미국 하와이주 주민 및 쥐의 Hantaan virus 감염에 대한 혈청 역학적 조사

이호왕,주용규,이상언,Arwin Diwan 대한바이러스학회 1987 Journal of Bacteriology and Virology Vol.17 No.2

Korean hemorrhagic fever was known to the world for the first time during the Korean War and its causative agent, Hantaan virus was discovered by Lee and Lee in 1976. Following this discovery, it became apparent that similar diseases such as Hemorrhagic nephrosonephritis in the Soviet Union, Nephropathia epidemica in Scandinavia, Epidemic hemorrhagic fever in China and Japan and Epidemic nephritis in Eastern Europe are caused by Hantaan and related viruses. Therefore in 1982, WHO recornmanded that the above mentioned diseases with different names should be refered to as Haemorrhagic fever with renal syndrome (HFRS). Recent seroepidemiologic surveys show that closely related agents antigenically similar to Hantaan virus are widely distributed in the world to a much greater geographic extent than previously recognized. This study was to prove the existence of Hantaan or related virus infection among the residents and the rats in the Hawaiian islands that is located in the center of the Pacific Ocean and the results are surnmarized as follows: 1. Rattus rattus, Rattus norvegicus, and Rattus exulans had antibodies against Hantaan yirus in the proportion of 84 to 1,159 (7.2%), 30 to 169 (17%), and 12 to 202 (5.9%) respectively. 2. Among 216 healthy Hawaiians, 4 persons had antibodies (1.9%). However, prevalence of antibody to Hantaan virus among Vector Control Personnel worked in Hawaii State Department of Health was 30.6% (11/36). 3. Hantaan virus antigen was not demonstrable in the lung tissues of antibody positive rats. 4. Up to the present tirne, there was no patient ever diagnosed as Haemorrhagic fever with renal syndrome clinically in the Hawaiian islands. The results of this study indicate that Hantaan virus or similar virus exists in the Hawaiian islands and further study is needed to demonstrate the occurrence of HFRS patient and to isolate Hantavirus in this area.

서울시내 각종연구수 동물실내 흰쥐의 Hantavirus감염조사

이호왕,백낙주,김효덕 대한바이러스학회 1986 Journal of Bacteriology and Virology Vol.16 No.2

Hantaan virus was first isolated in 1976 from field mice, Apodemus agrarius and in 1978 from the patient of Korean hemorrhagic fever in Korea by Lee and Lee. Hemmorragic fever with renal syndrome is a major public health problem throughout most of European and Asian land mass. Although predominantly associated with rural area, it is now being reco- gnized as an urban problem and as a particular hazard to laboratory staff using rodents for biomedical research. Hantavirus is a new Genus name which includes Hantaan virus, Seoul virus, Prospect Hill virus and Puumala virus in Byunyaviridae. Since 1976 there were outbreaks of Hemorrhagic fever with renal syndrome among personnel of animal rooms of medical centers in Korea and in Japan wehere Hantavirus experiments were never done, laboratory rat infection with Hantavirus in animal rooms of six different institutes in Seoul was studied. The results are as follows: 1. The Hantaan viral antigen were demonstrated in the pulmonary tissues of 41 (22.9%) rats out of 179 laboratory rats tested. 2. The immunofluorescent antibodies against Hantaan virus were demonstrated in 64.6% (126) of sera from 195 rats in the animal rooms of six different institutes. 3. The high levels of immunofluorescent antibodies reactive with Hantaan virus were detected in 26.7% (12) of sera from 45 personnel of animal rooms of six institutes. Although all of 12 persons developed symptoms of Hemorrhagic fever with renal syndrome, they were diagnosed as influenza by clinical physicians. 4. There was no difference in susceptibility of four kinds of laboratory rats to Hantaan virus. The above results indicated for the first time that several kinds of infected laboratory rats were reservoir of Hantavirus in the animal rooms of institutes in seoul.

