H-Y 에 대한 단일클론 항체의 생산 및 그 이용에 관한 연구 1 . H-Y 에 대한 단일클론항체의 생산

심호섭(H . S . Shim),김재화(J . H . Kim),이병철(B . C . Lee),김종배(J . B . Kim),박홍양(H . Y . Park),정길생(K . S . Chung) 한국축산학회 1988 한국축산학회지 Vol.30 No.7

Testis supernatant, a source of H-Y, obtained from BALB/c mice was used to immunize females of same strain. B lymphocytes of mouse producing antibodies to H-Y were fused with SP2/0-Ag 14 myeloma cells and distributed to 384 wells of 96-well microtiter plates. Eighty hybridoma colonies were formed, resulting in 20.8 percent of fusion efficiency. Three strong positive wells from hybridoma colonies were selected for cloning by ELISA and two of them were also found to be positive by indirect immunofluorescence test. Twelve wells of ELISA-positive were selected after cloning and 2D45D4 clones from them were confirmed to produce monoclonal antibodies to H-Y by indirect immunofluorescence test.

생쥐수정란에 대한 H - Y 항체처리가 산자의 성비에 미치는 영향

심호섭,고정재,김종배,박홍양,정길생 ( H . S . Shim,J . J . Ko,J . B . Kim,H . Y . Park,K . S . Chung ) 한국축산학회 1986 한국축산학회지 Vol.28 No.12

These experiments were carried out to control the sex of offsprings in mice by sexing embryos using immunological means prior to transfer to pseudopregnant recipients. H-Y antisera were prepared in inbred SD female rats by repeated immunization of testis supernatant and spleen cells from males of same strain. ELISA and indirect immunofluorescence test were used to detect H-Y antibody in antisera. Eight- to 16-cell mouse embryos were cultured in medium with H-Y antibody and complement (treated embryos) and in medium with BSA (control embryos). After 24-48 hr of culture, embryos were observed their morphological characteristics under the phase contrast microscope. Embryos developed to normal blastocyst were transferred to pseudopregnant recipients and sex of resultant offspring was investigated. The results obtained in these experiments were summarized as follows: 1. Production of H-Y antibodies in antisera obtained from immunized rats was confimed by ELISA and indirect immunofluorescence test. 2. Of 270 embryos treated with H-Y antibody and complement, 126 embryos (46.7%) were developed to normal blastocysts. 3. Following transfer of 126 blastocysts, 16 embryos (12.6%) were survived to term and 13 females (81.3%), significantly high ratio of female offspring, were produced.

랜드레이스 계통조성돈의 유전적 및 표현형가적 변화추세

정영철(Y . C . Jung),김광주(G . J . Jeon),김성훈(S . H . Kim),박홍양(H . Y . Park),박영일(Y . I . Park),(M . F . Rothschild) 한국축산학회 1992 한국축산학회지 Vol.34 No.1

An animal model with the best linear unbiased prediction properties was used to evaluate the genetic trend of a Landrace herd. The Landrace base population was imported from Sweden and has been closed and linebred for 7 generations. The average number of parental animals per generation were 45 gilts and 15 boars and a total of 1,402 pigs were tested for performance. An average inbreeding coefficient of the 7th generation was 6.19%. The animal model used in the study included a complete additive genetic relationship matrix among all the pigs. The phenotypic trends for days to 90 ㎏ (DAYS 90), average daily gain (ADG), feed conversion rate (FCR). and selection index (INDEX) showed a favorable increase except backfat thickness (BF), but the increase was statistically insignificant. However, the genetic trends for the 5 traits were statistically highly significant (P$lt;0.01). The annual genetic gains with corresponding S.E. were .95±.14 days (.62%) for DAYS90, 9.97±1.73g (1.16%) for ADG, .008±.002㎝ (.56%) for BF, .003±.001% (.11%) for FCR, and 1.65±.14 (.95%) for INDEX, respectively. The values in the parentheses are the corresponding ratio of annual genetic change to phenotypic means.

