미생물 생장 예측모델과 반응표면분석법을 이용한 Vibrio parahaemolyticus의 신속 증균배지 개발

서연희,이소영,김은지,오세욱,Yeon-Hee Seo,So-Young Lee,Unji Kim,Se-Wook Oh 한국식품위생안전성학회 2023 한국식품위생안전성학회지 Vol.38 No.6

In this study, we developed Rapid Enrichment Broth for Vibrio parahaemolyticus (REB-V), a broth capable enriching V. parahaemolyticus from 10⁰ CFU/mL to 10⁶ CFU/mL within 6 hours, which greatly facilitates the rapid detection of V. parahaemolyticus. Using a modified Gompertz model and response surface methodology, we optimized supplement sources to rapidly enrich V. parahaemolyticus. The addition of 0.003 g/10 mL of D-(+)-mannose, 0.002 g/10 mL of L-valine, and 0.002 g/10 mL of magnesium sulfate to 2% (w/v) NaCl BPW was the most effective combination of V. parahaemolyticus enrichment. Optimal V. parahaemolyticus culture conditions using REB-V were at pH 7.84 and 37℃. To confirm REB-V culture efficiency compared to 2% (w/v) NaCl BPW, we assessed the amount of enrichment achieved in 7 hours in each medium and extracted DNA samples from each culture every hour. Real-time PCR was performed using the extracted DNA to verify the applicability of this REB-V culture method to molecular diagnosis. V. parahaemolyticus was enriched to 5.452±0.151 Log CFU/mL in 2% (w/v) NaCl BPW in 7 hours, while in REB-V, it reached 7.831±0.323 Log CFU/mL. This confirmed that REB-V enriched V. parahaemolyticus to more than 10⁶ CFU/mL within 6 hours. The enrichment rate of REB-V was faster than that of 2% (w/v) NaCl BPW, and the amount of enrichment within the same time was greater than that of 2% (w/v) NaCl BPW, indicating that REB-V exhibits excellent enrichment efficiency. 본 논문은 적은 농도로 존재하는 Vibrio parahaemolyticus를 6시간이내에 10⁶ CFU/mL까지 신속하게 배양가능한 배지인 Rapid Enrichment Broth for V. parahaemolyticus(REB-V)를 개발하여 V. parahaemolyticus를 신속하게 검출할 수 있도록 하였다. Modified Gompertz model과 RSM를 활용하여 V. parahaemolyticus를 신속하게 증균할 수 있도록 첨가 성분을 최적화하였다. 그 결과 2%(w/v) NaCl BPW에 D-(+)-mannose 0.3 g/L, L-valine 0.2 g/L, magnesium sulfate 0.2 g/L를 첨가하였을 때 V. parahaemolyticus의 증균량이 최대였다. REB-V의 배양조건을 pH 7.84와 37℃으로 최적화하였으며, 2%(w/v) NaCl BPW와 비교하여 REB-V의 증균 효율을 확인하기 위해 7시간 증균을 통해 증균량을 평가하였다. 또한 매시간마다 배양액에서 DNA를 추출하였고, 추출한 DNA로 real-time PCR을 수행하여 REB-V의 분자진단법 적용가능성을 확인하였다. 그 결과 7시간 동안 2%(w/v) NaCl BPW에서 V. parahaemolyticus는 5.452±0.151 Log CFU/mL까지 증균되었고, REB-V에서는 7.831±0.323 Log CFU/mL의 V. parahaemolyticus가 증균되었다. 최종적으로 REB-V에서 6시간 이내에 10⁶ CFU/mL 이상으로 증균된 것을 확인하였고, REB-V의 증균속도가 2%(w/v) NaCl BPW보다 빠르고 같은 시간 내의 증균량이 2%(w/v) NaCl BPW보다 많았음을 통해 REB-V의 증균효율이 우수한 것으로 확인되었다.

