우리나라 콩 육종의 현황과 연구발전 계획

백인열 ( In Youl Baek ),박금룡 ( Keum Yong Park ) 한국콩연구회 2006 韓國콩硏究會誌 Vol.23 No.1

연구논문 : 자연과학 ; 남부지역에서 비닐피복에 따른 콩 생육특성 및 수량성 차이

백인열 ( In Youl Baek ) 한국국제농업개발학회 2012 韓國國際農業開發學會誌 Vol.24 No.1

Nowadays P. E. film mulching for weed control and moisture preservation is popular in soybean cultivation of southern region of Korea. Some merits of P. E. film mulching are moisture preservation, soil aggregation, weed control and growth promotion due to increase soil temperature. The rearrangement of seeding date of soybean is necessary to prevent overgrowth of soybean and promote healthy growth. As global warming increases average world temperature, the demanding of re-establishing soybean cultivation system, such as seeding date, has been increased. Therefore, we discussed seeding date accompanying maturity types as soybean was cultivated on condition of P. E. film mulching. Four varieties, Saeol, Seonyu, Daewon and Pungsannamul were seeded on May 10, May 25, June 10 and June 25 in P. E. film mulching and non-mulching field. It is required more mean days of flowering in nonmulching and more days of maturity in P. E. film mulching. In R5 stage completed organ structure dry matter weight was heavier and LAI was ranged 5 to 7 which was optimum LAI. Number of pods and number of seeds per plant in P. E. film mulching were higher on June 25 seeding and 100-seed weight was heavier through all seeding date of P. E. film mulching. Seed yield in P. E. film mulching cultivation was higher on May 25 and June 10 seeding. Occurrence of damaged seeds showed no difference between two cultivation types and among seeding dates. Major diseases and insect pests were bacterial pustule, black root rot, wild fire and stink bugs. And that, occurrence of root rot and stink bugs influenced to yield decrease. Protein content of soybean seed in P. E. film mulching was lower in all seeding dates. Fatty acid compositions showed no difference between two culivation types and among seeding dates. But C18:1 composition was higher on May 10 and May 25 seeding in P. E. film mulching cutivation and C18:3 composition was similar between two cultivation types. Isoflavone content was higher in P. E. film mulching cultivation and higher as seeding date was delayed. Summerizing above results optimum seeding date for P. E. film mulching cultivation ranged from May 25 to June 10 in southern region of Korea.

장류용 내병 내도복 다수성 콩 신품종 ‘대하 1호’

백인열(In-Youl Baek),김현태(Hyun-Tae Kim),고종민(Jong-Min Ko),한원영(Won-Young Han),박금룡(Keum-Yong Park),오기원(Ki-Won Oh),하태정(Tae-Joung Ha_,신상욱(Sang-Ouk Shin),윤홍태(Hong-Tae Yun),문중경(Jung-Kyung Moon),오영진(Young-Jin Oh) 한국육종학회 2013 한국육종학회지 Vol.45 No.1

A new soybean cultivar for soy-paste, ‘Daeha 1’, was developed by soybean breeding team in the Yeongnam Agricultural Research Institute (YARI) in 2008. A promising line, SS97214-S-S-S-15, was selected from the combination between ‘Suwon192’ and a pedigree came from cross combination between ‘Jangyeobkong’ and ‘Hwaeomputkong’. It was designated as the name of ‘Milyang 164’. It had good result from regional adaptation yield trial (RYT) for three years from 2006 to 2008 and released as the name of ‘Daeha 1’. It has a determinate growth habit, white flower, grey pubescence, yellow seed coat, yellow hilum, large spherical seed (25.4 grams per 100 seeds). ‘Daeha 1’ is tolerant to soybean mosaic virus and bacterial pustule, the major soybean disease in Korea. The average yield of ‘Daeha 1’ was 2.62 ton per hectare in the regional yield trial (RYT) carried out for three years from 2006 to 2008, which was 5 percent higher than that of check cultivar, ‘Taekwangkong’.

