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인공산성비가 해바라기(Helianthus annuus) 잎에 미치는 영향
박홍덕,김성중 대구효성가톨릭대학교 자연과학연구소 1995 基礎科學硏究論集 Vol.9 No.-
Sunflower leaf was treated artifical acid rain(pH 2.8, 4.2 and 5.6) during the growing times to examine the effects of rain on chloroplast changes, chlorophyll content and chloroplast protein-complex patterns. The results obtained in this study were as follows : The chloroplast of rain exposed to secondary treatment was swollen in pH 2.8.At pH 4.2, envelope of chloroplast was partly swollen and has begun to lengthen in grana and lamellae. The content of chlorophyll were decreased in pH 2.8 and 4.2 after secondary treatment. LHCP band were disappeared after 1st and secondary treatment. CP I band were reduced after secondary treatment.
박홍덕 대구효성가톨릭대학교 자연과학연구소 1997 基礎科學硏究論集 Vol.11 No.2
To investigate the effects of 0.5% NaCl solution on structure of persimmon pelicarp 72 hrs, the morphological changes were observed with optical microscope. The results were as follows : In non-NaCl treatment, the part of sclereid began to separate after 12hrs and parenchyma cells were almost decayed after 72hrs. The part of parenchyma wall was decayed and tannins were free in the cytoplasm after 36hrs. In NaCl treatment, the part of sclereid was separated after 36hrs. After 72hrs parenchyma and the part of parenchyma wall were decayed and tannins were free in the cytoplasm.
박홍덕,김순동 대구효성가톨릭대학교 식품과학연구소 1997 식품과학지 Vol.9 No.-
김치논문에서의 배추에 대한 학명표기와 부위별 명명법이 매우 다양하며 통일된 표기법이 요망되었으며, 배추잎을 중록과 엽신으로 표기하기 보다는 백색부위와 녹색부위로 표기하는 것이 바람직한 것으로 판단되었다. 배추에서 백색부위의 도관에서는 환상, 계문상 및 망상비후로 구분되었다. 배추의 일반성분은 품종별로 상당한 차이가 있었으며, 세포벽 다당류의 함량도 품종간에 상당한 차이를 보였다. 배추의 세포벽 펙틴을 겔여과한 결과 4개의 피크로 분리되었으며 2개는 개략적인 분자량이 200백만 이상, 1개는 십만내외 1개는 1만이하를 나타내었다. 헤미셀룰로즈는 2백만과 1만으로 추정되는 2개의 픽크가 분리되었다. This study was conducted to examine the morphological characteristics and cell wall polysaccharides of Chinese cabbage(Brassica). First of all, the variety of scientific name and naming of parts of Chinese cabbage in the literatures of kimchi showed, which will unify marks. So, we propose not so much mid-rib and leaf blade of Chinese cabbage leaf as white part and green part, respectively. On the other hand, the forms of vessel elements of white part in Chinese cabbage consist in ring, sclariform and reticulate thickening. The proximate compositions and contents of cell wall polysaccharides of Chinese cabbage has significant differences between its cultivars. The cell wall pectin from Chinese cabbage exhibited four peaks with molecular weights of 2,000,000, about 100,000 and less than 10,000 by gel filteration chromatography. And hemicellulose did two peaks with molecular weights of 2,000,000 and 10,000.
암처에서 Putrescine이 시금치 (Spinacia oleracea L.) 잎의 노화에 미치는 영향
박홍덕 대구효성가톨릭대학교 자연과학연구소 1991 基礎科學硏究論集 Vol.5 No.-
Abstract The effect of putrescine(Put) , methylglyoxal-bis(guanylhydrazone)(MGBG) and Put+ MGBG on senescence in etiolated leaves of Spinacia of aleracea was investigated. The chlorophyll content was most delayed in 10-2M Put and 10-3 MGBG treatments. The retardation of soluble protein was promoted in non-treatment. The MGBG treatment was showed 4 major bands, but Put and Put+ MGBG treatments were not at 2 days. On the other hand, the MGBG treatment was showed several bands, but Put and Put + MGBG treatments were almost disappeared. Therefore, it was likely that Put and Put + MGBG treatments were lossed more in the protein content than MGBG.