체육전공 예비교사들의 교육실습 전 · 후에 나타난 교직관의 상대적 중요도 차이

서연희(Seo, Yeon-Hee) 한국사회체육학회 2017 한국사회체육학회지 Vol.0 No.68

This study is to analyze a difference in relative importance of teaching profession view shown before · after classroom teaching practice of pre-service teachers majoring in physical education. For this end, an AHP survey was conducted. with regard to AHP analysis, Methods: pre-service teachers at 7 colleges of education were selected according to region (1 college in Seoul, 2 in Chungnam, 1 in Chungbuk, 1 in Jeonnam and 2 in Jeonbuk). The results of analyzing pairwise comparison questionnaires for respective 164 pre-service teachers before · after student teaching are as follows. First, with regard to relative importance of teaching profession view before student teaching, it was shown that ‘positive teaching profession view (0.381)’ had the highest relative importance, being followed by ‘negative teaching profession view (0.381)’ and ‘social teaching profession view (0.218)’. And it was shown to be the lowest in ‘teaching profession aspiration (0.152)’. Second, it was shown that relative importance of teaching profession view shown after student teaching was the highest in ‘teaching profession aspiration (0.401)’, being followed by‘ positive teaching profession view (0.266)’ and ‘negative teaching profession view (0.184)’. And it was shown to be the lowest in ‘social teaching profession view (0.149)’. Third, with regard to a change in priority and relative importance of teaching profession view in pre-service teachers majoring in physical education before · after student teaching, positive teaching profession view factor weight was decreased by 0.115. And negative teaching profession view factor weight was decreased by 0.065. Social teaching profession view factor weight was decreased by 0.069. However, it was shown that teaching profession aspiration factor weight is increased by 0.249. the significance of hands-on experience through pre-service teachers’ teaching practice and the necessity of follow-up study to enhance pre-service teachers’ competency as teacher and promote professionalism was proposed.

간호대학생의 인공지능에 대한 지식, 인식 및 수용태도가 인공지능 이용의도에 미치는 영향

서연희(Yon Hee Seo),조경아(Kyong Ah Cho) 한국간호연구학회 2022 한국간호연구학회지 Vol.6 No.3

Purpose : Artificial intelligence (AI) has potentials to significantly transform a role of nurses and revolutionise practices of healthcare systems. The aim of this study is to identify influences of AI Knowledge, Perception, and Acceptance Attitude on nursing students’ Intentions to use AI-based healthcare technologies. Methods : The participants included 241 nursing students in Gyeonggi-do and Jeollanam-do, with data collected from 30 May to 30 June 2022 using self-reported questionnaires. The data were analyzed using the SPSS/WIN 25.0 program, with independent t-tests, one-way ANOVA, Pearson’s correlations, and multiple linear regression. Results : The results revealed that AI Knowledge positively correlated with AI perception(r=.40, p<.001), and Acceptance Attitude toward AI(r=.17, p=.007). AI perception positively correlated with AI Acceptance Attitude(r=.53, p <.001), and Intentions to use AI(r=.46, p<.001). AI Acceptance Attitude also positively correlated with Intentions to use AI(r=.52, p<.001). The factors influencing Intentions to use AI-based healthcare technologies included AI Perception(β=.30) and Acceptance Attitude AI Acceptance Attitude(β=.38). The adjusted R2 was .318. Conclusions : It is necessary to develop systematic educational programs on AI technologies and form an organizational culture to improve nursing clinical competency and professionalism for nursing students in healthcare setting.

국가대표 여자 체조선수들의 심리기술훈련 프로그램 적용에 따른 효과 검증

서연희(Yeon Hee Seo),박현조(Hyun Jo Park),강성구(Sung Goo Kang) 한국여성체육학회 2008 한국여성체육학회지 Vol.22 No.4

코로나19 상황에서 초등학교 신규교사의 직무경험에 관한 사례연구

서연희 ( Seo Yeonhee ),최준섭 ( Choi Joonsup ) 한국초등교육학회 2022 초등교육연구 Vol.35 No.4

This study was conducted to explore the work experiences of new elementary school teachers in the COVID-19 situation. The data analyzed in this study were the interview work of 8 elementary school teachers in public elementary schools in D metropolitan city, using the case study. As a result of analyzing the participants' interview according to the content analysis procedure, a total of 4 large categories, 14 subcategories, and 48 meanings were derived. The results of a study on the experiences of new elementary school teachers in the COVID-19 situation are as follows. Four major categories were derived: experience of a teaching life that began in confusion, contrary to expectations, experience of a lonely and difficult school life, experience of a strong will to positively overcome the school life in crisis, and experience of hope to overcome the crisis of COVID-19 and become growing teachers through various help. Based on the results of this study, discussions, conclusions, and suggestions were discussed. Based on the founding above, the experiences of new elementary school teachers facing the COVID-19 situation and teaching for the first time can be used as basic data to explore necessary support measures to understand their lives and adapt to the teaching profession.