大豆屬(Glycine spp.)의 形態的 特性에 의한 類緣關係 分析

In Youl Baek(白寅烈),Doo Chull Shin(申斗澈),Hyun Tae Kim(金賢泰),Hyung Soo Suh(徐亨洙),Yong Ho Kwack(郭龍鎬),Yun Jin Oh(吳潤鎭) 한국육종학회 1997 한국육종학회지 Vol.29 No.2

Discovery and transfer of useful characters of wild species into the elite lines may facilitate the development of crop varieties with broad genetic bases. In this study, morphological relationships among fourteen Glycine species was analyzed on basis of twenty-five morphological characters. The upper five principle components contracted from twenty-five morphological characters by the principle component analysis accounted for about 72% of the total variation. The subgenus Soja; G. max, G. gracilis, and G. soja were clearly seperated from the subgenus Glycine; G. arenaria, G. falcata, G. canescens, G. latifolia, G. microphylla, and G. tomentella by the scatter diagrams distributed on the plane of the first and second or the first and third principle components. The cluster analysis and morphological characteristics of G. cyrtoloba and G. curvata were very similar, but they are distinguished from tri-compound leaves at the unifoliate leaf node. Fourteen Glycine species could be classified into eight major groups at the average distance of 0.75 and fourteen sub-groups at the average distance of 0.45, that is, into Group Ⅰ: G. max; Group Ⅱ: A: G. gracilis, B: G. soja; Group Ⅲ: G. microphylla; Group Ⅳ: G. tomentella (2n=38, 40, 78, 80) ; Group Ⅴ: A: G. tabacina (2n=120), B: G. tabacina(2n =40, 80) ; Group Ⅵ: A: G. clandestine, B: G. canescens; Group Ⅶ: A: G. latifolia, B: G. tabacina (2n=40), G. latrobeana, C: G. cyrtoloba, G. curvata; and Group Ⅷ: A: G. falcata, B: G. arenaria.

RAPD方法에 의한 콩屬의 種間 類緣關係 分析

In Youl Baek(白寅烈),Yong Hwi Yoon(尹用輝),Dao Chull Shin(申斗澈),Gyu Hwan Park(朴圭煥),Young Hun Hwang(黃永鉉),Dal Ung Kim(金達雄) 한국육종학회 1997 한국육종학회지 Vol.29 No.3

Effective conservation and the use of plant genetic resources are essential for future agricultural progress. In this study, the genetic relationships among fourteen species of Glycine were analyzed based on the RAPDs. A total of fourteen species (twenty one accessions) in the genus Glycine were identified by the frequency of RAPDs polymorphisms. Ten primers out of thirty primers tested, were selected for the reliability and repeatability of banding patterns. The amplification of the genomic DNA produced eighty two polymorphic band patterns with the average of 8.2 bands per primer. By Nei and Li’s formula, the mean 1-F value (genetic similarity) was 0.461, and the highest value was 0.774 (G. max : G. microphylla) and the smallest one was 0.128 (intraspecies G. max). That of interspecies in the subgenus Glycine was 0.620 and 0.250 in the highest and the lowest cases, respectively. Thus the genetic similarities were much variable within the interspecies of subgenus Glycine but those were somewhat small between the subgenus Glycine and Soja. By UPGMA (unweighted pair group method using an arithmetic average) cluster analysis based on 1-F value, the fourteen species of Glycine could be classified into four major-groups (genetic similarity (GS) : 0.45) and nine sub-groups (GS : 0.35), that is, into Group Ⅰ : subgenus Soja : G. max, G. sofa, G. gracilis; Group Ⅱ : G. falcata ; Group Ⅲ : A : G. latrobeana, G. canescens, G. microphylla, B : G. curvata, G. cyrtoloba, C : G. clandestina, D : G. tabacina(2n=80) ; Group Ⅳ : A : G. arenaria, B : G. tomentella (2n=38, 40, 78, 80), C : G. tabacina (2n=40, 120), G. latifolia. By multidimensional scaling (MDS), the subgenus Glycine and Soja were clearly classified in the clustering of genetic relationship distance. The genetic relationship distance was closely identified between G. max of the cultigen and G. clandestina, G. tomentella, G. cyrtoloba, G. curvata of the subgenus Glycine.

국내 콩 유전자원의 Isoflavone 함량 변이

정명근,강성택,한원영,백인열,김현경,신두철,강남숙,황영선,안영남,임정대,김관수,박시형,김선림,Choung, Myoung-Gun,Kang, Sung-Taeg,Han, Won-Young,Baek, In-Youl,Kim, Hyeun-Kyeung,Shin, Doo-Chull,Kang, Nam-Suk,Hwang, Young-Sun,An, Young-Nam,Lim, 한국작물학회 2006 Korean journal of crop science Vol.51 No.suppl1

