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김수빈(Subin Kim),비빈프라사드(Bibin Prasad),김중경(Jung Kyung Kim) 한국가시화정보학회 2016 한국가시화정보학회지 Vol.14 No.3
Separation of particles based on different sizes, detection of pathogenic bacteria and isolation of leukocytes from whole blood are typical applications of spiral or helical microchannels. The present study focuses on developing a CD4+ T-cell counting device for monitoring HIV/AIDS patients with the aid of a helical minichannel used for a sample cartridge. For the experiment, 10 μm sized microbeads were used for visualization with a fluorescence imaging system. Alignment of microbeads was investigated in a stationary and spinning sample cartridge filled with glycerol-water mixtures of different densities. The helical minichannel was spun using a DC motor controlled by an Arduino board with a Bluetooth shield. It was found that when the sample cartridge was made stationary, no bead alignment was achieved for a medium with density (0% and 20% glycerol) lower than that of the beads, but when it was spun at 2000-3000 rpm for 1-4 min, an alignment was obtained at the top of the channel facilitating optical detection and enumeration of those microbeads. Since an alignment of microbeads was achieved for a medium with density as that of blood plasma, the same approach can be applied for aligning and counting CD4+ T-lymphocytes in whole blood samples collected from patients.
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김수빈(Subin Kim),비빈프라사드(Bibin Prasad),김중경(Jung Kyung Kim) 한국가시화정보학회 2015 한국가시화정보학회 학술발표대회 논문집 Vol.2015 No.12
Separation of particles based on different sizes, detection of pathogenic bacteria and isolation of leukocytes from whole blood are typical applications of spiral or helical microchannels. Several microfluidic point-of-care (POC) devices have been developed for monitoring HIV/AIDS by counting CD4 cells but researches are still pursuing to develop a more efficient low-cost device that can be used in developing and underdeveloped countries. The present study focuses on developing a CD4+ T-cell counting device for HIV/AIDS monitoring with the aid of a helical microchannel. For the experiment 10 μm sized particles were used for visualization with a fluorescence microscope system. A sample with the viscosity as that of blood and other samples with different viscosities were also prepared to determine the effect of density and viscosity on alignment of the particles. The channels were spun using a DC motor controlled by an Arduino board with a Bluetooth shield. It was found that for low viscosity samples the particles were not aligned when the channel was kept stationary and an alignment was achieved when the channel was spun due to the combination of centrifugal and gravitational forces. For a sample with viscosity as that of blood, alignment of particles were obtained even without spinning. Since an alignment of particles was achieved for a sample with viscosity as that of blood, the same approach can be applied for aligning and counting CD4+ T cells in blood samples collected from patients.