAB from Kimchi and to investigate their applicability as a starter in Kimchi fermentation. To screen bacteriocin-producing LAB, gram-positive and gram-negative bacteria were used as indicators. To measure the antimicrobial activities of isolates, agar well diffusion assay method was used. According to the results, bacteriocin produced by Lb. sakei B16 showed antimicrobial activity against Listeria monocytogenes ATCC 19115, Escherichia coli KCTC 1467, and Lactobacillus plantarum KTCT 3104. Furthermore, bacteriocin was very stable after treatment with high temperature and high and low pH, but its effects were inhibited by treatment with proteolytic enzymes such as trypsin, proteinase K, and α-chymotrypsin, revealing their bacteriocin-like protein-based structure. These results suggest that Lb. sakei B16 and its bacteriocin are good candidates as a functional probiotic and natural biopreservative, respectively, in fermented foods.

비대칭 하이브리드 커패시터 전극으로서의 전도성고분자-CNT 복합재료 합성 및 특성화

안지은 ( Ji Eun Ahn ),문지훈 ( Ji Hoon Moon ),김현종 ( Hyun Jong Kim ),한명근 ( M. K. Han ),설용건 ( Yong Gun Shul ),김성빈 ( Sung Bin Kim ) 한국공업화학회 2011 응용화학 Vol.15 No.1

Polypyrrole-CNT as supercapacitor was composed with MnO2-CNT for asymmetric hybrid capacitor. To improve the voltage characteristics were used of polypyrrole-CNT, compared to the conventional capacitor was ascertained that the high voltage range. Polypyrrole-CNT composite electrodes formed the peaks by the doping-dedoping for Li-ion.

신 수산통상규범 하 한-미 수산정책 비교 연구

안지은(Ji Eun An),박세현(Se Hyun Park),박명섭(Myong Sop Pak) 한국해양비즈니스학회 2021 해양비즈니스 Vol.- No.48

The U.S. is a major country that has introduced fisheries norms in major trade agreements such as CPTPP and USMCA, starting with the TPP discussion that began in 2010. In particular, with the inauguration of the Joe Biden administration in 2021, it is expected that US trade policies will shift toward supporting environmental issues, international cooperation, fairness and multilateral trade systems. This implies the possibility that the U.S. will push forward with the TPP negotiations again or expand and push forward the CPTPP, which has already officially entered into force in 2018. In this case, it is likely that the US-led CPTPP or the USMCA agreement, which adopted a higher level of discipline than the existing CPTPP in the fishery sector, will serve as a reference. In other words, as the US administration changes in the future, it is expected that the US s interest and influence in the formation of international fisheries norms will increase. Therefore, in preparation for the possibility that fishery regulations at the CPTPP and USMCA levels will expand to the world in the future, this study reviewed the fishery regulations newly emerged in the current trade agreement. In addition, the current state of fisheries policy in the United States, which is leading the discussion on international fisheries norms, and Korea, which are expected to have direct or indirect impacts amid such changes in international fisheries norms, was analyzed. Through this, this study attempted to derive complementary points and limitations of domestic fisheries policies to proactively respond to the impact of the expansion of fisheries provisions at a level similar to that of CPTPP and USMCA.

GDL 표면에 펄스 도금을 통한 PtCo 합금 전극의 제조 및 이를 이용한 연료전지 특성 평가

안지은 ( Ji Eun Ahn ),김현종 ( Hyun Jong Kim ),설용건 ( Yong Gun Shul ),이호년 ( Ho Nyun Lee ) 한국공업화학회 2011 응용화학 Vol.15 No.2

The pulse electro-deposition processes created the PtCo alloy catalysts directly on the surface of gas diffusion layer (GDL). The PtCo alloy catalysts was well dispersed in GDL. By increasing cobalt contents, the size of PtCo alloy catalysts was decreased, result in the enhancement of ESA. Enhanced performance and durability of platinum might be due to the both of structural change and decrease of particle size of platinum.

