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시공간 클러스터링 분석을 이용한 2010~2011 국내 발생 구제역 전파양상
배선학,신연경,김병한,박선일,Bae, Sun-Hak,Shin, Yeun-Kyung,Kim, Byunghan,Pak, Son-Il 대한수의학회 2013 大韓獸醫學會誌 Vol.53 No.1
To investigate the transmission pattern of geographical area and temporal trends of the 2010~2011 foot-and-mouth disease (FMD) outbreaks in Korea, and to explore temporal intervals at which spatial clustering of FMD cases space-time analysis based on georeferenced database of 3,575 burial sites, from 30 November 2010 to 23 February 2011, was performed. The cases represent approximately 98.1% of all infected farms (n = 3,644) during the same period. Descriptive maps of spatial patterns of the outbreaks were generated by ArcGIS. Spatial Scan Statistics, using SaTScan software, was applied to investigate geographical clusters of FMD cases across the country. Overall, spatial heterogeneity was identified, and the transmission pattern was different by province. Cattle have more clusters in number but smaller in size, as compared to the swine population. In addition, spatiotemporal analysis and the comparison of clustering patterns between the first 7 days and days 8 to 14 of the outbreak revealed that the strongest spatial clustering was identified at the 7-day interval, although clustering over longer intervals (8~14 days) was also observed. We further discussed the importance of time period elapsed between FMD-suspected notice and the date of confirmation, and emphasized the necessity of region-specific and species-specific control measures.
윤순식,변재원,양동군,신연경,위성환,김병한,Yoon, Soon-Seek,Byun, Jae-Won,Yang, Dong-Kun,Shin, Yeun-Kyung,Wee, Sung-Hwan,Kim, Byounghan 대한수의학회 2010 大韓獸醫學會誌 Vol.50 No.3
Recently, the number of stray dogs is proportionate to the increase of the number of the companion dogs. Sarcoptic scabiei var canis, causing scabies, is one of the most important canine zoonotic arthropods in Korea and around the world. Thus, we have tried to know the prevalence of canine scabies in the stray dogs in Korea. A total of 565 stray dogs were collected from the rescue centers all over the country from Jan. 2006 to Dec. 2006. They were grouped with euthanasia or natural death and examined for the gender and age estimated by dental formula. To identify the lesions, the whole body was grossly examined and tested pathologically. Thirty two (5.66%) of 565 dogs were diagnosed as canine scabies. Dogs from urban areas had fewer scabies (0.62%) than those of rural areas (12.5%). Prevalence of scabies in male and female dogs was no difference as 5.96% and 5.25%. Euthanasia group showed higher prevalence (6.48%) than natural death group (2.44%) in scabies. Old dogs over five years showed lower infestration (1.82%) in scabies. In histopathological findings, there were mites in the burrows formed in the subcorneal space. Acanthosis, hyperkeratosis with crust, and vascular dilatation were main findings. One hundred thirteen (20%) of 565 stray dogs were diagnosed to have skin disease. Among them, canine scabies is the most prominent ectoparasite as 5.66 %. With previous reports on human infection in Korea, canine scabies must be regarded as the important zoonotic canine skin disease. Accordingly, for the human and canine hygiene it is imperative that stray dogs with skin problems are segregated and tested for the parasites to treat properly as soon as arriving at rescue shelter.
DNA 교차 오염 방지 기능을 가진 돼지 인플루엔자바이러스 감별진단용 one-step multiplex RT-PCR 진단법
김희정 ( Hee Jung Kim ),김은미 ( Eun Mi Kim ),신연경 ( Yeun Kyung Shin ),송재영 ( Jae Young Song ),김성희 ( Seong Hee Kim ),이경기 ( Kyoung Ki Lee ),이명헌 ( Myoung Heon Lee ),김영화 ( Young Hwa Kim ),박준철 ( Jun Cheol Park ) 한국동물위생학회 2014 韓國家畜衛生學會誌 Vol.37 No.4
In this study, we developed a cost and time saving one-step multiplex RT-PCR for the simultaneous detection and differentiation of swine influenza viruses (SIV) and 2009 pandemic influenza H1N1 virus (pH1N1). The one-step multiplex RT-PCR using four sets of primer was confirmed to be capable of detection of all SIV subtypes and differential diagnosis of major SIV subtype H1, H3 and pH1N1 on individual or mixed viral culture samples. The sensitivity of the multiplex RT-PCR was determined to be at least 2-6 HA/25 μL of the presented SIVs, providing sufficient efficacy for a routine SIV monitoring in diagnostic laboratories. In addition, compared with the conventional RT-PCR methods that cannot avoid the carryover DNA contamination, the developed RT-PCR applied with the uracil DNA glycosylase (UNG) system was proven to prevent a false positive reaction by carryover contamination of the pre-amplified DNA. In conclusion, the one-step RT-PCR with UNG system could be applicable to detect and differentiate of SIV from the viral cultures without worry of carryover DNA contamination in clinical laboratories.
등온증폭법을 이용한 돼지인플루엔자바이러스 신속 진단법 개발
김은미 ( Eun Mi Kim ),전효성 ( Hyo Sung Jeon ),김지정 ( Ji Jung Kim ),김희정 ( Hee Jung Kim ),신연경 ( Yeun Kyung Shin ),송재영 ( Jae Young Song ),여상건 ( Sang Geon Yeo ),박최규 ( Choi Kyu Park ) 한국동물위생학회 2015 韓國家畜衛生學會誌 Vol.38 No.2
In this study, we developed a rapid, sensitive and specific reverse-transcriptase loop-mediated isothermal amplification (RT-LAMP) assay for detection of swine influenza viruse (SIV) including major subtypes of swine influenza viruses H1N1, H1N2 and H3N2, and a novel subtype of influenza A virus that accidentally infected in pig population. The RT-LAMP was completed in 40 min at 58oC and the sensitivity of the RT-LAMP (1 copy/ L) was 10-fold higher than conventional reverse transcription-polymerase chain reaction (RT-PCR) (10 copy/ L) and the same to real time RT-PCR (1 copy/ L). Also, the result of the RT-LAMP can be confirmed without any detection system. Therefore, the RT-LAMP could be a alternative diagnostic method for SIV detection in national SIV monitoring system and clinical diagnostic laboratory in the future.