한국주둔 미국군인과 서울시내 미국군사 시설내 집쥐의 Hantaan virus감염조사

이호왕,백낙주,주용규,구인회 대한바이러스학회 1986 Journal of Bacteriology and Virology Vol.16 No.2

인천 집쥐에서 한탄바이러스와 유산한 바이러스의 분리

이호왕,백낙주,주용규,강병남 대한바이러스학회 1986 Journal of Bacteriology and Virology Vol.16 No.2

Korean haemorrhagic fever (KHF) was recognized for the first time in Korea in 1951 during Korean War among United Nations troops in the central front although similar diseases to KHF had been reported as Haemorrhagic nephrosonephritis in Russia, as Epidemic haemorrhagic fever in China, as Nephropathia epidemica in Scandinavia and as Epidemic haemorrhagic fever in eastern Europe and in Japan. In 1976 Lee and Lee discovered the etiologic agent of KHF in the lungs of the striped field mouse, Apodemus agrarius, which gave specific immunofluorescent reaction with sera from patients convalescent from KHF for the first time in Korea. In 1978 Lee et al. Had isolated the same agent from KHF patients sera and had succeeded the propagation of the virus in Apodemus agrarius, and it was named Hantaan virus after Hantaan river. Natural reservoirs of Haemorrhagic fever with renal syndrome are not only field mice and laboratory rats but also wild urban rats. Therefore, we have collected the wild urban rats in the international port of Incheon for serologic survey of Hantaan virus infection and carried out isolation of Hantaan-like virus from infected rats. 1. Among the 215 wild urban rats collected in the international port of Incheon, 112 (52%) had antibodies against Hantaan virus and 51(24%) had Hantaan virus-like antigen. 2. Fourteen strains of Hantaan-like virus were isolated from S.D. rats inculated with the suspension of lungs from 60 wild urban rats. One strain of Hantaan-like virus was isolated from S. D. rats inoculated with the antigen negative lung tissues from 10 urban rats that contained antibodies in the sera against Hantaan virus. In positive urban rats, the largest amount of Hantaan-like virus antigen was distributed in lungs, followed by spleen, liver and kidneys, in that order. 3. 82-3 strain isolated from urban rats in Incheon was cultured in Vero E6 cells, and this virus had antigenicities of both Hantaan and Punmala viruses which cause haemorrhagic fevers in Korea and Scandinavia by immunofluorescent antibody technique. Further study to characterize this Hantaan-like virus is in progress.

한국 , 일본 , 홍콩 가축의 한탄바이러스에 대한 형광항체 분포

이호왕,이광평 대한바이러스학회 1989 Journal of Bacteriology and Virology Vol.19 No.1

실험실 감염의 문제점

이호왕 大韓法醫學會 1985 대한법의학회지 Vol.9 No.1-2

신증후 출혈열환자의 Immunoglobulin 형성에 관한 연구

이호왕,성인화,오인석,주용규 대한바이러스학회 1987 Journal of Bacteriology and Virology Vol.17 No.1

This experiment investigated 5 classes of immunoglobulins to Hantaan virus in patients of Hemorrhagic fever with renal syndrome from immediately after onset of the disease to 2 months. The results obtained from this experiment were as follows: 1. The highest titer of IgG antibody to Hantaan virus could be demonstrated and reached to a peak between 2nd and 3rd week from onset of fever. Most of the patients showed high titers of immunofluorescent antibody between 4,096 and 16,384. 2. IgA antibody to Hantaan virus in patients of Hemorrhagic fever with renal syndrome was relatively high and reached a peak between 2nd and 3rd week from onset of the disease, and it was maintained with high titer of antibody for 2 months. Peak titers of immunofluorescent antibody were between 1,024 and 4,096. 3. IgM antibody to Hantaan virus in the patients of Hemmorrhagic fever with renal syndrome was demonstrated in all of the patients after onset of the disease and the titers of the antibody were between 256 and 4,096 for 2 months. 4. Though IgD antibody in patients of Hemorrhagic fever with renal syndrome was reached to a peak between 2nd and 3rd weeks after onset of the disease, the titers of the antibody were within 1,024 and it was demonstrated for 2 months. 5. IgE antibody in the patients of Hemorrhagic fever with renal syndrome was irregularly appeared and the increase of antibody was demonstrated in half of the patients. Above results revealed that the demonstration of IgM could be the important criteria in diagnosis of the primary infection with Hantaan virus and that the significance of increase of IgD antibodies to Hantaan virus dur- ing illness remains to be analysed.

