Lipopolysaccharide 항원에 노출된 발생초기의 림프절내 B 및 T 림프구의 면역학적 변화

안금선(Ahn, Keum-Sun) 한국산학기술학회 2011 한국산학기술학회논문지 Vol.12 No.2

가슴샘 비의존성 항원이며 B 림프구의 분열원로 알려져 있는 lipopolysaccharide(LPS)를 발육초기의 C57BL/6 계 생쥐에 투여한 후 림프조직의 성숙과정에 따른 면역반응의 양상을 발생학적인 측면에서 형태학적으로 규명하고자 본 실험을 시행하였다. 1. 생후 0일과 3일에 LPS를 주사한 군에서는 주사 후 시간경과에 따라서 림프소절의 총수가 대조군에 비하여 증가되 지 않았다. 2. 생후 5일과 7일에 LPS를 주사한 후 2-4주가 경과되었을 때 림프소절의 총수는 대조군에 비하여 유의성있게 증가 하였다. 3. 생후 0일에서부터 1주 사이에 LPS를 주사한 후 1-4주가 경과된 실험군에서는 모든 림프절과 심층피질의 면적이 대조군에 비하여 약 1.5-3배정도 커졌다. 4. 생후 3일, 5일 및 1주에 LPS를 주사한 후 2-4주가 경과된 실험군에서는 면적이 0.1 mm2 이상인 림프소절들이 대 조군에 비하여 증가하였다. 5. 생후 5일과 1주에 LPS를 주사한 후 2-4주가 경과된 실험군에서는 면적이 0.01 mm2 미만인 림프소절들이 대조군 에 비하여 증가하였다. 이상의 실험결과로 미루어 볼 때 LPS의 자극에 의한 새로운 림프소절의 형성능력은 생후 5일-1주에 이루어지며, 또 한 새로 생성되는 림프소절들은 면적이 0.01 mm2 미만인 작은 림프소절들일 것으로 예측된다. This study was designed to clarify the morphometrical change of lymph node, deep cortex and lymph follicles in draining lymph nodes of young mice in response to local injection of lipopolysaccharide(LPS). 1. In the group stimulated with LPS, aged 0 day and 3 days, the number of lymph follicles were not significantly different from those of control group. 2. In the group two to four weeks after injection with LPS, aged five days and one week, the number of lymph follicles were significantly increased from those of control group. 3. In the group one to four weeks after injection with LPS, aged 0 day, three days, five days and one week, the area of lymph node and deep cortex increased about 1.5-3 times more than that of the control group. 4. In the group two to four weeks after injection with LPS, aged three days, five days and one week, the lymph follicles(the area: larger than 0.1 mm2) were increased from those of control group. 5. In the group two to four weeks after injection with LPS, aged five days and one week, the lymph follicles(the area: smaller than 0.01 mm2) were increased from those of control group. In view of these experimental findings, the formation of lymph follicles were induced by LPS stimulation from 5 days to one week after birth. The newley formed lymph follicles area in response to LPS may be less than 0.01㎟.

임상전단계실습 교과목의 개설시기 및 치과경험에 따른 참여태도, 스트레스, 만족도 분석

장미화 ( Mi Hwa Jang ),김주후 ( Ju Hu Kim ),안금선 ( Keum Sun Ahn ) 한국치위생학회(구 한국치위생교육학회) 2013 한국치위생학회지 Vol.13 No.1

