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Carbamate계 살충제에 의한 Cholinesterase활성의 저해
김정호,박홍재,박병윤 한국환경과학회 2002 한국환경과학회지 Vol.11 No.4
Carbamate 농약에 대한 cholinesterase(ChE)활성 저해 관계를 규명 하고자 in vivo와 in vitro 실험을 하였다. ChE의 최적 pH는 8.0이었다. 병아리 혈장의 ChE 활성은 기질로 acetylcholin를 사용할 경우 24.6 μmol/min/g protein이었다. in vivo에서 BPMC을 0.32 mg/kg 경구 투여한 15분 후에 ChE 활성이 60% 까지 저해되었으며 그후 12시간 후에 97% 까지 회복이 일어났다. in vitro에서 효소 활성이 50%로 저해되는데 필요한 농도인 I_50이 Phenyl N-methylcarbamate계에 XMC는 329 ㎍/l였고, metolcarb은 214 ㎍/l였다. BPMC는 111 ㎍/l, propoxur는 107 ㎍/l, isoprocarb는 104 ㎍/l 이었다. Aromatic N-methylcarbamate계에 속하는 carbaryl의 I_50은 280 ㎍/l이었고, carbofuran 114 ㎍/l이었다. This study was carried out with the inhibition of the cholinesterase activity by carbamate insecticides in the chicken in vivo and in vitro. The optimum pH of cholinesterase was 8.0. The cholinesterase activity used the acetylcholin as substrate in plasma was 24.6 μmol/min/g protein. After oral administration with 0.32 mg/kg of BPMC as carbamate pesticide, the cholinesterase activity was inhibited to 60% of control after 15min in vivo. Then the recovery of cholinesterase activity followed to 97% of control after 12hr. I_50, such as concentration required for 50% inhibition of enzyme activity, of phenyl N-methylcarbamate were 329 ㎍/l of XMC, 214 ㎍/l of metolcarb, 111 ㎍/l of BPMC, 107 ㎍/l of propoxur and 104 ㎍/l of isoprocarb. I_50 of aromatic N-methylcarbamate were 280 ㎍/l of carbaryl and 114 ㎍/l carbofuran.
환경 독성 Peptide의 인지질과의 상호 작용 특성 분석
이봉헌,박홍재 한국환경과학회 2003 한국환경과학회지 Vol.12 No.1
The interaction of mastoparan B, a cationic tetradecapeptide amide from the hornet Vespa basalis, with phosphilipid bilayers was with synthetic mastoparan B and its analogue with instead of hydrophobic 12th amino acid residue in mastoparan B. MP-B and its derivative, [12-Ala]MP-B were synthesized by the solid-phase peptide synthesis method. MP-B and analogue, [12-Ala]MP-B adopted an unordered structure in buffer solution. In the presence of neutral and acidic liposomes, the peptides took an α-helical structure. The two peptides interacted with neutral and acidic lipid bilayers. These results indicated that the hydrophobic α-helix of MP-B critically affected biological and helical content.
이병호,정징운,박홍재 인제대학교 1998 仁濟論叢 Vol.14 No.2
There are many manufacturing processes from gas contact and desulfurization sorbents present for desulfurization efficiency and economic improvement. It has been turned out to have many problems such as a lack of smooth supply, high purchasing cost of materials and high processing cost of desulfurization which has made it problematic for commercial use. Therefore the Korea Electric Power Corporation Research Center is in the middle of developing a dry-type high temp desulfurization system using domestic Limestone and Dolomite and carrying out a desulfurization study with shells in Japan. We are trying to develope the possible uses of desulfidation with after-use cow bone for recycling, practicability, and economic development. We obtained the calcination character of the samples by measuring weight variation according to thermal-decomposition with a thermal gravimetry analysis system and by observing variations in the phase. The first qualitative analysis of the sample resulted in a form of calcium phosphate hydroxide from cow bones and compared JCPDS files after obtaining a diffraction spectrum with an X-ray diffractometer. The second analysis let us ascertain the quality of cow bones.
이병호,정성욱,박홍재 인제대학교 1998 仁濟論叢 Vol.14 No.2
The 30 water samples from the Naktong River, Western Naktong River, and Suyoung Stream were collected, their nitrogen concentrations were analyzed and compared each other. The mean concentrations of NH3-N, NO2-N, and NO3-N in the water of Naktong River were 0.04, 1.47, and 0.93ppm, respectively. Those of Western Naktong River were 0.63, 1.17, and 1.43ppm, respectively and Suyoung Stream were 2.40, 1.19, and 1.38ppm, respectively. Therefore the mean concentrations of NO3-N were higher in the water of Western Naktong River than other two rivers and those of NH3-N and NO2-N have the highest concentration in the Suyoung Stream and Naktong River, respectively Above results suggested that the oxidation reaction was occurred easily in the Naktong River, The high NH3-N concentration in Suyoung Stream was supposed to be caused by the direct discharge of the living sewage.