免役不全에 合倂한 感染症에 대한 抗菌劑의 使用法

成仁華 藥業新聞社 1985 (月刊) 醫藥情報 Vol.1985 No.10

당첨가가 한탄바이러스백신의 안정성에 미치는 영향

성인화 대한바이러스학회 1996 Journal of Bacteriology and Virology Vol.26 No.2

Hantaan virus vaccine was developed in 1988 and proved effective. This vaccine is a kind of inactivated vaccine, stable for two years when stored at 2-8 C. Almost virus vaccines including Hantaan virus vaccine are produced and kept in fluid state, and the immumogenicity can be easily destroyed at room temperature or at higher temperature. Therefore the vaccines should be kept in the refrigerator to maintain the immunogenicity. In this study, glucose and/or lactose was added as a stabilizer into Hantaan virus vaccine to increase the stability and dried in vaccum with ethanol treatment. 5% glucose and or lactose in Hantaan virus vaccine most effectively increased the stability of vaccine and maintained the immunogenicity at least for three months at room temperature. But drying with ethanol treatment did not help increasing the stability. These results suggest that glucose and lactose could be good stabilizer of virus vaccines.

한탄바이러스와 서울바이러스에 의한 신증후출혈열의 혈구응집저지 항체검사법을 이용한 혈청학적 감별진단

성인화,송기준,박대원,이호왕 대한바이러스학회 1986 Journal of Bacteriology and Virology Vol.16 No.2

Sucrose - Acetone 처리한 한탄바이러스 백신의 안정성

성인화 대한바이러스학회 1994 Journal of Bacteriology and Virology Vol.24 No.2

More then 1,000 patients suffered from hemorrhagic fever with renal syndrome are reported snnually in Korea and 50,000 to 100,000 patients in china, but the effective therapeutic agents and vaccines had not been developed. In 1988, Hantaan virus vaccine was developed and proved effective. This vaccine is a inactivated vaccine, stable for 24 months when stored at 2 8C. In the study, hantaan virus vaccine was treated with sucrose and acetone to make powdered vaccine, and the stability of sucrose-acetone treated vaccine was tested. Immunogenicity of Hantaan virus vaccine containing sucrose in low concentration was main- tained in some degree even after acetone treatment and exposure at room temperature for three months. This results suggest that sucrose could increase the stability of Hantaan virus vaccine and period of storage of Hantaan virus vaccine could be prolonged by adding sucrose and powdering.

서당을 첨가한 간염 B 바이러스 백신의 안정성

성인화 대한바이러스학회 1995 Journal of Bacteriology and Virology Vol.25 No.2

Active immunization against Hepatitis B virus in humans was initially invested by Krugman and collegues between 1971 and 1973 using a crude immunogenic preparation of Hepatitis B virus-containing serum by heat-inactivatiion. These studies led to the development of more sophisticated Hepatitis B vaccines harvested from the plasma of healthy human carriers. But adverse reactions were reported after inoculation of plasma-derived Hepatitis B vaccines. To overcome these problems, vaccine manufactures turned to recombinant DNA technology. Current licensed vaccines of Hepatitis B surface antigen are produced in large fermentation cultures of Saccharomyces cerevisiae yeast cells which carry a recombinant expression vector and plasmid DNA from E. coli. Nearly all the Hepatitis B vaccines manufactured in the world should be stored in the refrigerator to keep the immunogenicity of vaccines for a long time and without refrigeration, vaccines easily lose their immunogenicity. In this studies, as a step to develop the methods of increasing the stability of Hepatitis B vaccine which can be stored for a long period at room temperature or higher temperature conditions, sucrose was added into the vaccines and kept at different temperature conditions. Then the efficacies of vaccines were tested by inoculations of those vaccines into mice and titers of antibody against the surface antigen of Hepatitis B virus were evaluated. The results suggest sucrose could in some degree increase the stability of Hepatits B vaccine.

신증후 출혈열환자 뇨 침사내 세포에서의 한탄바이러스 항원 증명

성인화 대한바이러스학회 1992 Journal of Bacteriology and Virology Vol.22 No.2

Urine samples were collected from 10 patients diagnosed as hemorrhagic fever with renal syndrome(HFRS) by indirect immunofluorescent antibody test and 3 patients suspected as HFRS but confirmed as not infected by Hantaan virus. Cells in the urine sediments were examined to demonstrate the Hantaan virus antigens by indirect immunofluorescent antibody technique. The following results were obtained: 1. Cells in the urine sediments from 10 HFRS patients collected from day 7 to 40 after onset of HFRS showed Hantaan virus-specific fluorescent spot in their cytoplasms. 2. In samples from 3 suspected patients finally diagnosed as non-HFRS, Hantaan virus-specific antigens could not be found.