동물생명공학 : 중합효소 연쇄반응을 이용한 돼지 내인성 레트로 바이러스의 검출과 분류

이동희 ( D. H. Lee ),이정은 ( J. E. Lee ),김훈미 ( H. M. Kim ),김계웅 ( G. W. Kim ),박홍양 ( H. Y. Park ),김영봉 ( Y. B. Kim ) 한국동물자원과학회 ( 구 한국축산학회 ) 2007 한국축산학회지 Vol.49 No.3

돼지의 산자수 및 체중에 대한 유전력 및 유전상관 추정

이병연,정선부,박홍양 ( B . Y . Rhee,S . B . Chung,H . Y . Park ) 한국축산학회 1987 한국축산학회지 Vol.29 No.1

This study was conducted to estimate the heritabilities, genetic, phenotypic and environmental correlations among litter size, body weights at birth and weaning in swine. The data analysed were recorded from 2794 male pigs produced by 282 boars of Hampshire, Yorkshire, Landrace, Duroc and Crossbreed raised at Duk Won-farm in Inchon from 1983 to 1984. The results obtained are summarized as follows: 1. The least square means over all litters studied were 9.86±0.13 pigs at birth, 8.57±0.13 pigs at weaning. Crossbreed Yorkshire and Landrace tended to be larger than Hampshire or Duroc in both traits. 2. The least square means of body weight over all litters studied were 1.37㎏ at birth, 6.82㎏ at weaning. 3. The hetitabilities of litter size at birth and weaning and body weight at birth and weaning were 0.454, 0.379, 0.009, 0.156, respectively. 4. Genetic correlation between litter size at birth and at weaning was 0.694, between body weight at birth and weaning was 0.294. 5. Phenotypic correlation between litter size at birth and at weaning was 0.794 and between body weight at birth and at weaning was 0.288. 6. Environmental correlation between litter size at birth and at weaning was 0.649, and between body weight at birth and at weaning was 0.294.

돼지의 경제형질에 대한 유전력의 추정

박동순,정선부,박홍양 ( D . S . Park,S . B . Chung,H . Y . Park ) 한국축산학회 1987 한국축산학회지 Vol.29 No.1

This study was conducted to estimate the heritabilities, genetic, phenotypic and environmental correlations among litter size, body weight at birth. weaning, daily gain and survival rate in swine. The data analysed were estimated on the basis of 4802 litters produced from 487 boars of Yorkshire, Hampshire, Duroc and Landrace raised at Duck-Won farm in Inchon from 1983 to 1985. The results obtained are summarized as follows: 1. The least square means over all litters studied were 9.86±0.10 pigs at birth, 8.24±0.10 pigs at weaning. 2. The least square means of body weight over all litters studied were 1.446±0.01㎏ at birth, 7,068±0.057㎏ at weaning. 3. The least square means over all litters studied were 0.188㎏ in daily gain, 85.1% in survival rate. 4. The estimates of heritabilities of litter size at birth and weaning, pig weight at birth and weaning, daily gain and survival rate were 0.286, 0.170, 0.110, 0.158, 0.183, 0.119, respectively. 5. Genetic correlation between litter size at birth and at weaning was 0.544, between body weight at birth and at weaning was 0.183 and between daily gain and survival rate was 0.026. 6. Phenotypic correlation between litter size at birth and at weaning was 0.493, between body weight at birth and at weaning was 0.163, and between daily gain and survival rate was 0.117. 7. Environmental correlation between litter size at birth and at weaning was 0.491, between body weight at birthand at weaning was 0.250; and between daily gain and survival rate was -0.040.

미꾸라지 ( Misgurnus mizolepis ) 초기배 발생의 공간적 시간적 분석

이재현(J . H . Lee),박홍양(H . Y . Park),이상호(S . H . Lee) 한국축산학회 1993 한국축산학회지 Vol.35 No.3

Early events of fertilization and embryonic development in fish are not well established as compared to those in other species despite the vast number of eggs available. With the advent of transgenic technology developed in mammalian species. There are great demand in the understanding of early embryonic development for efficient application of such technique to lish. We initiated a study to establish and exploit the developmental information occurring in loath. Temporal sequence was determined by inducing fertilization at 20±0.5℃. Perivitelline space formed within 5-10 min as evidenced by the lift-up of chorion upon fertilization. The first and second cleavage already occurred at 55 and 30 min in 20% of fertilized eggs and 36% of Z-cell embryos, respectively. Each cleavage took 25-30 min during subsequent development thereafter. Cytological analysis was also undertaken to demonstrate number of cells and localization of nuclei in an embryo. With this information the delay of embryonic development was successfully induced without loss of embryo survival at 15±20℃. The result indicates that embryonic development of loach is so rapid and may be delayed during the first cleavage for gene manipulation study.

