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- 저자 : 박지현(Jee Hyoun Park) (검색결과 3 건)
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저삼투질 비이온성 조영제에 의한 신손상시 뇨중 N - Acetyl - β - D Glucosaminidase ( NAG ) 활성도의 의의
이준승(Jun Seung Lee),박지현(Jee Hyoun Park),곽귀철(Guee Chul Kwak),오동진(Dong Jin Oh),민철홍(Cheol Hong Min),강응택(Eung Taek Kang),유석희(Suk Hee Yu) 대한내과학회 1996 대한내과학회지 Vol.51 No.5
N/A Objectives: It has been claimed that the incidence of the contrast media induced nephrotoxicity (CMN) was reduced with the introduction of the nonionic low-osmolar contrast media (CM). The commonly used diagnostic tools for nephrotoxicity including serum creatinine levels, creatinine clearance and urine analysis cannot detect damage of renal proximal tubule in early stage. Urine N-Acetyl-β-D Glucosaminidase (NAG) is well documented a useful indicator for proximal tubular damage. Therefore we investigated incidence of nonionic low-osmolar CMN and whether the urine NAG activity could be used as a indicator of CMN. Methods: We studied 32 patients with normal renal functions (mean age: 58.7, M:F=23:9) and assayed their serum concentration of creatinine, urine NAG activities and SDS-PAGE profiles in the urine for 5 days after exposure to CM. Results: Mean creatinine level of 32 patients did not increase in comparison to their preexposure level on post exposure day 1, 3 and 5. But mean urine NAG activities increased significantly in comparison to their preexposure activities from day 1 to day 4 (p<0.05). Serum creatinine level increased either more than 50% or at least 1㎎/dL comparing the preexposure levels in 5 patients (15.6%) and did not in 27 patients (84.4%) and urine NAG activities increased from day 1 to day 4 in the former group and from day 1 to day 3 in latter group (p<0.05). Serum creatinine level increased either more than 20% or at least 0.3㎎/dL comparing the preexposure levels in 13 patients (40.6%) and did not in 19 patients (59.4%) and urine NAG activities increased from day 1 to day 3 in two groups (p<0.05). Serum creatinine level increased only 25% (sensitivity) of the patients with low molecular weight (LMW) protein in their urine SDS-PAGE and did not increased 90% (specificity) of the patients without LMW protein in their urine SDS-PAGE (50% or 1.0㎎/dL). The sensitivity and specificity of creatinine level were 33.3% and 55% (20% or 0.3㎎/dL) upon SDS-PAGE. Urine NAG activity revealed 76.9% of sensitivity and 52.6% of specificity. Conclusion: Nonionic low-osmolar contrast media, compared to ionic high osmolar contrast media, results in similar incidence of CMN and have the potential CMN subclinically. Urine NAG activity can be a useful indicator for CMN.
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Heat Shock Protein Expression in Endotoxin Treated Renal Epithelial Cells
Park, Jee-Hyoun,Oh, Dong-Jin,Yu, Soo Jeong,Lee,Tae-Woo,Kim, Cha-Hyun,Cho, Jae-Young,Yu, Suk-Hee,Kang, Eung-Taek 중앙대학교 의과대학 의과학연구소 2002 中央醫大誌 Vol.27 No.3-4
Recent studies have reported that markedly increased concentrations of a number of different classes of protein are initially identified by the marked increase in their concentration in cells after various stresses including heat shock. In this study, putative interactions between apoptosis and heat-shock proteins disturbed by endotoxin as aseptic shock model were investigated. The direct cellular damages were assessed by the LDH release from the cytoplasm of the human tubular epithelial cells(HK-2 cells) after lipopolysaccharides(LPS) treatment. And the expressions of Bcl2 and BAX mRNA that had been employed as an index of apoptosis to assess by what extent apoptosis contributed to tubular cell damage and the expression of heat shock protein(HSP)90, 72 and 27 with the Bcl2/BAX ratio in septic shock model were studied also. LPS treatment let HK-2 cells release SDH markely(14.33+/-1.55 vs. 61.00+/- 2.65, p<0.01). LDH release was greater significantly from the cells that had not been heat preconditioned than that of heat preconditioned(61.00+/- 2.65vs. 41.33 +/- 3.21,p<0.05) LPS treatment itself did not up-regulated Bcl2 significantly. But the expression of Bcl2 was marked with heat preconditioning. The expression of BAX was increased with LPS treatment but did not increase significantly with heat preconditioning(165% vs. 1496%). We did not see any contribution of heat preconditioning on the expression of HSP90. HSP72 expression was slightly increased with LPS treatment. But the expression of HSP27 was more evident after preconditioning with LPS treatment HSP27 expression was evident after LPS treatment. This study showed that the heat preconditioning was able to provide protection on the human tubular epithlial cells from LPS treatment extensively. The protection from cytolytic damage and increase of Bcl2/BAX ratio was closely related with the increase of HSP72 and HSP27, especially with LPS treatment.
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