박홍덕 대구효성가톨릭대학교 자연과학연구소 1998 基礎科學硏究論集 Vol.12 No.-
The structure of leaf in Pogonatum inflexum cormus was studied by electron microscope. The results indicate as follows: The differentiated lamella and grana wasn't observed but starch fain was single large-sized within the chloroplast. The membranous structure were observed with vesicular, multivesicular, concentric multivesicular and vesicle-tubular complex. These multivesicular bodies were originated from plasmalemma or endoplasmic reticulum. Key word : moss Cormus, leaf, structure
朴弘悳 대구효성가톨릭대학교 1982 연구논문집 Vol.25 No.1
Cedrus derdara(Roxb.) Loud의 종자, Glycine max Merr cv, Bean Sprouts의 胚軸上端, Helianthus annuus의 種子 및 Platanus orientalis의 잎에서 각각 callus를 誘導시켰다. 이들 callus에서 分離한 原形質體와 Allium fistulosum의 葉肉組織에서 分離한 原形質體를 각각 融合시켰다. The callus tissues were induced from seed of Cedrus derdara(Roxb.) Loud, shoot pole of hypocotyl of Glycine max Merr cv. Bean Sprouts, seed of Helinhus annus L., and leaf of Platanus arientalis. The protoplasts isolated from these callus tissues were fused with protoplast isolated from Allium fistulosum mesophyll. And the characteristics of tissue cultures used in this study were discussed.
시금치 엽육세포에서 분리한 Chlorplast Protein에 미치는 IAA 효과
朴弘悳 대구효성 가톨릭 대학교 1984 연구논문집 Vol.29 No.2
시금치 엽육에서 분리한 엽록체 단백질과 IAA 와의 관계를 광처리 하에서 조사하고, 엽록체 단백질에서 각 단백질의 band 를 SDS-polyacrylamide gel electroporesis 로 추정하였다. The relation between IAA and chloroplast protein isolated from spinach mesophyll was examined under the light. In addition, each protein bands in chloroplast protein were separated by SDS-polyacrylamide gel electrophoresis.
박홍덕 효성여자대학교 부설 한국환경위생연구소 1994 환경위생연구 Vol.4 No.1
배추 숙성중 뿌리잎의 형태학적인 변화에 대한 결과는 다음과 같다. 1. 백색부위의 표피세포는 24시간처리부터 붕괴되고, 유세포는 팽창하였다. 2. 녹색부위의 표피세포는 144시간, 유세포는 48시간처리부터 붕괴되었다. This study was conducted in morphological changes during matura-tion of baechu(Brassica compestris L. ssp.napsus var.pekinensis Makino) root-leaf. The result were as follows : 1. Epidermis and parenchyma cell of white region begin to breakdown and expansion from treatment for 24hr. 2. Epidermis and parenchyma cell of green region begin to breakdown from treatment 144hr and 48hr.
朴弘悳 대구효성가톨릭대학교 1986 연구논문집 Vol.33 No.1
The purpose of this study was carried out to optimal conditions for isolation of protoplasts, fusion, and culture from Glycine max callus and Solanum tuberosum mesophyll. The enzyme solutions of protoplasts isolation from Glycine max callus was 2% onozuka, 0.5% pectinase, and 0.5% driselase and incubation time was 8hr., but Solanum tuberosum mesophyll was 0.5% onozuka, 0.1% macerozyme and incubation time was 6hr. The fusigenic agent of protoplast was treated with 50% polyethylen glycol(PEG) 1,500 for 50 min. The fused protoplasts were cultured in protoplast growth medium supplemented with 1μm NAA and zeatin at 26℃ under dim light. Regeneration of few cell walls occured after 24hrs in culture, but callus was not obtained. And the characteristics of cultures used in this study were discussed.