한국어판 간호대학생의 인공지능에 대한 태도 측정도구 신뢰도 및 타당도 검증

서연희(Seo, Yon Hee),안정원(Ahn, Jung-Won) 한국간호교육학회 2022 한국간호교육학회지 Vol.28 No.4

초등학생의 체육수업 소외와 스트레스 관계

서연희(Yeon Hee Seo),이대형(Dae Hyung Lee),송지환(Ji Hwan Song) 한국체육교육학회 2011 한국체육교육학회지 Vol.16 No.1

본 연구는 초등학교 학생들이 경험한 체육수업소외와 체육수업스트레스의 변인간의 관계성을 알아보고, 체육수업 내실화에 기여하는데 목적이 있다. 본 연구를 위해 J시에 소재한 초등학생 6학년 아동 전체(남 127명, 여 153명)의 설문자료를 분석에 사용하였으며, 빈도분석(frequency analysis), 요인분석(factor analysis), 상관관계(correlation analysis), 중다회기분석(multiple regression analsis)의 통계적 검증을 통해 다음과 같은 결론을 도출하였다. 첫째, 초등학생들이 체육수업소외(체육교사소외, 운동능력소외, 교과내용소외)는 학습내용스트레스에 영향을 미치는 것으로 나타났다. 둘째, 체육수업소외(친구관계소외, 운동능력소외, 교과내용소외)는 친구들과의 마찰 스트레스에 영향을 미치는 것으로 나타났다. 셋째, 체육수업소외(운동능력소외, 친구관계소외)는 자신감부족 스트레스에 영향을 미치는 것으로 나타났다. 넷째, 체육수업소외(체육교사소외, 교과내용소외)는 교사불만 스트레스에 영향을 미치는 것으로 나타났다. 다섯째, 체육수업소외(운동능력소외, 체육교사소외, 친구관계소외, 교과내용소외)는 시험부담 스트레스에 영향을 미치는 것으로 통계적으로 유의한 결론이 나타났다. 이와 같은 결과는 초등학생들의 체육수업소외는 학습내용 스트레스, 친구들과 마찰 스트레스, 자신감부족 스트레스, 교사불만 스트레스, 시험부담 스트레스에 영향을 미치고 있음이 확인되었다. This study searches relation between fantast of physical education class estrangement and Cheyuksueopseuteureseu that primary school students recognize, and is tendencious to contribute at physical education class main room Tuesday. Used question data of J city locating elementary school pupil 6 class child whole (127 others, 153 women) in analysis for this study, and drew following conclusion through frequency assay, interrelation, Jungdahoegibunseok`s statistical verification. First, elementary school pupils appeared that physical education class estrangement (gymnast, motor ability, faith subject contents estrangement) affects to Hakseupnaeyongseuteureseu. Second, was expose that physical education class estrangement (friend relation, motor ability, subject contents) affects in attrition stress with friends. Third, was expose that physical education class estrangement (motor ability, friend relation) affects in pride tribe stress. Fourth, was expose that physical education class estrangement (gymnast estrangement, subject contents estrangement) affects in teacher complaint stress. Fifth, conclusion that keep in mind statistically that physical education class estrangement (motor ability, gymnast, friend relation, subject contents) affects in examination burden stress appeared.

