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식이중의 동물성 및 식물성단백질의 종류와 배합비율에 따른 흰쥐 (Rat)와 흰생쥐 (Mouse)의 성장과 혈액성분에 관한 비교연구
선영실(Young Sil Sun),이성우(Sung Woo Lee),한인규(In Kyu Han) 한국식품영양과학회 1983 한국식품영양과학회지 Vol.12 No.3
흰쥐와 흰생쥐 암ㆍ수 각 30마리씩에 대한 실험개시기와 종료후의 결과로서 나타난 식이에 대한 사양실험, 대사실험, 혈액성분분석 실시 결과는 다음과 같다.<br/> 1. 증체량은 흰쥐 암ㆍ수 모두 동물성단백질 배합비율이 높아질수록 증가했다. 흰생쥐는 숫생쥐가 C식이에서 최대를, E식이에서 최저를 나타냈으나 암생쥐는 반대였다.<br/> 2. 식이효율은 흰쥐 암ㆍ수 모두 동물성 단백질 배합비율이 높아질수록 증가했으며 암쥐보다 숫쥐에서 높았다. 흰생쥐는 숫생쥐가 증체량과 같은 경향이었으나 암생쥐는 일정경향이 없었다. 암생쥐보다 숫생쥐의 식이효율이 높았다.<br/> 3. 단백질효율은 흰쥐 암ㆍ수 모두 동물성단백질 배합비율이 높아질수록 증가했다. 흰생쥐는 숫생쥐의 증체량, 식이효율은 같은 경향이나 암생쥐는 일정한 경향이 없었다.<br/> 4. 질소보유율은 흰쥐 암ㆍ수 모두 단백질수준이 같을때 섭취질소량에 비례함을 보였다. 흰생쥐는 숫생쥐가 동물성 배합비율이 높아질때 증가하였다. 암생쥐는 일정한 경향이 없었다. 질소축적율은 흰생쥐에서 높게 나타나 체내 단백질이용이 흰쥐보다 높음을 알 수 있었다.<br/> 5. 혈청성분에서는 혈청 총단백질량은 흰쥐 암ㆍ수 모두 차이가 없으나 동물성단백질 배합비율이높아질수록 증가하는 경향이었다. 흰생쥐는 암ㆍ수 모두 일정한 경향이 없었다. 혈청요소질소량은 흰쥐의 경우 암ㆍ수 모두 식물성단백질 배합비율이 높아질수록 증가했다. 흰생쥐는 알정한 경향이 없었다. 혈청콜레스테롤량은 흰쥐 암ㆍ수 모두 단백질배합비율에 관계없이 일정한 경향이 없었다. 흰생쥐는 숫생쥐가 식물성단백질 배합비율이 높아질수록 증가했으나 암생쥐는 일정한 경향이 없었다.<br/> 6. 위의 결과로 실험식이중의 동물성단백질 배합비율이 높은 A, B식이에서 흰쥐는 증체량, 사료효율, 단백질효율이 좋았다. 흰생쥐는 숫생쥐가 C식이, 암생쥐가 E식이에서 높게 나타났다. 이는 생쥐의 증체량이 이유에서부터 성숙기까지 큰 차이가 없다는 것과 흰쥐와는 다른 단백질수준이 요구된다고 생각된다. In order to investigate the effect of the combined dietary animal and vegetable proteins on growing female and male rats and mice. 25 female and 25 male rats of 4 weeks old weighing approximately 43-65 g and 47-60g, respectively, were subjected to feeding trials for 4 weeks and then subsequently to metabolic trials for 10 days. In case of mice, 25 female and 25 male mice of 4 weeks old weighing approximately 12-17g and 12-16g, respectively, were equally treated.<br/> The dietary energy level was set as 3600 kcal ME/㎏, and protein and fat provided respectively 12 and 5% of the dietary energy. The rest 83% of the energy level was supplied with appropriate amounts of starch and glucose. The following results were obtained. The body weight gain of female and male rats were increased as the combined dietary animal protein level increased. Whereas that of male mice was the highest for the diet E (Ap 10+Vp90). Food efficiencies both of female and male rats were improved as combined dietary of animal protein increased. Male rats were superior to that of female. In the mice, the food efficiency value of male mice was superior to that of female. And the values of the male showed the same fashion as that in the growth rate, although it was not for the female. Protein efficiencies, both of female and male rats, were improved as combined dietary of animal protein level increased. That of male mice was in accord with the body weight gain and food efficiencies. But that of female mice was not accordant. Nitrogen retention of the experimental diets in the same protein level was in proportion to nitrogen intake. That of male mice was improved as animal protein level increased, but, that of female mice was not. Body protein utilizability was found to be superior in mice to rats. Blood serum protein level was not found to be affected by the experimental diets in the same protein level. The best combination ratio of animal and vegetable protein seems to be variable depending on the sex and species of animals, and the best diets for female and male rats and female and male mice were found to be experimental diet A(Ap 10+Vp 90) or diet B(Ap 75+Vp 25), diet C(Ap 50+Vp 50) and diet E(Ap 10+Vp 90), respectively. From the above-mentioned results that there was no difference in growth rate or body weight gain from the weanling period to puberty, and that the protein requirements of rats and mice were different from each other.
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Guar gum의 섭취 수준과 기간이 흰쥐의 영양소 흡수율에 미치는 영향
김상연,선영실,장유경 漢陽大學校 韓國生活科學硏究所 1993 韓國 生活 科學 硏究 Vol.- No.11
This study was performed to investigate apparent absorption of nutrients according to level and duration of guar gum intake. Eighteen male rats of Sparague-Dawley strain weighing 50g were blocked into three groups : FF, 5G, 10G(Fiber Free, 5% Guar gam, 10% Guar gum). Balance studies were conducted at 2nd week, 4th week and 8th week. Feed in-take body weight gains, apparent absorption of protein, fat, Ca and P were measured. The results obtained in this study were summarized as follows : (1) Feed intake was not significantly different among the groups during each balance studies. Weiht gain was decreased according to level of guar gum intake during each balance studies. (2) Protein apparent absorption of 5G were not significantly different with that of 10G at 2nd week and 4th week but at 8th week protein apparent absorption of 10G was singificantly lower than that of 5G According to duration of guar gum intake, protein apparent absorption of FF and 5G were not significantly different. Wherease protein apparent absorption of 10G was significantly decreased at 8th week. (3) Fat and Ca apparent absorption were not significantly different among groups at 2nd week. Fat apparent absorption of 10G was not significantly lower than that of 5G at 4th week and 8th week. According to duration of guar gum intake, fat and Ca apparent absorption of FF and 5G were not significant different. But fat and Ca apparent absorption of 10G was significantly decreased according to duration of fur gum intake. (4) Phosphorus apparent absorption were not significantly different among groups at 2, 4th week. But phosphorus apparent absorption of 10G were significantly higher than that of 5G at 8th week. According to duration of guar gum intake, phosphorus apparent absorption of FF and 10G were not influenced. But Phosphorus apparent absorption of 5G was significantly decreased according to duration of guar gum intake. From the results of this study, we could recommend 5% guar gum in diet of obese man and di abetics.
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