한탄바이러스를 접종한 쥐의 임파절 , 흉선 및 골수에서 한탄바이러스 항원의 증명

성인화 대한바이러스학회 1991 Journal of Bacteriology and Virology Vol.21 No.1

Lymph nodes, thymuses and bone marrows from SD rats inoculated with Hantaan virus were collected on 1, 2, 4, 6, 8, 10, 12, 20, 30 and 40 days after inoculation of Hantaan virus, and examined by indirect immunoluorescent antibody technique using monoclonal and polyclonal antibodies against Hantaan virus antigens to demonstrate the replication of Hantaan virus in centraI and peripheral immune organs. The results were summarized as follows. 1. Hantaan virus antigens were found in all the thymuses and bone marrows from the day 1 to 40 after inoculation of virus. 2. In the lymph nodes, antigens were found in different stage of infection according to the kinds of lymph node. Antigens were demonstrated on the day 30 and 40 in submandibular nodes, from the day 14 to 40 in parabronchial nodes, from the day 6 to 40 in rnesenteric nodes, and only on the day 14 in iliac nodes. 3. There were no differences of the results between using monoclonal antibody and polyclonal antibody against Hantaan virus antigens. 4. Hantaan virus antigens were not found in tissues from normal SD rats. 5. Identification of cells which had Hantaan virus antigens were not possible under the fluorescent microscope.

당첨가가 B형 간염 바이러스 백신의 안정성에 미치는 영향

성인화 대한바이러스학회 1997 Journal of Bacteriology and Virology Vol.27 No.2

신증후출혈열 환자 소변의 한탄바이러스에 대한 항체가와 혈청중 BUN 및 Creatinine치의 변화

성인화 대한바이러스학회 1990 Journal of Bacteriology and Virology Vol.20 No.1

백두옹에서 분리한 Linoleic acid와 시약용 Linoleic acid의 Methicillin-Resistant Staphylococcus aureus와 Candida albicans에 대한 항균효과 비교

성인화 대한화학요법학회 2002 대한화학요법학회지 Vol.20 No.4

목적 : 항생제 내성균들의 등장으로 이에 대처할 새로운 항생제의 개발이 요구되고 있는 상황에서 천연 항균제를 개발하기 위한 연구의 일환으로 전 연구에서 할미꽃의 뿌리인 백두옹으로부터 분리한 linoleic acid와 시판 중인 시약용 linoleic acid의 MRSA와 Candida albicans에 대한 항균활성의 차이를 규명하는 것이다. 방법 : 두 가지 linoleic acids의 항균활성을 비교하기 위하여 MRSAs는 Mueller Hinton 배지를, Candida albicans는 Sabouraud 배지를 사용하여 disk diffusion법으로 항균검사를 시행하였다. 결과 : 환자에서 분리된 MRSA 10주와 포도상구균 표준 균주 (ATCC 65389)에 대하여 백두옹에서 추출된 linoleic acid와 시약용 linoleic acid가 거의 비슷한 항균활성을 나타내었으나, 환자에서 분리된 Candida albicans 10주와 Candida albicans 표준 균주(ATCC 10231)에 대하여는 백두옹에서 분리된 linoleic acid 만이 항진균 활성을 나타내었다. 결론 : 할미꽃 뿌리인 백두옹에서 분리된 linoleic acid와 시약용으로 정제된 linoleic acid가 MRSA에 대하여 서로 비슷한 항균활성을 나타내었으나 Candida albicans에 대하여는 백두옹에서 분리된 linoleic acid 만이 항진균활성을 나타내었은데 그 이유는 현재는 알 수 없고 앞으로의 연구를 통하여 밝혀지기를 기대한다. Background : Recently advent of MRSA with reduced susceptibility to vancomycin reported in Japan and the United States of America dismayed the whole people in the world. To cope with the antibiotic-resistant microorganisms effectively, developing new antimicrobial agents is required urgently. There has been a report that the linoleic acid isolated from the roots of Pulsatilla koreana NAKAI had antimicrobial activity. In this study, antimicrobial activity of commercial linoleic acid against MRSAs and Candida albicans was compared with that of the isolated linoleic acid isolated from Pulsatilla koreana. Methods : Antimicrobial activity and MIC of linoleic acids were evaluated by agar diffusion method using Mueller Hinton agar for 10 strains of MRSA and Staphylococcus aureus (ATCC 65389), Sabouraud's dextrose agar for Candida albicans (ATCC 10231) and 10 strains of Candida albicans. Results : Both isolated linoleic acid and reagent linoleic acid showed similar antimicrobial activities against 10 strains of MRSA and Staphylococcus aureus (ATCC 65389) and MIC was 0.5㎎/m(L The isolated linoleic acid revealed antifungal activity against all the stains of Candida albicans, but commercial linoleic acid did not showed any antifungal activity against all the stains of Candida albicans. Conclusion : Linoleic acid isolated from the roots of Pulsatilla koreana and commercial linoleic acid showed antimicrobial activities agsinst MRSA, but against Candida albicans only linoleic acid isolated from the roots of Pulsatilla koreana. revealed antifungal activity and commercial linoleic acid did not showed antifugal activity.