고 isoflavone 함유 콩 유전자원 선발 및 품종육성의 기초자료로 활용하기 위해 국내 콩 주요품종 43계통 및 보존 유전자원 648계통의 isoflavone 함량을 분석한 결과를 요약하면 다음과 같다. 1. 한국산 콩 주요 43품종의 평균 isoflavone 함량은 daidzein이 $484.4{\pm}223.9{\mu}g/g$, genisteinol $448.0{\pm}156.2{\mu}g/g$, glyciteinol $84.9{\pm}48.7{\mu}g/g$, total isoflavoneol $1,017.3{\pm}390.0{\mu}g/g$을 나타내었다. 2. 공시된 주요 품종의 isoflavone 함량은 석양풋콩이 $2,002{\mu}g/g$으로 가장 높은 함량을 나타내었고, 황금콩이 $277{\mu}g/g$으로 가장 낮은 함량을 나타내었다. 또한 콩 주요 43품종을 용도별로 분류하여 isoflavone 함량을 비교한 결과 풋콩 및 올콩이 $1,438{\pm}493.7{\mu}g/g$을 나타내어 조사된 용도별 콩 분류 중 가장 높은 양상을 나타내었다. 3. 국내 보존 콩 유전자원 648계통을 대상으로 isoflavone 함량을 조사한 결과 IT 180001계통이 $3,479{\mu}g/g$의 함량을 나타내어 가장 높은 양상을 나타내었고, IT 910878 계통은 $82.61{\mu}g/g$의 total isoflavone을 함유하여 가장 낮은 계통으로 조사되어 보존 유전자원간에 큰 함량변이를 나타내었다. Isoflavone in soybean is known to have the important physiological functions such as antioxidative, estrogenic, and anti-tumor activities. Therefore, isoflavone content is considered as important aspect of soybean quality. The objectives of this study are to determine the isoflavone contents of soybean germplasms including major cultivars in Korea, and to provide the basic information of isoflavone far development soybean cultivar containing high isoflavone content. Among 43 Korean major cultivated soybean samples, the total isoflavone contents were varied from $277.0{\mu}g/g\;to\;2,002.0{\mu}g/g$, and the average total isoflavone content was $1,017.3{\pm}390.0{\mu}g/g$. Total isoflavone content of Seokryangputkong $(2,002{\mu}g/g)$ was the highest among those of the other cultivated soybeans. On the other side, Hwangkeumkong $(277{\mu}g/g)$ contained lower isoflavone than other cultivars. The total isoflavone content of vegetable use or early maturity soybean cultivar $(1,438{\pm}493.7{\mu}g/g)$ showed more higher total isoflavone content than others. The daidzein, glycitein, genistein and total isoflavone contents of 648 soybean germplasms collected in Korea, were ranged $16.9{\sim}2,019.8{\mu}g/g,\;0{\sim}294.9{\mu}g/g,\;38.5\sim1,337.7{\mu}g/g,\;and\;82.6{\sim}3,479.2{\mu}g/g$, respectively, and total isoflavone content of IT 180001 line $(3,479.2{\mu}g/g)$ was the highest among those of other soybean germplasm lines.

콩 이모작 적응 조숙 다수성 품종 ‘황금올’

고종민(Jong Min Ko),백인열(In Youl Baek),한원영(Won Young Han),이병원(Byong Won Lee),이영훈(Young Hoon Lee),신상욱(Sang Uk Shin),강범규(Beom Kyu Kang),김현태(Hyun Tae Kim),하태정(Tae Joung Ha),문중경(Jung Kyung Moon),이석기(Seuk Ki L 한국육종학회 2016 한국육종학회지 Vol.48 No.3

A soybean cultivar for soy-paste, ‘Hwangkeumol’, was developed from the cross between SS92414 (crossing line of ‘Pokwangkong’ and ‘Suwon163’) and ‘Hwaeomputkong’ by soybean breeding team in the National Institute of Crop Science (NICS) in 2010. A promising line, SS99409-2B-11-5-4, was selected and designated as the name of ‘Milyang202’. It was prominent and had good result from regional adaptation yield trials for three years from 2008 to 2010 and released as the name of ‘Hwangkeumol’. It has a determinate growth habit, white flower, gray pubescence, yellow seed coat, yellow hilum, spherical seed shape and large seed (28.6 grams per 100 seeds). Maturity date of ‘Hwangkeumol’ was as early as September 15th. Therefore it is suitable for double cropping system. ‘Hwangkeumol’ was resistant to bacterial pustule and resistant to soybean mosaic virus, the major soybean diseases in Korea and tolerant to lodging in fields. The average yield of ‘Hwangkeumol’ was 2.51 ton per hectare in the regional yield trials carried out for three years from 2008 to 2010.