Objectives: The purpose of this study was to examine the participation attitude of students toward pre-clinical practicums, their satisfaction with the practicums and related stress, and to investigate the influential factors for their participation attitude to practicums. Methods: The subjects in this study were three different groups of students who took pre-clinical practicums at different times. One group of students who entered college in 2008 took a practicum in December, 2009, and another group of students who entered college in 2009 took a practicum in June, 2010. The third group of students who entered college in 2010 took a practicum in June, 2011. Results: 1. As for participation attitude, the students who completed a practicum before they underwent field training in the second semester of their sophomore year got a higher mark of 3.44(p<0.05). 2 Concerning the operation of practicum, they felt a lot of stress when they took a practicum before they underwent field training in the first semester of their sophomore year and when they had no dental experience(p<0.001). 3. Regarding satisfaction with practicum, they scored higher when they took a practicum before they underwent field training in the second semester of their sophomore year(p<0.05). And the students who were more gratified with dental experience expressed better satisfaction(p<0.001). 4. As to the influential factors for the participation attitude of the students, those who were more satisfied with the content of practicum, the operation of practicum and evaluation took a more positive participation attitude(p<0.001). More stress about the operation of practicum and more stress about evaluation exerted a greater negative influence on participation attitude(p<0.05). Conclusions: Given the findings of the study, a pre-clinical practicum should be offered before field training is provided in the second semester of the sophomore year in order to ensure the efficiency of education, and a wide variety of information should be offered so that students could gain dental experience before they take a practicum.

구강보건인식도 및 관리실태에 관한 비교조사연구

안금선 경복대학 1998 京福論叢 Vol.2 No.-

본 연구는 우리나라 학교구강보건 활동을 재검토, 분석하여 새로운 학교구강보건의 방향을 설정하므로써 학생들의 구강건강을 유지, 증진시키고자 경기도 포천군 관내의 초등학교중 학교구강보건교육을 1회 실시한 후 6개월이 되는 136명을 실험군으로, 학교구강보건교육을 전혀 실시하지 않은 139명을 비교군으로 구강보건지식 수준 및 학교구강보건관리실태를 비교조사한 바 다음과 같은 결론을 얻었다. 1. 실험군과 비교군 학생들의 구강보건지식 수준은 낮았으며 실험군에서는 비교군보다 조금 높게 조사되었다. 2. 불소에 관한 인식에서는 실험군에서 38.2%가 불소를 알고 있었으며 비교군에서는 28.1%가 알고 있는 것으로 조사되었다. 3. 충치를 예방할 수 있는지에 관하여는 실험군에서 41.2%가 예방할 수 있다 답하였고 비교군에서는 28.1%만이 예방할 수 있다고 답하였으며, 실험군에서 47.8% 비교군에서 65.3%는 잘 모르겠다로 조사되었다. 4. 실험군의 60.6%, 비교군의 70.5%가 하루 2회 잇솔질하는 것으로 조사되었고 3회 잇솔질은 실험군에서 24.8%,비교군에서 15.5% 로 조사되었다. 5. 잇솔질시기에 관하여는 실험군에서 71.5%, 비교군에서 57.6% 가 식사후로 조사되었다. 6.구강건강 유지 방법에 관하여는 올바른 잇솔질이 실험군 64.2%, 비교군에서 60.4%로 가장 높게 조사되었다. 7. 학교구강보건교육을 위해서는 우선 일선교사와 양호교사에 대해 구강보건 교육이 강화될 필요가 있으며 장기적으로 치과위생사의 학교구강보건실 배치가 필요하다. This study reexamines and analyzes the activity of oral health on school in our country. And as it se s up the direction of new oral health on school, it is to keep and promote oral health of students. There are two groups in this study. One is case group with 136 students coming six months after enforeing the oral health education on school time among primary schools within the jurisdiction of Pochun-gun, Kyonggi-do. And the other is control group with 139 students who have oot enforced the oral health education on school at all. With these, this study was prepared for the comparative research on the degree of knowledge of oral health and the actual condition of management of oral health on school. And the results were as follows. 1. The degree of knowledge of oral health for students in case group and control group was low and case group was a little higher than control group. 2. In the recognition of fluorine 38.2% of case group, 28.1% of control group were aware of fluorine. 3. According to the fact, whether or not they can prevent teeth from decayingm 41.2% of case group and only 28.1% of control group answered that they could. And 47.8% of case group, 65.3% of control group answered that they couldn't. 4. 60.6% of case group, 70.5% of control group brushed their teeth two times a day. And 24.8% of case group, 15.5% of control group brushed their teeth three times a day. 5. According to the time for tooth-brushing 71.7% of case group, 57.6% of control group brushed their teeth after meal. 6. Accoding to the method of the oral health maintenance, the proper tooth-brushing was extremely high for 64.2% of case group and 60.4% of control group. 7. For the oral health education on school, first of all, it is necessary to strengthen the oral health education on teachers in the first line and nurse-teachers. And for the long run, it is necessary to arrange a dental hygienist at the oral health center on school.

