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Violacein을 생산하는 Massilia sp. EP15224 균주
윤상홍 ( Sang Hong Yoon ),백희진 ( Hee Jin Baek ),권순우 ( Soon Wu Kwon ),이창묵 ( Chang Muk Lee ),심준수 ( Joon Soo Sim ),한범수 ( Bum Soo Hahn ),구본성 ( Bon Sung Koo ) 한국미생물생명공학회(구 한국산업미생물학회) 2014 한국미생물·생명공학회지 Vol.42 No.4
비올라세인은 항균, 항암, 항산화, 항말라리아, 항설사 활성과 같은 다양한 생리활성을 가지는 보라색소 물질로 알려져 있다. 본 연구에서는 한국의 산림토양에서 분리되었으며 보라색 색소를 생산하는 EP15224 균주를 선발하여 16S rRNA유전자의 염기서열을 분석하였다. 그 결과 Massilia sp. BS-1과 97%의 가장 높은상동성을 보였다. 또한 16S rRNA 유전자에 근거한 계통분류학적 분석에서 EP15224는 기존 보고된 비올라세인 생산 세균들과는 별개로 구분되는 Massilia속의 새로운 종임을 확인하였다. 이 균주가 생산하는 보라색소물질을 분리하여 LC/MS/MS로 분석한 결과, 분자량이 343.34인 비올라세인과 일치하였다. 또한 비올라세인의 생산효율을 높여주는 최적의 배지성분[glucose 2 g/l, (NH4)2SO4 1 g/l, Na2HPO4· 7H2O 2 g/l, KH2PO4 1 g/l, MgSO4· 7H2O0.1 g/l, L-tryptophan 0.24 g/l]과 배양조건[25oC에서 72시간배양, 250 rpm, 10% 접종량]을 조사하였으며 이 조건에서 액체배양 하였을 때 리터 당 280 mg의 비올라세인이 생산되었다. Violacein has received much attention due to its various important biological activities, including broad-spectrum antibacterial and antifungal activity, anti-malarial, anti-tumoral, anti-oxidant, and anti-diarrheal activities. EP15224 strain isolated from forest soils in Korea was found to be a new species belonged to the genus Massilia based on its 16S ribosomal DNA sequences. The 16S ribosomal DNA of strain EP15224 displayed 97% homology with Massilia sp. BS-1, the nearest violacein-producing bacterium. Strain EP15224 produced bluish-purple pigment well in a synthetic MM2 medium containing glucose, (NH4)2SO4, Na2HPO4·7H2O, KH2PO4, MgSO4·7H2O, and 1 mM L-tryptophan. The chemical analysis of the pigment by LC/MS/MS showed that it is violacein with molecular weight of 343.34. This is the second report on the production of violacein by a Massilia species. In this study, the optimal culture conditions for violacein production were established under which 280 mg/l crude violacein was produced : glucose 2 g/l, (NH4)2SO4 1 g/l, Na2HPO4·7H2O 2 g/l, KH2PO4 1 g/l, MgSO4·7H2O 0.1 g/l, L-tryptophan 0.24 g/l, 25 ml medium in a 250 ml flask, with an inoculumn size of 10% (v/v), 72 h of cultivation with 250 rpm at 25oC.