Simple Sequence Repeat Markers Linked to Quantitative Trait Loci Controlling Seed Weight, Protein and Oil Contents in Soybean

김현경,강성택,정명근,정찬식,오기원,백인열,손병구,Kim, Hyeun-Kyeung,Kang, Sung-Taeg,Choung, Myoung-Gun,Jung, Chan-Sik,Oh, Ki-Won,Baek, In-Youl,Son, Beung-Gu Korean Society of Life Science 2006 생명과학회지 Vol.16 No.6

콩은 전 세계 시장의 48%를 차지하는 중요한 유료작물이다. 콩 종자를 구성하는 양적인 부분과 질적인 부분의 개선은 콩 육종 목표의 중요한 부분이다. 단백질함량과 종실의 크기는 두부와 콩나물의 질을 평가하는 중요한 특성이다. 따라서 본 연구는 콩에서 종실의 크기와 단백질 및 oil 함량을 조절하는 양적형질유전자좌(QTLs)를 확인하기 위하여 실시하였다. 시험재료로는 큰올콩과 신팔달콩을 교배한 후 $F_2$유래 $F_10$세대의 RIL을 이용하였으며, 이를 바탕으로 종실의 무게와 oil 및 단백질 함량과 관련된 QTLs를 탐색하였다. 종실의 무게와 단백질 및 oil 함량의 협의의 유전력은 각각 0.8과 0.78 및 0.71을 나타내었다. 종실의 무게와 관련된 QTLs는 연관군 F, I와 K의 세 개의 독립적인 QTLs를 확인하였다. 단백질함량과 관련된 QTLs는 연관군 D1b, E, H, I와 L의 다섯 개의 독립적인 QTLs를 확인하였다. 그리고 oil 함량과 관련된 QTLs는 연관군 D1b, E, G, I, J와 N의 여섯 개의 독립적인 QTLs를 확인하였다. oil 및 단백질 함량과 관련된 QTLs는 연관군 D1b, E와 I에서 공통적으로 나타났다. 따라서 이들 주요 QTL은 콩 품종 육성과정에서 품질의 개선하기 위한 선발 마커로서 활용가치가 높은 것으로 판단된다. Soybean [Glycine max (L.) Merr.] is an important crop, accounting for 48% of the world market in oil crops. Improvement of the quality and quantity of soybean seed constituents is one of the most important objectives in soybean breeding. Protein content and seed size are important properties to determine the quality of tofu and soy sprouts respectively. The objective of this study was to identify quantitative trait loci (QTLs) that control seed weight, protein and oil content in soybean. The 117 $F_{2:10}$ recombinant inbred lines (RlL) developed from a cross of 'Keunolkong' and 'Shinpaldalkong' were used. Narrow-sense heritability estimates based on a plot mean on seed weight, protein and oil content were 0.8, 0.78 and 0.71, respectively. Four independent QTLs for seed weight were identified from linkage group (LG) F, I and K. Five QTL for protein content were located on LG D1b, E, H, I and L. Oil content was related with six QTLs located on LG D1b, E, G, I, J and N. Protein and oil content have three common QTLs on LG D1b, E and I. Thus, we identified major loci improving soybean seed quality.

다수성 내탈립 녹자엽 검정콩 ‘청자5호’

서정현(Jeong Hyun Seo),강범규(Beom Kyu Kang),백인열(In Youl Baek),한원영(Won Young Han),최만수(Man Soo Choi),이병원(Byong Won Lee),윤홍태(Hong Tai Yun),김홍식(Hong Sik Kim),고지연(Jee Yeon Ko),김준회(Jun Hoi Kim),허수빈(Su Vin Heo),황 한국육종학회 2022 한국육종학회지 Vol.54 No.4

