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보조 형광증가제를 이용한 L-Dopa의 형광분광법적 정량
이상학,안정미,Lee, Sang Hak,Ahn, Jung Mi 대한화학회 2000 대한화학회지 Vol.44 No.6
리간드 증감 유발 형광법을 이용하여 Tb(III)-L-dopa (L-3,4-dihydroxyphenyl alanine) 착이온의 방출세기를 측정함으로써 수용액 중의 L-dopa를 정량하는 방법에 대하여 연구하였다. 들뜸파장, pH, 보조 형광증가제의 선택, Tb(III) 이온의 농도, 보조 형광증가제로 사용된 Lu(III) 이온의 농도 및 방출파장의 방출세기에 대한 영향을 조사하였다. 보조 형광증가제로서 Lu(III) 이온을 첨가하였을때 Tb(III) 이온의 방출세기가 현저히 증가함을 관찰하였고, L-dopa의 검출한계를 낮출 수 있었다. 보조 형광증가제를 첨가하지 않았을 경우에 L-dopa 검정곡선의 직선감응범위는 들뜸파장, pH 및 Tb(Ⅲ) 이온의 농도가 각각 300 nm, 8.0 및 $1.0{\times}10^{-4}$ M였을때, $5.0{\times}10^{-7}$ M~$1.0{\times}10^{-4}$ M였다. 이 조건에서의 검출한계는 $4.0{\times}10^{-8}$ M였다. 보조 형광증가제를 첨가하였을 경우에는 들뜸파장, pH, Tb(III) 이온의 농도, 보조 형광증가제로 사용된 Lu(III) 이온의 농도 및 방출파장이 각각 300 nm, 8.5, $1.0{\times}10^{-5}$ M, $1.0{\times}10^{-5}$ M 및 545 nm였을 때, 직선감응범위가 1.0×$10^{-8}$ M~2.0{\times}10^{-4}$ M였고, 이 때의 검출한계는 $1.0{\times}10^{-9}$ M였다. Methods to determine L-dopa(L-3,4-dihydroxyphenylalanine) in aqueous solution by spectrofluorimetry based upon the ligand sensitized luminescence of Tb(III) ion L-dopa complex have been studied. Tb(III) ion and Lu(III) ion were used as ligand sensitized fluorescencer and co-fluorescence enhancer, respectively. The effects of excitation wavelength, pH, concentration of Tb(III) ion, concentration of Lu(III) ion and emission wavelength on the fluorescence intensity were investigated. The fluorescence intensity of the Tb(III) ion L-dopa complex was further increased with addition of Lu(III) ion. The calibration curve for L-dopa was linear over the range from 5.0 ${\times}$ $10^{-7}$ M to 1.0${\times}$ $10^{-4}$ M and the detection limit was 4.0 ${\times}$ $10^{-8}$ M under the optimal experimental conditions of 300 nm, 8.0, 1.0 ${\times}$ $10^{-4}$ M and 545 nm for excitation wavelength, pH, concentration of Tb(III) ion and emission wavelength, respectively. When Lu(III) ion was added to Tb(Ⅲ) ion L-dopa complex, the concentration range of linear response and detection limit were 1.0${\times}$$10^{-8}$ M to 2.0 ${\times}$ $10^{-4}$ M and 1.0 ${\times}$ $10^{-9}$ M, respectively under the optimal experimental conditions of 300 nm, 8.5, 1.0 ${\times}$ $10^{-5}$ M, 1.0 ${\times}$ $10^{-5}$ M, 545 nm for excitation wavelength, pH, concentration of Tb(III) ion, concentration of Lu(III) ion and emission wavelength, respectively.
해양 물리적 영향에 따른 강원도 해역 인공어초의 보존상태 조사
이지현(Ji-Hyun Lee),김완기(Wan-Ki Kim),안정미(Jung-Mi Ahn),이채성(Chae-Sung Lee) 한국마린엔지니어링학회 2009 한국마린엔지니어링학회 학술대회 논문집 Vol.2009 No.-
Stability and durability of the artificial reefs(ARs) for wildstock enhancement have been a key issue. We surveyed the stability of 8,740 artificial reefs (80 group AR's) installed at the Gangwon coast of the East Sea (Sea of Japan) through which 1 to 3 typhoons, wave heigh are passing annually. The ARs surveyed were 2 types aging 1996 to 2005 years. The side scan sonar(SSS) and scuba diving surveys confirmed that the ARs stability was age and type-dependent. A case of a confirmation amount to 84.8% ARs remained safe, the rest 15.2% being influenced by a physical parameter of the waters. The representative influences by their circumstances were of capsizing (8.1%), breakage (7.1%). A group of a AR's to 66.3% ARs remained safe, the rest 33.7% being influenced by a physical parameter of the waters. The representative influences by their circumstances were of sink (8.8%), burial (15.0%) and both of two Phenomenon(sink, burial) (10.0%).