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구교성 ( Kyo Soung Koo ),김유진 ( Eugene Kim ),백희진 ( Hee Jin Baek ),서희정 ( Hui Jeong Seo ),황영혜 ( Young Hae Hwang ),장이권 ( Yikweon Jang ) 한국환경생태학회 2021 한국환경생태학회 학술대회지 Vol.2021 No.2
In the late 1990s, American bullfrogs and red-eared sliders, imported for food, pets, and religious events in South Korea. By natural or artificial factors, however the non-native species were introduced into nature. The Korean government designated the two species as ecosystem disruptors, and subsequently banned imports and transactions because of the risk to the nature. Unfortunately, with the recent expansion of the pet market, more diverse non-native reptiles are being imported into the country. This increase in imports of non-native organisms is linked to an increase in potential inflows to ecosystem and confounding threats. A recent survey found that nearly 20 species of non-native amphibian-reptiles discovered in the wild. Furthermore non-native amphibian-reptiles are actively bred by people for hobby or profit reason, but it has not been determined how many amphibians and reptiles are artificially bred in South Korea. Therefore in this study, we investigated how various non-native amphibian-reptiles are subject to artificial reproduction. For the collection of data, all non-native amphibian-reptiles were collected from 1st to 31st August 2021. We used searching engine such as Google, YouTube, Naver, and Daum to find breeding recorded. Keywords that can be related to artificial breeding such as "cultivation, breeding, non-native species, and rare species were used to gain accurate data. In the collected data, it was divided into 1) breeding period, 2) breeding location, 3) breeding target species, 4) success or failure of breeding, and 5) breeding subjects. We found 368 cases of artificial breeding. The first case was recorded in 2008 with Burmese python (1 case) and have since increased steadily until 2021. The most of breeding recorded in 2021, with 117 (31.7%) cases. Artificial breeding took place at a total of 146 locations, Gyeonggi-do has the most abundant site of 57 locations, followed by Seoul has 30 locations, Chungnam has 15 locations, Incheon has 10 locations and Gyeongbuk has 8 locations. The breeding population (n=368) had the largest number of 126 lizard cases (34.2%) with 94 snakes, 92 turtles, 54 frogs and 2 salamander cases. There was a total of 129 non-native amphibian-reptiles that were subject to reproduction. Among them, Leopard Gecko was 42 cases followed by corn snake 33, king snake 17, and ball python 17. From 350 cases that identified the breeding results, 75.4% (264) were successful (hatching). 226 cases of breeding were conducted by individuals this is twice as many as 113 cases by companies. The breeding of non-native amphibian-reptiles tended to be concentrated mainly in Seoul and Gyeonggi-do, the metropolitan area. A total of 677 species of non-native amphibian-reptiles were announced in 2019, 19.1% of which were subject to artificial breeding. Among the various non-native amphibian-reptiles, Leopard gecko had the highest frequency of reproduction, which can be related to various factors such as low breeding difficulty, high selling price, and high reproduction rate. A risk assessment of the top 10 species including Leopard gecko will need to be made for preparing the potential introduction into nature. Considering the more cases of individual breeding than businesses and the success rate of breeding exceeds 70%, there is a possibility that the number of non-native amphibian-reptiles will increase rapidly in the future. Therefore management and safeguards for artificial reproduction by individuals and companies must be prepared.
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김정인(Jung-In Kim),한도원(Do-Won Han),윤정아(Jung-A Yun),백희진(Hee-Jin Baek),임상욱(Sang-Wook Lim) 한국생물공학회 2018 KSBB Journal Vol.33 No.2
The purpose of this study was to investigate pancreatic lipase inhibitory activities and antioxidant activities of carrot juice and fermented carrot vinegar in vitro. Carrot juice was prepared using a low-speed masticating juicer. Carrot juice was fortified with pear extract to 24 obrix, and then alcoholic fermentation was conducted using Saccharomyces cerevisiae (EC-1118) (3%) at 30℃. The alcohol fermentation product was fermented using Acetobacter pasteurianus (KCCM 12654) (5%) at 25℃ to produce carrot vinegar. Pancreatic lipase inhibitory activity and DPPH radical scavenging activity of carrot vinegar were 4.1 and 4.6 times higher than that of carrot juice, respectively (p<0.01). Total polyphenol contents of carrot juice and vinegar were 8.6 and 18.1 mg TAE/100 mL, respectively. Flavonoid contents of carrot juice and vinegar were 1.0 and 10.5 mg CTE/100 mL, respectively. The result demonstrated that fermented carrot vinegar could be a possible candidate for anti-obesity agent.
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