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Salmonella othmarschen에 의한 집단식중독사례의 분자역학적 분석
방선재,박광희,박명기,권연옥,고환욱,Bang, Seon-Jae,Park, Kwang-Hee,Park, Ki-Myung,Kwon, Yeon-Ok,Ko, Hoan-Uck 한국환경보건학회 2008 한국환경보건학회지 Vol.34 No.2
The strains of Salmonella othmarschen and Norovirus isolated from outbreak A, the funeral ceremonies incidents in Ouri-shi of Oyeonggido in, 2007, were subjected to genotyping by using PFGE and extraction of RNA by using RT-PCR. 24 Salmonella othmarschen were isolated from 37 cases (patients 12 cases, cookers 4 cases, foods 7cases, knife, kitchen board, dish towel 4 cases, etc) and PFGE patterns showed that 22 strains isolated from the same incident were almost of the same pattern and 2 strains exhibited 50% of the pattern. Antimicrobial susceptibility patterns were such that there were 22 case susceptibility patterns and 2 case resistance patterns (strain 4: AM, CF, CIP, NA, TE TIC and strain 13: CF, CZ). Norovirus G1 (1case), Norovirus G2 (7cases), Norovirus G1,G2 (1case) were detected by using RT-PCR. The fine typing ability and reliability of PFGE and BioNumerics are helpful for establishing out the foodborne pathogens of outbreaks.
다중이용시설내 냉각탑수 및 실내공기에 존재하는 레지오넬라균 조사
방선재(Seon-Jae Bang),박용배(Yong-Bae Park),강정복(Jeong-Bok Kang),김중범(Jung-Beom Kim),김종찬(Jong-Chan Kim) 한국실내환경학회 2006 한국실내환경학회지 Vol.2 No.2
This study was carried out to investigate the presence of L. pneumophila in indoor air and water collected from 692 air conditioning cooling towers at different public facilities. For these 4 years (2001~2004) of investigation, water samples were collected in high air conditioner operating month (from July to September) at department stores, hotels, offices, hospitals, discount stores, and public agencies. It was found that L. pneumophila was present in water samples from 47 air conditioning cooling towers. The detection rate of L. pneumophila was 7.6% in 2001, 10.7% in 2002, and 9.5% in 2003, respectively. When we compared the 4 air conditioner operating months, the highest rate of L. pneumophila detection was obtained in the water samples of July. The detection rate of L. pneumophila differed among different facilities. The highest detection rate of 17.9% was found in samples from department stores. L. pneumophila was detected similarly in water samples from hospitals (8.3%) and offices (8.2%). pH, temperature, and turbidity in the 47 L. pneumophila positive water samples ranged from pH 7 to 9, from 25℃ to 38℃, and from 1.0 to 3.5, respectively.
식품중 발기부전 치료제 및 유사물질 함량에 관한 조사연구
홍해근(Hae-Geun Hong),방선재(Seon-Jae Bang),박광희(Kwang-Hee Park),이인숙(In-Sook Lee),박민정(Min-Jung Park),박정화(Jeung-Hwa Park),김종찬(Jong-Chan Kim) 대한환경위생공학회 2007 대한환경위생공학회지 Vol.22 No.3
To det ermine 8 anti-impotence drug/drug-like compounds such as sildenafil, vardenafil, tadalafil, homosildenafil, hydroxyhomosildenafil, aminotadalafil, pseudovardenafil and hongdenafil in foods, simultaneously, high performance liquid chromatograrhy(HPLC) and liquid chromatograthy-mass spectrometry (LC/MS) were used. The HPLC/UV analysis was performed on a column of capcellpak C₁? with 0,1 % sodium-1-hexansulfonate in 0.2M ammonium formate/acetonitrile as a mobile phase. Mass spectra of the compounds by LC/MS were investigated with SCAN mode(Mass range and Fragment voltage) and SIM (Selected Ion Monitoring) mode (Ion target and Fragment voltage). The results follow as; 1. The HPLC/UV analysis was detected from 5 out of 63 samples. The content of sildenafil was in the range of 32.80 ppm~60,13 ppm from 4 out of 5 samples, The contents of sildenafil, vardenafil, homosildenafil were in the range 47,14 ppm from lout of 5 samples. 2. The conformed result of LC/MS was equal of detected from 5 out of 63 samples in HPLC/UV analysis. An easily available, simultaneous determination of 8 standards in adulterated health related foods was established by using a combination of LC/MS methods.