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김홍집,배종찬,황규인,공운영 한국농화학회 1979 Applied Biological Chemistry (Appl Biol Chem) Vol.22 No.4
RNA degrading bacteria were isolated from soil of Korea. One strain (no. JSC-114), having strong 5'-phosphodiesterase activity, was identified as belonging to the genus Streptomyces on the basis of taxonomic characteristics. The optimum conditions of 5'-phophosdiesterase production were found at 30℃ for 4 day in a medium containing 4.5% of soluble starch, 0.15% of peptone, 0.6% of yeast extract, 0.1% of MgSO₄·7H₂O, 0.01% of CaCl₂·2H₂O, 0.25% of KNO₃, and 0.5% of KH₂PO₄(pH 7.0). The maximum production rate of 5'-nucleotides from yeast RVA was 95% at 40-45℃ for 4hrs, and the products were identified as 5'-IMP, 5'-GMP, 5'-CMP and 5'-UMP(5.5 : 5.0 : 4.9 : 5.0).