‘Cheongja5’ is a black seed coat soybean cultivar developed from crossing ‘Milyang181’ and ‘YS1886 (Cheongdu1/Tanbaguro)’ in 2007. Promising lines were selected using the pedigree method from F3 to F5. The preliminary and advanced yield trials were conducted in 2013 and 2014, respectively. Regional yield trials (RYT) were conducted in seven regions from 2015 to 2017. ‘Cheongja5’ has a determinate growth habit, oval leaflet shape, brown pubescence, and white flowers. The seed of ‘Cheongja5’ has a black seed coat color with green cotyledon. Flowering and maturing dates were Aug. 3 and Oct. 24, respectively. Regarding the quantitative characteristics, ‘Cheongja5’ has large seed size (37.0 g/100-seed weight) and more pods than ‘Cheongja3’. ‘Cheongja5’ was tolerant to pod shattering in field and indoor RYT tests. ‘Cheongja5’ was resistant to bacterial pustule in the field and soybean mosaic virus (strains G6H and G7H) in the inoculation test. Although its seed quality-related characteristics were almost similar to those of ‘Cheongja3’, it contained more anthocyanin which is a main functional component of black soybean. The mean yield of ‘Cheongja5’ in the RYTs was 343 kg/10 a which was 30% higher than that of ‘Cheongja3’. ‘Cheongja5’ is expected to be widely cultivated as a material for making soy-food that uses black soybean because of its large seed size and high yield potential (Registration No. 7687).

국내 콩(Glycine max) 품종 형질전환 초기조건 확립

이기정,서진경,이혜영,전은희,신상현,이재헌,김도훈,고종민,한원영,백인열,오병준,정영수,Lee Ki-Jung,Seo Jen-Kyung,Lee Hye-Young,Jeon Eun-Hee,Shin Sang-Hyun,Lee Jai-Heon,Kim Doh-Hoon,Ko Jong-Min,Hahn Won Young,Baek In-Youl,Oh Boung-Jun,Chung 한국생명과학회 2006 생명과학회지 Vol.16 No.2

경제적으로 중요한 식용작물인 콩(Glycine max)의 초기형 질전환 효율증대를 위하여 연구를 수행하였다. 높은 초기형 질전환 조건 확립을 위하여 기초실험으로 Agrobacterium 감 염에 순응적인 국내 콩 품종을 스크린 하였으며, 적정한 agar 농도와 선발항생제 hygromycin 농도 규명, 상처방법, explant의 치상방향, 호르몬 전처리, 액체배지 선발 등 형질 전환 초기효율에 미치는 영향을 transient GUS 분석을 통하여 알아보았다. Agrobacterium 감염에 순응적인 품종을 선발하기 위하여 32개의 국내 콩 장려품종을 스크린한 결과, 일품검정콩, 은하콩, 만리콩, 대원콩 등 14개의 순응형 품종을 선발하였으며, 효율적인 제균제로는 cefotaxime이, 효율적인 선발항생제로서는 hygromycin이 선택되었다. Hygromycin 농도는 10-15 ppm이 효율적이었고, agar 농도 0.6-0.8%, explant의 치상방향은 향배축(adaxial side)을 down하여 치상하였을 때가 높은 GUS ($\beta$-glucuronidase)발현빈도를 나타냈고 상처방법은 scalpel보다. 동양침을 사용하였을 때 높게 나타났다. 형질전환 체계확립을 위하여 호르몬 전처리를 한 결과, BA 5 ppm 과 10 ppm을 처리하였을 때 후기 선발에서 높은 생존율을 보였고, 전처리 후에 액체배지에서 선발을 하였을 때 비형질 전환체(escape)의 빈도를 크게 낮출 수 있었다. 또한 여러 조건의 결과를 비교하여 볼 때, 국내 콩 품종 중에서 형질전환을 위한 재료로써는 은하콩이 가장 적합한 것으로 생각된다. In order to establish highly efficient gene transfer condition at early stage of soybean transformation, various experiments were performed and compared their efficiencies by transient GUS analysis; those conditions are genotype determination of Korean soybean cultivars for amenability to Agro-infection, appropriate agar and selective agent concentration, orientation of explant placement, hormone pre-culture, and liquid selection condition. In the genotype screen of Korean soybean varieties, 14 amenable genotypes were selected. For efficient Agrobacterium washing, cefotaxime was chosen and hygromycin at the concentration of 10 and 15 ppm was used as selection agent in the media. Agar concentration was slightly better in 0.6% and 0.8% for both shoot and callus formation, and explant placement with adaxial side down showed high frequency of GUS expression. For wounding treatment, oriental needle was efficient than scalpel for shoot formation and gene transfer. To increase the frequency of gene transfer, hormone pre-treatment was applied. BA at the concentration of 5 and 10 ppm resulted in better survival at the late stage of selection in shoot elongation media. Selection in liquid media after hormone pre-treatment seemed to be effective to remove the escaped non-transformants at early stage of procedure. Considering the results obtained, Eunhakong could be the first choice as a material for soybean transformation among Korean soybean genotypes.