아세트산 처리가 발효균주 Saccharomyces cerevisiae와 Issatchenkia orientalis의 생장 및 발효효율에 미치는 영향

안지은(Ji-Eun An),이동민(Dongmin Lee),정한섭(Hanseob Jeong),이수민(Soo Min Lee) 한국산림바이오에너지학회 2019 산림바이오에너지 Vol.29 No.1

목질계 바이오매스의 초임계수 처리 후 얻어진 당화액 내에는 유기산 및 Furfural, 5-hydroxymethylfurfural과 같은 다양한 부산물들이 존재한다. 이러한 부산물은 효모의 성장 및 에탄올 발효를 저해시키므로 효율적인 에탄올 발효를 위해서는 당화액 내 저해물질을 제거하는 것이 중요하다. 당화액의 정제공정은 공정에서 생산단가 상승으로 이어지기 때문에 당화액 내 발효저해물질의 농도를 결정하는 것이 필요하다. 이전 연구에 의하면 목탄을 이용하여 저해물질을 약 95% 이상 제거한 바 있으나, 어렵게 얻어진 당의 손실과 여전히 당화액에는 부산물이 존재하여 미생물을 이용한 에탄올 발효 과정에서 발효균주의 생장과 발효효율 저해 요인으로 작용하고 있다. 따라서 본 연구에서는 당화액에 포함되어있는 저해물질 중 아세트산을 대상으로 당화액 내 농도변화에 따른 효모의 생장 및 발효 효율에 미치는 영향을 조사하였다. 구체적으로는 상업용 에탄올 생산 균주인 I. orientalis와 S. cerevisiae를 이용하여 아세트산이 0, 1, 2, 4, 8%가 포함된 조건에서 성장 및 발효능을 조시하였고, 그 결과 I. orientalis의 경우 4%, S. cerevisiae의 경우 2%가 균주 생장을 위한 최대 한계농도임을 확인하였으며, 발효효율에도 직접적인 영향을 미치는 것을 확인하였다. There are various byproducts in the hydrolysate, such as organic acids and furfural and 5-hydroxymethylfurfural, obtained through the supercritical water treatment of woody biomass. Since these byproducts are well known to inhibit yeast growth and ethanol fermentation, it is important to effectively remove these inhibitors from the hydrolysate solution. It s also necessary to determine the concentration of the fermentation-inhibiting substance in the glycated liquid because the purification process of the hydrolysates of woody biomass leads to the increase in the production cost of bioethanol and significant sugar loss in hydrlysates. Previous studies showed that about 95% of the inhibitors were removed using charcoal, but the loss of sugars also occurred. After charcoal treatment certain amount of byproducts still existed in the sacharification solution, which inhibit fermentation efficiency during the fermentation process using microorfanisms. In this study, the effects of acetic acid concentration on the growth and fermentation efficiency of yeast were investigated to understand tolerance of fermentation yeast strains. Specifically, growth and efficacy of two commercial ethanol productions strains, e.g. I. orientalis and S. cerevisiae were investigated under the conditions of 0, 1, 2, 4, and 8% acetic acid. As a result, I. orientalis 4% and S. cerevisiae 2% was the maximum limiting concentration for the growth of the strains.