간호대학생의 정신건강정보이해능력과 인권감수성이 정신장애인에 대한 사회적 거리감에 미치는 영향

서연희(Seo Yonhee),조경아(Cho Kyongah) 학습자중심교과교육학회 2020 학습자중심교과교육연구 Vol.20 No.21

본 연구는 간호대학생의 정신건강정보이해능력과 인권감수성이 정신장애인에 대한 사회적 거리감에 미치는 영향을 확인하고자 시도되었다. 연구대상자는 서울특별시와 경기도 소재의 5개 대학에 재학중인 간호대학생 208명을 모집하였으며, 자료수집은 2019년 5월 28일부터 6월 11까지 자기기입식 설문지를 이용하여 수집하였다. 본 연구결과, 정신건강정보이해능력은 16점 만점에 11.50점, 인권감수성은 75점 만점에 55.03점, 사회적 거리감은 40점 만점에 23.85점이었으며, 사회적 거리감에 영향을 미치는 요인으로 인권감수성(β=.301, p<.01), 정신질환 교육경험(β=306, p<.01), 정신건강정보이해능력(β=125, p=.05)으로 확인되어 전체 설명력은 19.5%로 나타났다. 이에 정신장애인에 대한 사회적 거리감을 완화시키기 위해서는 간호대학생의 정신건강정보이해능력과 인권감수성을 향상시키고 체계적인 교육 전략과 교육 프로그램을 개발하여 적용할 것을 제언한다. This study was to investigate the effects of mental health literacy and human rights sensitivity of nursing students on social distance in people with mental disorders. The participants comprised 208 nursing students from 5 universities located in Seoul and Gyeonggi-do. Data were collected using self-report questionnaires from May to June 2019. The data were analyzed using descriptive statistics, Independent t-tests, Pearson s correlation coefficient, and stepwise multiple regression. As a result of this study, mental health literacy was 11.50 out of 16, human rights sensitivity was 55.03 out of 75, and social distancing was 23.85 out of 40. The factors affecting social distance were human rights sensitivity (β=.301, p<.01), mental illness education experience (β=306, p<.01), and mental health literacy (β=125, p=.05); the explanatory power of the model was 19.5%. Therefore, in order to alleviate social distance towards people with mental disorders, it is necessary to improve mental health literacy and human rights sensitivity of nursing students, and the systematic education strategy and education program should be developed and applied.

대학 학생선수의 운동스트레스, 자기관리 및 중도포기의 관계모형 검증

서연희(Seo, Yeon-Hee),정구인(Jung, Koo-In) 한국체육과학회 2015 한국체육과학회지 Vol.24 No.3

The purpose of this study is to verify a causal relation between exercise stress of university student athletes, their self-management and their giving-up in the middle. For data processing this study analyzed a total of 304 questionnaire copies with SPSS WIN 18.0 by conducting confirmatory factor analysis, reliability analysis, frequency analysis, descriptive statistics, and correlation analysis. To examine the structural relation of the study model, this study employed LISREL 8.80 to conduct confirmatory factor analysis and structural equation modeling (SEM). The analysis results are presented as follows: First, exercise stress of university student athletes significantly influenced their giving-up in the middle (excessive training, anxiety over the future, economic burden, loss of self-esteem, negative friendship, loss of interest, and unfair hierarchical structure). Secondly, self-management of university student athletes (body management, interpersonal relationship management, training management, and mentality management) significantly influenced their giving-up in the middle (excessive training, anxiety over the future, economic burden, loss of self-esteem, negative friendship, loss of interest, and unfair hierarchical structure).

운동선수의 은퇴기대, 심리적 위기감, 은퇴준비수준 및 재사회화 간의 구조관계

서연희(Seo, Yeon-Hee) 한국체육과학회 2012 한국체육과학회지 Vol.21 No.4

The purpose of this research was to examine the structural relationship among athletes’ retirement expectation, psychological sense of crisis, level of retirement preparation and re-socialization, and to provide them with a practical method for their organized preparation for retirement and the efficient adaptation of themselves to a new environment after retirement. For the research, the survey targeted the elite athletes in the Chonbuk province. For the account of research target’s characteristics and questionnaires, frequency analysis, reliability analysis and correlation analysis were used with SPSS WIN 18.0. In order to verify the structural relationship of the study modeling, confirmative factor analysis and structural equation modeling were operated with LISREL 8.80. The result of this study was as follows: First, athlete’s retirement expectation was statistically significant to a psychological sense of crisis. Second, the retirement expectation made a statistically meaningful influence on the level of retirement preparation. Third, the retirement expectation was influential to re-socialization with statistical significance. Fourth, his/her psychological sense of crisis was not statistically meaningful to re-socialization. Last, his/her level of retirement preparation statistically had a substantial impact on re-socialization.