김완기(Wan-Ki Kim),이지현(Ji-Hyun Lee),김대권(Dai-Kweon Kim),조규태(Qtae Jo),안정미(Jung-Mi Ahn),공용근(Yong-Geun Gong) 한국마린엔지니어링학회 2007 한국마린엔지니어링학회 학술대회 논문집 Vol.2007 No.-
Stability and durability of the artificial reefs (ARs) installed for wildstock enhancement .have been a key issue. We surveyed the stability of 4,044 artificial reefs installed at 5 sites (Gangeung, Donghae, Samcheok, Goseong, and Yangyang) along the Gangwon coast of the East Sea (Sea of Japan) through which 1 to 5 typhoons pass annually. The ARs surveyed were 7 types aging 1 to 4 years. The side scan sonar (55S) and scuba diving surveys confirmed that the ARs stability was age and type-dependent. A total of 85.56% ARs remained safe, the rest 14.44% being influenced by physical parameters of the waters such surf. An interesting observation was that the AR stability was apparently influenced by the next year's wave after installation rather than by age. The representative influences by their circumstances were capsized (0.20%), broken (5.79%), sunk (0.27%), and buried (8.18%). By type, dice reef was most stable, while semicircle branch reef was most vulnerable.
Bacillus subtilis 저온충격 단백질 Csp 유전자 결손이 저온 환경에서의 생장에 미치는 영향
안정미 ․ 이상수 배재대학교 자연과학연구소 2011 自然科學論文集 Vol.22 No.1
Cold shock proteins (CSPs) are expressed in cold conditions in order to adapt to that cold conditions. Three cold shock proteins CspB, CspC and CspD exist in Bacillus subtilis. To know the effect of cold environment on the cell growth each three cold shock gene was deleted. Growth curves in the conditions of 37℃ and 20℃ were compared to understand cold acclimation of these deleted mutant cells. Also, growing cells in the conditions of 37℃ to the log phase and then cold shock treatment which growing temperature was changed from 37℃ to 15℃ was carried out. The results of cold acclimation showed that both of the growth rate of wild type and Csp deleted mutants at 37℃ is nearly same but the growth rate of mutant cells was reduced compared to the wild type cell. On the other hands, both of wild type and mutant cells show nearly same growth rate on cold shock. These results demonstrate that Csp deleted mutants are not cold sensitive.
Bacillus subtilis 저온충격 단백질과 DEAD-Box RNA Helicase 유전자의 이중 결손 균주들의 저온 민감성 생장
안정미·이상수 배재대학교 자연과학연구소 2012 自然科學論文集 Vol.23 No.1
In order to understand the effect of interactions between cold shock proteins (CSPs: CspB, CspC, CspD) and cold inducing DEAD-box RNA helicases (YdbR, YqfR) on the cell growth at low temperature, we constructed double deletion mutants of these genes. The growth at 37℃ and 20℃ were compared to illuminate the cold sensitivity of these deletion mutants. Previously no cold sensitivity of csp single gene deletion mutants were shown but the growth rates of single deletion mutants of ydbR, yqfR DEAD-box RNA helicase genes were lower compared to that of the wild type strain at 20℃. Therefore additional deletion of csp genes on ydbR, yqfR deletion mutants were constructed and checked the growth rate at lower temperature. The results the growth rates of double deletion mutants of ydbR and csp genes reduced at lower temperature. It seems that the interactions between ydbR and csp genes effect on the adaptation at lower temperature.
김백남,나현희,안정미,안은영 대한감염학회 2005 감염과 화학요법 Vol.37 No.1
An outbreak of acute febrile reaction which developed after the administration of teicoplanin was identified in seven patients. Fever, with hypotension in some, developed during or soon after infusion and persisted for several hours. Laboratory abnormalities including leukocytosis and elevation of liver enzymes and creatine phosphokinase in serum were observed for two or three days. Contamination of the drug with endotoxins was identified.