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인삼 조직배양에 있어 CO₂ 처리가 식물체 분화 및 생육에 미치는 영향
정찬문(Chan Moon Chung),배길관(Kil Kwan Bae),Masatoshi Aoki 한국약용작물학회 2000 한국약용작물학회지 Vol.8 No.1
This experiment was conducted to investigate the effects of length of storage period under low temperature, CO₂ enrichment and addition of plant growth regulators in Murashige and Skoog medium on the plant regeneration of Korean ginseng (Panax ginseng C.A. Meyer). Seeds were treated for 60 and 80 days respectively under 5℃ environment. 2500ppm of CO₂ was enriched by ventilation. Three plant growth regulators added to the medium were Indolbutyric acid, Benzyladenin and Gibberellic acid (GA3). The result indicated that : The capacity of differentiation was higher in the aged cotyledons from the seeds treated for 80 days under low temperature condition than in those treated for 60 days. CO₂ enrichment had stimulating effects on the growth and development of shoot primordium significantly but less effects on the formation of adventitious buds. From one zygotic embryo hundreds of plantlets were differentiated. CO₂ enrichment had effects on the formation of both indirect somatic embryo and direct somatic embryo. Indirect somatic embryo showed little growth and differentiation, being undifferentiated vascular stele and epicotyl. Direct somatic embryos were formed on the epidermis of backside basal part of cotyledon. Those embryos developed to whole plant having latent bud.
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인삼 조직배양에서 CO₂ 처리가 multi-shoot 분화 및 사포닌 함량에 미치는 영향
정찬문(Chan Moon Chung),배길관(Kil Kwan Bae) 한국약용작물학회 1999 한국약용작물학회지 Vol.7 No.4
This experiment was conducted to study the effect of CO₂ (0, 2,500, 5,000, 10,000ppm) enrichment by enabling ventilation on micropropagation of multi-shoot and on the saponin contents in vitro in Korean ginseng(Panax ginseng C. A. Meyer). Embryo was cultured in Murashige and Skoog medium added 3㎎/ℓ of Indolbutyric acid, Benzyladenin and Gibberellic acid (GA₃), respectively. CO₂enrichment had little effects on the number of adventitious buds and shoots originated from adventitious buds. The ratio of differentiated shoots to adventitious buds were about 50% in CO₂enrichment treatment. The shoots originated from adventitious bud showed more rapid growth and had larger leaf area than the shoots originated from the leaf primordia did. The number of shoot primordia was the highest in 2,500ppm of CO₂enrichment treatment. On the contrary, 10,000ppm of CO₂ enrichment made smaller the number of shoot primordia and ratio of shoots to shoot primordia. The range of shoots differentiated was from shoot primordia were 15.4 to 23.9. The rate of dry weight of cultured shoots showed lowest(7.5%) in control and highest(8.59%) in 2,500ppm of CO₂ enrichment. Rate of in vitro flower in control was 7.6% and that in 2500ppm of CO₂ was about twice (15.7-16.3%) as much as in control. Flower number per a embryo cultured was about 1.2-1.3. In the multi-shoots with callus enriched by 2,500ppm of CO₂, the contents of crude saponin and ginsenosides in multi-shoots alone were higher than in multi-shoots with callus. The characteristics of ginsenosides in multi-shoots were especially the higher content of ginsenoside Rd, Re, and Rg₁.
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卞珠燮,趙成鎭,盧載榮,裵吉寬 충북대학교 엽연초연구소 1982 煙草硏究 Vol.9 No.-
This experiment was conducted to investigate the influence of pH, concentration of iron and phosphate and their interaction on the formation of nicotine in tobacco plants. For different levels of pH and phosphate respectively and two levels of iron were treated in thin experiment. The experimented tobacco plants were cultured by water culture in the greenhouse. The results are summarized as follows. 1.Stem height, stem diameter, length and with of the largest levels and number of total leaves increased at the plot of pH 5.5 and pH 7.0. 2.In the plot of treated 0.5 ppm of iron and 30 ppm of phosphate, weight of total leaves and weight of unit leaf area were large. It is considered that the yield increase was due to the increase of the weight of unit leaf area. 3.The root weight increased by treatment of 0.5 ppm of iron. And the root weight of the plot treated 0.5 ppm of iron increased by the treatment of 30 ppm of phosphate. 4.The difference nicotine content in the loaves among the treatments showed the same tendency as the root But there were no differences between the nicotine content at different concentation of phosphate in the case of 1.0 ppm of iron, and there were no differences between the nicotine content at different concentration of iron in the case of 60 ppm of phosphate.
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