연기자 오디션 프로그램 <기적의 오디션>의 비판적 고찰 - 배우의 정체성과 특수성의 몰이해를 중심으로

안지은 ( An Ji-eun ) 한양대학교 공연예술연구소 2017 공연예술연구 Vol.4 No.-

본 논문은 연기자 오디션 프로그램 <기적의 오디션>의 방송 구조를 살펴보고 프로그램에 대한 객관적인 평가를 바탕으로 실패원인을 분석하는 데 목적을 둔다. 프로그램에 대한 평가와 반응을 살펴 본 결과 전문가와 비전문가의 동일한 평가로, 시청률의 저하, 프로그램 구성에 대한 비평, 변화하는 심사기준에 대한 지적 뿐 아니라 프로그램 종료 후 최종 우승자에게 약속된 주연급 캐스팅 불발 및 우승자의 활동 부재는 결과적으로 프로그램의 실패를 증명해 준다. 실패원인을 분석해 본 결과, `배우의 정체성의 몰이해`, `연기의 특수성을 배제한 리얼리즘 프로그램에서의 극 재현`, `시청률을 의식한 제작진의 의도된 연출의 오류`로 드러났다. 또한 배우를 선발하는 연기자 오디션 프로그램은 기존 가수 오디션 프로그램의 정형적인 포맷을 차별성 없이 답습한 것이 가장 큰 실패요인으로 분석 된다. 결국 `연기`라는 전문적인 예술분야에 대한 개념 이해와 특수성을 신중히 고찰하지 않은 것은 프로그램의 실패라는 결과를 초래 했다. 본 연구자는 리얼리즘 프로그램에서 비춰지는 배우 즉, `배우의 정체성`과 `연기의 특수성`에 대한 깊은 탐구와 해석의 필요성을 제안하며 예술인을 양성하는 오디션 프로그램의 질적 향상을 기대해 본다. This thesis examines the broadcasting structure of the acting audition TV program Miracle Audition and aims to analyse the cause of its failure based on an objective evaluation of the program. Evaluations of and reactions to the program were gathered from experts and non-experts alike, proving that the program`s failure was due to a deteriorating rating, criticism of the program structure, changing assessment criteria, and a failure to deliver the major roles promised to the winner after the end of the program, leading to an absence of activities by the winner. These causes of failure were analysed with reference to the following categories: `actors` lack of understanding of identity`, `actual replay in a reality program that excludes acting specialization`, and `internal errors within the production process by the production crew who were conscious of audience ratings`. Analysis showed that the biggest factor behind the show`s failure was that it followed the same regular format of existing singing audition programs without differentiation, even though this acting audition program was intended to select actors, a clearly different profession. In the end, neglecting to carefully develop a conceptual, specialized understanding of the professional, artistic field of acting resulted in the failure of the program. This research suggests the need for deeper exploration and interpretation of the role of actors in reality programs, namely, `the identity of the actor` and `the unique aspects of acting`. It can be hoped that pursuing these topics would allow for improvement in the quality of audition programs to cultivate artists.

미국-멕시코-캐나다 협정(USMCA) 수산조항에 관한 연구

안지은(Ji Eun An),정명화(Myoung-Hwa Jung) 한국해양비즈니스학회 2020 해양비즈니스 Vol.- No.45

On March 13, 2020, the domestic approval process for Canada, the last ratified member of the United States-Mexico-Canada Agreement (USMCA), was completed. Accordingly, the USMCA, signed on October 1, 2018, will be officially into force within the next three months. This means that the North American Free Trade Agreement (NAFTA) system, which has been in charge of trade policy between the three notrh american countries in the past 25 years, has ended, and a new trade environment called the USMCA has been created. The USMCA is the result of the renegotiation of the NAFTA, a key commitment highlighted by the US Trump government, and is a representative product of the US government s policy to strengthen protectionism. Due to the strong commitment of the US to renegotiate NAFTA, the the United States have took the lead of the USMCA in the form of bilateral agreements with Mexico and Canada respectively. In addition, the structure and contents of the agreement have similar characteristics to the TPP, which the US led in the past. The USMCA Fisheries Provisions are contained in Chapter 24(Environment) of the Agreement and consist of a total of five provisions: the importance of sea catching fisheries, fisheries management, marine life conservation, fisheries subsidies regulation and notification, and IUU fishing regulations. The USMCA Fisheries Provisions have the following important meanings in the formation of international fisheries norms. First, it is the only multilateral trade agreement signed by the United States, including specific and comprehensive fisheries regulations. Second, it is likely to be the basis for establishing fisheries regulations under bilateral and multilateral agreements, such as the future FTA and WTO fishery subsidies negotiation.