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SIS/PLGA 필름에서 SIS 함량이 신경계 세포의 부착과 증식에 미치는 영향
김초민 ( Cho Min Kim ),김순희 ( Soon Hee Kim ),송이슬 ( Yi Seul Song ),정수현 ( Su Hyun Jung ),김혜린 ( Hye Lin Kim ),오재민 ( Jae Min Oh ),박종학 ( Jong Hak Park ),이동원 ( Dong Won Lee ),이종문 ( John M Rhee ),강길선 ( Gil Son K 한국조직공학·재생의학회 2009 조직공학과 재생의학 Vol.6 No.4
Porcine small intestinal submucosa(SIS) derived from the submucosal layer of porcine intestine, both generate a minimal inflammatory response following implantation and have been approved by the FDA for many types of biomedical applications. Poly(lactic-co-glycolic acid)(PLGA) is a biodegradable synthetic polymer with acceptable mechanical strength and well-controlled degradation rate. Also, it can be easily fabricated into many shapes. In this study, we fabricated natural/synthetic biomaterial hybrid films using 0, 10, 20, 40 and 80 wt% of SIS. Schwann cells(SCs) and olfactory ensheathing cells(OECs) were seeded on SIS/PLGA film and confirmed the effects of adhesion and proliferation on SCs and OECs according to content of SIS. We confirmed that specific 10~20 wt% SIS in SIS/PLGA films was superior to adhesion and proliferation of SCs and OECs and content of SIS such as 40 wt% and 80 wt% could not help cell growth and proliferation.
슈반세포와 골수간엽줄기세포의 공배양이 골수간엽줄기세포의 분화와 증식에 미치는 영향(2)
김초민 ( Cho Min Kim ),김순희 ( Soon Hee Kim ),송이슬 ( Yi Seul Song ),정수현 ( Su Hyun Jung ),박종학 ( Jong Hak Park ),오재민 ( Jae Min Oh ),이동원 ( Dong Won Lee ),유일수 ( Il Sou Yoo ),이종문 ( John M Rhee ),강길선 ( Gil Son Kh 한국조직공학·재생의학회 2009 조직공학과 재생의학 Vol.6 No.4
Schwann cell(SC) play an important role as they are the source of different trophic substances and present a great neurotrophic activity. Bone marrow stromal cells(BMSCs) contain mesenchymal stem cell and progenitor cells. BMSCs can differentiate into various cell types, including muscle and brain cell. The aim of this study is to investigate the differentiation of BMSCs through co-culture system with SCs. Pure SCs and BMSCs were cultured as control group. Expression of GFAP, P75 and NF were determined by immunocytochemical staining and reverse transcriptase polymer chain reaction(RT-PCR) was conducted to confirm mRNA expression of NF and NSE in BMSCs co-cultured with SCs. Following co-culture time with SCs, BMSCs underwent morphological changes similar to those of cultured SCs and stained positively for GFAP, P75 and NF protein. Electrophoresis analysis revealed that the levels of NF and NSE on BMSC co-cultured with SCs related with other group maintained up to 21 days. Our results showed that BMSCs able to differentiate into SC-like cells using co-culture system in vitro. These findings could be helpful for the development of the cell-based therapeutic strategies for CNS repair.
DBP 스폰지와 DBP/PLGA 지지체에서의 인간 디스크세포 거동분석 비교
이선경(Seon Kyoung Lee),홍희경(Hee Kyung Hong),김수진(Su Jin Kim),김용기(Yong Ki Kim),송이슬(Yi Seul Song),하윤(Yoon Ha),이동원(Dong Won Lee),강길선(Gil Son Khang) 한국고분자학회 2010 폴리머 Vol.34 No.5
본 연구팀은 DBP를 함침시킨 물성이 서로 다른 스폰지와 PLGA 지지체를 제작한 후 세포 부착, 증식 및 형태 유지를 알아보기 위한 실험을 수행하였다. WST 분석법과 SEM 관찰을 통하여 스폰지에 비해서 PLGA 지지체에서의 세포의 증식이 활발한 것을 확인하였고, RT-PCR을 통해 디스크세포에서 특이적으로 발현하는 제2형 콜라겐과 어그리칸의 발현을 확인하였다. WST 결과, 세포 증식률은 DBP/PLGA 지지체가 DBP를 함침시킨 스폰지보다 세포 증식률이 높음을 확인하였다. 본 연구팀은 스폰지보다 PLGA 지지체가 인간디스크의 표현형 유지 및 중식에 있어서 긍정적인 영향을 미치는 것을 확인하였다. We fabricated sponge and poly(lactide-co-glycolide) (PLGA) scaffolds impregnated demi-neralized bone particle(DBP) (DBP/PLGA) and investigated proper condition to proliferation and phenotype maintenance of intervertebral disc(IVD) cells by comparison between DBP/PLGA scaffold and DBP sponge. DBP/PIGGA scaffolds were prepared by solvent casting/salt leaching. Human IVD cells were seeded in scaffolds of two types. Cell viability and proliferation according to scaffolds were analyzed by WST assay and SEM. RT-PCR was assessed to measure mRNA expression of aggrecan and type Ⅱ collagen of human IVD cells. In WST assay results, cell viability in scaffolds impregnated DBP/PLGA scaffold were higher than DBP sponge. We could observe that disc cell mRNA expressed better in DBP/PLGA scaffold than DBP sponge. We concluded that the using of DBP/PLGA in terms of scaffold fabrication for bio-disc with human IVD cells is helpful growth of disc cells maintenance of phenotypes.
노인과 젊은 성인의 발목발등굽힘 관절가동범위와 동적 균형 상관성 대한 이해
서해용(Hae-yong Seo),한지혜(Ji-hye Han),김민주(Min-ju Kim),김아연(Ah-yeon Kim),송이슬(Yi-seul Song),김수진(Su-jin Kim) 한국전문물리치료학회 2018 한국전문물리치료학회지 Vol.25 No.2
Background: Deficits of both ankle dorsiflexion range of motion (DFROM) and dynamic balance are shown in persons with chronic ankle instability and the elderly, with the risk of falls. Objects: This study aims to investigate the relationship between DFROM and dynamic balance in elderly subjects and young adults. Methods: Fifty-nine subjects were divided into three groups: ankle stability young group (SY), ankle instability young group (IY) and ankle stability older group (SO). We recruited three old subjects with ankle instability, but excluded them during a pilot testing due to the safety issue. DFROM was measured by weight bearing lunge test (WBLT) and dynamic balance was measured via star excursion balance test (SEBT) in anteromedial, medial, and posteromedial directions. The group differences in WBLT and SEBT and each group’s correlation between WBLT and SEBT were detected using the R statistical software package. Results: The dorsiflexion range of motion was significantly different between the SY, IY, and SO groups. The SO group showed the highest DFROM and IY group showed the lowest DFROM (SY: 45.88±.66 ˚ , IY: 39.53±1.63 ˚ , SO: 47.94±.50 ˚ ; p<.001). However, the SO group showed the lowest dynamic balance score for all SEBT directions (SY: 87.24±2.05 ㎝, IY: 83.20±1.30 ㎝, SO: 77.23±2.07 ㎝; p<.05) and there was no relationship between the dorsiflexion range of motion and dynamic balance in any group. Conclusion: Our findings suggest that ankle DFROM is not a crucial factor for dynamic stability regardless of aging and ankle instability. Other factors such as muscle strength or movement coordination should be considered for training dynamic balance. Therefore, we need to establish the rehabilitation process by measuring and treating ROM, balance, and muscle strength when treating young adults with and without ankle instability as well as elderly people.