K대학 치위생과 재학생의 학과만족도 조사 연구

송윤신,권순복,안금선,김영남,김수화,차민주 경복대학 2003 京福論叢 Vol.7 No.-

경기 K대학 치위생과 재학생들의 대학 진학시 학과선택 요인을 알아보고, 학과 만족도를 분석하여 치위생과 학과 경쟁력을 높이고 학생지도의 기초자료를 제공하고자 한다. 치위생과 입학 결정시 영향을 준 요인으로는 전반적으로 본인이 가장 높게 나타났으나 학년별로는 2학년이 77.5%로 가장 높았으며, 치위생과 선택이유로는 취업보장, 주위권유, 적성·흥미 순으로 조사되었다. 치위생과 학과만족도에서 강의실환경에 대해서는 전체 조사대상자 중 9.4%가 만족하다고 응답했고, 실험·실습기자재는 36.0%가 만족한다고 응답하였으며, 학우관계에서는 74.0%, 학과지도방침 38.9%, 치과위생사 직업만족도는 79.4%가 만족하고 있었다. 특히 조사대상자 중 다른 학년보다 2학년 학생들에게서 직업만족도를 제외한 다른 항목의 만족도가 가장 높게 나타나고 있다. 직업만족도는 1학년이 85.4%로 가장 높게 조사되었다. 휴학·자퇴를 고려한 경험유무에 대한 질문에서는 있다고 응답한 학생이 47.8%이었으며, 그 이유로 1학년은 경제적이유를, 2, 3학년은 적성이라고 응답하였다. 입학결정요인과 만족도와의 관계에서는 본인이 입학을 결정했을 때 재학만족도, 직업만족도, 학과지도방침, 전임교수에 만족한다고 응답한 경우가 가장 많았으며 진로계획에서는 취업이 가장 높았다. 학우관계와 만족도와의 관계에서는 학우관계에 만족한다고 응답한 경우 재학만족도, 직업만족도, 학과지도방침에 대한 만족도가 높은 것으로 분석되었다. 구강보건교육이 치위생과 선택에 미친 영향력에 대한 조사에서는 학년별 유의성은 보이지 않았으나, 전체조사대상 중 89.5%가 영향력이 보통이상이라고 응답해 높은 비율을 차지하는 것으로 나타났다. The purpose of this study is to identify the factors affecting selection of a major and the department satisfaction for improving the competitiveness of the dental hygiene department and providing the data for students guidance. The most influential person and factor in selecting a major is the person oneself(77.5% of the second-year students responded to the question) and the percentage of employment. The reaction of satisfaction with the classroom and practice facility indicates 9.4% and 36.0% in favor. 74.0% of surveyees is satisfied with classmates. In satisfaction with a course of department and profession, 38.9% and 79.4% of respondents answer in the affirmative. The second-year students of the respondents shows higher rate than other grades in .satisfaction with items above, except the satisfaction with profession. It appears that the freshman answered the most in that question. From the survey related to the experience of thinking over temporary absence from school or voluntary resignation, 47.8% of surveyees responded they had experienced thinking about that. The freshman answered the reason is financial difficulty and the second- and third-year students selected an aptitude. In the correlation coefficient of determinants for selection of college with the department satisfaction, when the determinant is oneself, the satisfaction with being at college, profession, a course of department, and teaching staff is high. In the correlation coefficient of classmates with department satisfaction, the students responding in the affirmative on classmate satisfaction had a high degree of satisfaction with being at college, profession, and a course of department. The experience of oral health education is not statically significant by grades to select the major, but 89.5% of the students majoring dental hygiene at K-college responded over average.