http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
슈반세포와 골수간엽줄기세포의 공배양이 골수간엽줄기세포의 분화와 증식에 미치는 영향(2)
김초민 ( Cho Min Kim ),김순희 ( Soon Hee Kim ),송이슬 ( Yi Seul Song ),정수현 ( Su Hyun Jung ),박종학 ( Jong Hak Park ),오재민 ( Jae Min Oh ),이동원 ( Dong Won Lee ),유일수 ( Il Sou Yoo ),이종문 ( John M Rhee ),강길선 ( Gil Son Kh 한국조직공학·재생의학회 2009 조직공학과 재생의학 Vol.6 No.4
Schwann cell(SC) play an important role as they are the source of different trophic substances and present a great neurotrophic activity. Bone marrow stromal cells(BMSCs) contain mesenchymal stem cell and progenitor cells. BMSCs can differentiate into various cell types, including muscle and brain cell. The aim of this study is to investigate the differentiation of BMSCs through co-culture system with SCs. Pure SCs and BMSCs were cultured as control group. Expression of GFAP, P75 and NF were determined by immunocytochemical staining and reverse transcriptase polymer chain reaction(RT-PCR) was conducted to confirm mRNA expression of NF and NSE in BMSCs co-cultured with SCs. Following co-culture time with SCs, BMSCs underwent morphological changes similar to those of cultured SCs and stained positively for GFAP, P75 and NF protein. Electrophoresis analysis revealed that the levels of NF and NSE on BMSC co-cultured with SCs related with other group maintained up to 21 days. Our results showed that BMSCs able to differentiate into SC-like cells using co-culture system in vitro. These findings could be helpful for the development of the cell-based therapeutic strategies for CNS repair.