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청피홍심무우 ( Raphanus sativus cv. Chungpihongsim ) 의 모상근 배양에 의한 안토시아닌 생성
안준철(Jun Cheul Ahn),백윤웅(Yun Woung Paek),강영희(Young Hee Kang),황백(Baik Hwang) 한국식물학회 1992 Journal of Plant Biology Vol.35 No.1
The hairy root culture of Raphanus sativus cv. Chungpihongsim was established by transformation with Agrobacterium rhizogenes A_4. The transformed roots grew well in adjusted Murashige and Skoog medium to 1/2 basal salts, pH5.2,3% sucrose. Agropine and mannopine, poine synthesized in the transformed tissue were detected in the extract of hairy roots. When 2,4-D and kinetin were added in culture medium of hairy roots, the synthesis of anthocyanin was induced with disorganization of hairy root. Especially, addition of 0.45 μM 2,4-D and 2.3 μM kinetin showed the maximum synthesis of anthocyanin. Pattern of anthocyanin synthesized in transformed roots was somewhat different from that of ordinary roots. However, aglycone part of all anthocyanin was identified as pelargonidin. The content of total anthocyanin in this sample was tentatively calculated 0.49 mg/g fresh weight.
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Raphanus sativus Var. Chungpihongsim의 hairy root 배양에 의한 anthocyanin 생성
황성진,백윤웅,고경민,안준철,황백 全南大學校 農漁村開發硏究所 1991 農業科學技術硏究 Vol.26 No.-
청피홍심무우를 재료로 A. rhizogenes A₄균주를 접종하여 hairy root를 유도하였으며 유도된 hairy root는 paper electrophoresis를 통하여 mannopine과 agropine이 검출되어 형질전환 되었음을 확인하였다. Hairy root는 MS의 기본염을 1/2로 희석시킨 1/2 MS, sucrose 3%, pH 5.2로 조성된 배지에서 최적인 성장을 보였다. 유도된 hairy root는 각 strain사이에 색소능에 다소간의 차이를 보였으며 2, 4-D kinetin의 적절한 조합의 처리구에서는 탈분화와 더불어 색소가 형성되었고, 그 중 2, 4-D와 kinetin을 각각 1 ppm, 5 ppm 처리하였을 때 최대 색소능을 보였다. hairy root의 탈분화시 형성되는 색소는 원 식물 뿌리의 주 색소인 pelargonidin 계열의 색소로 추정되며 총 색소량은 49mg/100g(F.W.)로 잠정 계산되었다. Hairy root induced following inoculation of the root disc and plantlet fragment of R. sativus Var Chungpihongsim with A. rhizogenes A₄. The hairy root contained agropine and mannopine. Medium contained 1/2 MS salt, sucrose 3%, pH 5.2 was optimized to growth of hairy root. Depending on the hairy root line, the level of pigment in hairy root varied somewhat . When 2, 4-D and kinetin were added in hairy root, the synthesis of pigment induced with deorganization of hairy root. Especially, addition of 2, 4-D 1 ppm and kinetin 5 ppm showed the maximum synthesis of pigment. Compared with anthocyanin extract of mature root three pigments were assumed to acylated pelargonin. The content of anthocyanin in these sample was 49mg/100(F.W.).
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