신증후출혈열환자의 한탄 및 서울바이러스에 대한 중화항체 형성

이호왕,이평우,김만중 대한바이러스학회 1987 Journal of Bacteriology and Virology Vol.17 No.1

The production of neutralizing antibodies against Hantaan and Seoul viruses in sera of five patients diagnosed as typical Korean hemorrhagic fever was investigated using plaque reduction neutralization test. Cross reactivity of sera of the patients to Hantaan and Seoul viruses which are related but distinct antigenically was also studied. The patients with Korean hemorrhagic fever have been bled at weekly intervals from 3 days to 66 days after the onset of illness and sera were prepared and stored in -65C until use. Neutralizing antibody titers against Hantaan and Seoul viruses were determined by a serum dilution plaque reduction neutralization (PRN) method employing immunoperoxidase staining in Vero E6 cells. In gerneral, the antibodies were detected as early as 3 days after the onset of disease. The peak titers were reached at around 2 weeks after the onset of illness and the antibodies were persisted up to 66 days although the titers were decreased slowly. All sera used from the patients were neutralized Hantaan viruses in higher titers in contrast with lower titers with Seoul viruses. It can be concluded that HFRS patients in this study were infected with Hantaan virus in rural areas of Korea.

면역점착적혈구응집반응에 의한 신증후출혈열환자 혈청중 한탄 및 서울바이러스에 대한 항체형성

이호왕,이평우,김홍래 대한바이러스학회 1987 Journal of Bacteriology and Virology Vol.17 No.1

Immune adherence hemagglutination (IAHA) test was used to investigate the production of antibodies against Hantaan (HTN/83-138) and Seoul (SEO/80-39) viruses in sera from Hemorrhagic fever with renal syndrome (HFRS) patients who were infected with Hantaan virus in Korea. The five patients diagnosed as typical HFRS have been bled at weekly intervals starting from 3 days after onset of disease up to 66 days. Sera were tested to determine the titers of antibodies as well as the reactivities against Hantaan and Seoul viral antigens. The results are as follows; 1. IAHA antibodies against Hantaan viral antigen: The antibodies increased 3 days after the onset of disease and reached at peak around one week later. The antibody titers were risen rapidly and the maximum titers were reached at around 2 weeks after onset of the disease and persisted for 2-3 weeks. Thereafter the titers were decreased slowly. 2. IAHA antibodies against Seoul viral antigen: The antibodies were detectable 3 days after the onset of disease and the titers of antibody were reached peak at around one week later and persisted with same level for 2 months. However the titers of antibody were not as high as Hantaan viral antigen. 3. Reactivities of HFRS sera against Hantaan and Seoul viral antigens: The reactivity of Hantaan viral antigen was always 4-16 times higher than Seoul viral antigen in all sera tested except the sera from very early stage of illness. The clear differences in reactivity between Hantaan and Seoul viral antigens were observed in all sera used regardless of time of bleeding.

신증후출혈열환자의 Hantaan Virus , Seoul Virus 및 Puumala Virus에 대한 형광항체에 관한 연구

이호왕,주용규,두창대,백낙주 대한바이러스학회 1987 Journal of Bacteriology and Virology Vol.17 No.1

Five patients with Hemorrhagic fever with renal syndrome who were infected with Hantaan virus were bled at one week interval for two months after illness and the sera were used for current study. Serum antibody titers against Hantaan, Seoul and Puumala viruses were tested and compared by indirect immunofluorescent antibody (IFA) technique. The results are as follows. 1. Immunofluorescent (IF) antibodies against Hantaan virus were maximal about 2-3 weeks after the onset of illness and peak reciprocal IF antibody titers were 1,024-16,384. The titers of IF antibody against Seoul virus were lower in two folds than those of Hantaan virus. 2. IF antibodies against Puumala virus were remarkablely lower. Peak IF antibody titers were attained about 2-3 weeks following the onset of illness and the reciprocal titer was 128 at the time. 3. IgM antibodies in the sera of patients were only detectable against Hantaan and Seoul viruses with same titers. Peak IF IgM antibody titers were attained about one week following the onset of illness and then decreased rapidly after three weeks. This study concluded that infections with Hantaan and Seoul viruses were not differentiated by the demonstration of IgG antibody titers but infection with Puumala virus was easily differentiated. Also, the demonstration of IgM antibody was the clue of recent infection of Hantaan or Seoul virus.