고유지 옥수수 급여가 육성 비육돈의 도체특성 , 육질 , 돈육의 지방산 조성 및 콜레스테롤 함량에 미치는 영향

정정수,이재준,정영철,강정선,박홍양,김천제 ( C . S . Chung,J . J . Lee,Y . C . Jung,C . S . Kang,H . Y . Park,C . J . Kim ) 한국축산학회 1998 한국축산학회지 Vol.40 No.4

The current study was conducted to determine the effects of high oil com(HOC) on carcass traits and pork quality as well as fatty acid composition, and cholesterol concentrations of loin muscle. Total 104 pigs(Barrow; 52, Gilts; 52) were fed diets containing typical com plus animal fat(control) or high oil corn(HOC) replacing typical corn and animal fat for the final 85 days before slaughter. There were no differences in carcass traits and pork quality between pigs fed HOC diet and control diet. The proportion of polyunsaturated fatty acids of loin muscle and loin fat from pigs fed HOC-diets was higher than that of pigs fed by regular com diet(P$lt;0.05). The cholesterol concentration of loin muscle of HOC-diet pigs tended to be lower than that of the control diet pigs.

종돈의 능력검정에 미치는 성 및 환경요인의 효과

김계웅(G . W . Kim),장현기(H . G . Jang),박홍양(H . Y . Park) 한국축산학회 1995 한국축산학회지 Vol.37 No.2

This study was carried out in order to investigate the effects of sex, farrowing year, and farrowing month on the performance test for purebred swine. Data used in this study were 6,472 records of Hampshire, Landrace, Yorkshire and Duroc breeding stock which were registered at the herd book of Korea Animal Improvement Association. Their performance tests were conducted at the first and second Swine Performance Station from 1986 to 1992. Age at 30㎏ and 90㎏, daily gain, feed conversion, backfat thickness, and selection index were estimated according to sex, farrowing year, and farrowing month by Generalized Linear Model of SAS. The results obtained were summarized as follows; 1. In age at 30㎏, there were highly significant differences in the breeding females with the average of 75.41±1.10 days for the effect of sex, in 1992 with the average of 74.96±1.11 days for the effect of farrowing year, and the effect of farrowing month was significantly difference on December with the average of 74.48±1.12 days. In age of 90㎏, the effect of sex was significantly better in breeding male with the average of 142.98±1.52 days than in female, the effects of farrowing year and month were highly significantly best in 1992, and on December, respectively. 2. Daily gain of breeding males with the average of 915.14±13.77g was significantly superior to that of females. The effects of furrowing year and month were significantly superior in 1991 with the average of 918.29±14.07g, and on December with the average of 911.13±14.28g, respectively, for the daily gain. In feed conversion, there are highly significant differences between males and females, the effects of furrowing year and month are significantly superior in 1992 with the average of 2.38±0.02, and on December with the average of 2.55±0.02, respectively. 3. In backfat thickness, the effects of sex, farrowing year, and farrowing month were highly significantly superior in breeding males with the average of 1.58±0.03㎝, in 1992 with the average of 1.49±0.03㎝, and on October and December with the average of 1.56±0.03㎝, respectively. In selection index, the effects of sex, furrowing year, and furrowing month were highly significantly superior in breeding males with the average of 194.05±2.18, in 1992 with the average of 192.46±2.26, and on December with the average of 192.46±2.26, respectively.

종돈의 능력검정에 관한 연구 1 . 비육돈의 비육종료시 체중에 따른 산육능력의 비교

홍기창(K . C . Hong),윤덕영(D . Y . Yoon),박홍양(H . Y . Park) 한국축산학회 1989 한국축산학회지 Vol.31 No.6

This study was carried out in order to test the fattening performance and the change of body composition of pigs by slaughter weights. Sixty heads of three-way crossbred pigs were used for this purpose. Those pigs were slaughtered at live weight of 90, 100 and 110㎏, respectively. The results were summarized as follows: 1. In order to improve the feed requirement and backfat thickness in fattening pigs reared to 110㎏, the fattening performance of breeding pigs must be more improved. 2. As the slaughter weight increased, the carcass lengths were increased in both sexes. In the viewpoint of anatomy this shows the possibility of more attachment of muscles. 3. The percentages of dressing and lean cut were increased at 110㎏ slaughter weight and the proportion of ham was significantly increased than any other valuable parts. 4. But the quality of carcass was relatively lower than that of the developed countries. Therefore, it is desirable to select breeding pigs more intensively based on carcass quality. 5. Gross income per head was the highest at 110㎏ slaughter weight. But considering the turnover rate 90㎏ slaughter weight is acceptable under the present pork pricing system. 6. In order to increase pork export, the present system of grading and pricing should be reorganized. 7. Considering above-mentioned findings the present performance test of breeding pigs at 90㎏ should be changed to 110㎏ as soon as possible.