초생추에서의 납의 독성과 철·구리·아연 및 단백질과의 상호작용

박전홍,김춘수,Park, Jun-hong,Kim, Chun-su 대한수의학회 1984 大韓獸醫學會誌 Vol.24 No.1

The protective effects of high levels of dietary iron, copper, zinc or protein on lead toxicity were studied In chicks. Growth retardation, reduction of feed intake, anemia and accumulation of lead in the bone and kidney were observed in chicks fed a diet containing 500mg lead as chloride per kg of feed for 42 days. Early changes due to ingested lead were inhibition of red blood cell ${\delta}$-aminolevulinic acid dehydrase at all doses and no effect of iron, copper, zinc or protein addition were observed. Tibia lead accumulation was reduced in chicks receiving additional dietary iron or zinc compared to the lead only group but increased in chicks given supplementary protein. Decreased body weight gain was overcome by supplementary zinc or protein in chicks fed lead but not by supplementary iron. Overall the results of this study show that lead poisoning can be partly reduced by providing supplementary iron, zinc or protein, but the interaction of these element remained to be elucidated.

Role of Prostaglandins in Ovulation in Rabbits

박전홍,권종국,Park, Jun Hong,Kwun, Jong Kuk The Korean Society of Veterinary Science 1979 大韓獸醫學會誌 Vol.19 No.2

Prostaglandin이 배란에 미치는 영향을 관찰하기 위하여 정상군과 양쪽 자궁을 제거한 가토에 indomethacin(prostaglandin 합성억제제)을 투여한 후 HCG데 의해 유발된 배란부위를 조직학적으로 관찰하였던 바 아래와 같은 결과를 얻었다. 1. indomethacin (10 mg/kg bw) 투여로 배란은 억제되었으며 투여량에 비례하여 배란은 억제되었다(p<0.01). 2. 자궁적출 단독으로는 배란에 영향을 주지 못하였다(p>0.05). 3. indomethacin은 난포파열을 억제함으로써 배란을 억제한다. 4. 토끼에서는 자궁이 아닌 장기에서 유래된 prostaglandin이 난포파열과정에 작용함으로써 배란을 시키는 작용이 있는 것으로 추정된다.

Effect of Indomethacin on Progesterone Profile in Rabbit

박전홍,권종국,Park, Jun-hong,Kwun, Jong-kuk The Korean Society of Veterinary Science 1985 大韓獸醫學會誌 Vol.25 No.1

배란 및 착상시기에 indomethacin 투여가 혈액의 progesterone 수준과 착상율에 미치는 영향을 조사하였다. Progesterone수준은 HCG 주사후 4시간이내에 9ng/ml로 증가 되었다가 배란시에는 3ng/ml로 감소하였다. indomethacin투여구에서는 배란시 8ng/ml를 나타내기 까지 계속증가하였다. Indomethacin투여는 착상을 억제하였다. 대조구의 착상율은 83%이나, indomethacin을 HCG 주사후 1일부터 4일까지 투여하면 착상율은 63%, HCG주사후 5일 부터 8일까지 투여하면 착상율은 27%로 감소하였다. Progesterone 수준은 HCG 주사후 12ng/ml의 높은 수준을 나타내다가 HCG 주사후 3일까지는 3ng/ml이하의 낮은 수준을 나타내였으며 4일 부터는 10ng/ml의 높은 수준을 나타냈다. Indomethacin을 HCG 주사후 5일부터 8일까지 투여하면 HCG 주사후 3일까지는 대조구와 비슷하지만 4일 부터는 5ng/ml이하로 대조구 보다 낮은 수준을 나타냈다.

초생추에서의 니켈의 독성과 아연·구리 및 납과의 상호작용

박전홍,김춘수,Park, Jun-hong,Kim, Chun-su 대한수의학회 1985 大韓獸醫學會誌 Vol.25 No.2

Nickel toxicity and interactions of nickel with zinc, copper, and lead were studied in glowing chicks fed supplemented diet. Feed intake and growth rate of the chick were reduced by 250mg nickel as a sulfate salt per kg of feed. The toxicity of nickel was decreased by zinc or copper supplementation, but not lead. High nickel feed increased nickel level in kidney and decreased zinc levels in tibia and plasma. However, low zinc levels in tibia and in plasma were reversed by zinc supplementation. Hemoglobin, packed cell volume, and aortic elastin content were increased in chicks fed nickel. These results suggest that nickel toxicity is induced by interference with zinc metabolism.

프로제스테론의 면역원 합성에 관한 물리화학적 연구

박전홍,권종국,Park, Jun-hong,Kwun, Jong-kuk 대한수의학회 1986 大韓獸醫學會誌 Vol.26 No.2

Progesterone immunogen has been synthesized and its melting point, Rf-value, UV and IR spectrum have been measured to develope the essential step in antisera production against low molecular weight substance. Mixed anhydride reaction was used to conjugate $11{\alpha}$-hydroxy-progesterone with succinic anhydride. Melting point of one intermediate compound was $156^{\circ}C$, and Rf-value was 0.41 in benzene : acetone : methanol (5 : 5 : 2). Maximum absorbance was measured at 242nm and ${\varepsilon}$ was $1.641{\times}10^4cm^2/mole$. Loss of hydroxy group was observed at 3450nm, and carbonyl group was appeared at 1160nm, 1250nm and 2960nm. These results indicated that the intermediate compound was progesterone hemisuccinate. Maximum absorbance of progesterone bovine-serum albumin(BSA) conjugate was observed at 250nm. Molar ratio of progesterone to BSA was average 15.4 on UV spectrum.

A study on decomposition of sulfonamide during meat processing

박전홍,Park, Jun-hong The Korean Society of Veterinary Science 1994 大韓獸醫學會誌 Vol.34 No.1

돈육의 가열과 냉동저장시 잔류된 설폰아미드가 증가 또는 감소되는 정도를 조사하였다. 설파메라진, 설파메타진, 설파모노메톡신, 설파디메톡신, 설파퀴녹사린을 돈육에 100ng/g씩 주사하고 30분간 $60^{\circ}C$로 가열하였을 때 설파메타진은 40%증가하였으나, $60-120^{\circ}C$로 가열한 다른 설폰아미드는 12-22% 감소하였다. 4주간 $-20{^{\circ}C}$이하로 냉동하였을 때, 설파메라진과 설파퀴녹사린은 각각 12%, 19% 감소하였으나 다른 설폰아미드는 변화가 없었다. 설폰아미드의 검출한계는 고상분산을 이용한 정제시 2.6-27ng/g이있으며, 액상분배를 이용한 정제시 25-36ng/g이었다. 본 실험의 결과 돈육중의 설폰아미드는 가열과 냉동저장에 따라 안정성이 변화될 수 있으며, 잔류물 정제방법으로는 고상분산과 액상분배방법을 이용할 수 있을 것으로 생각된다.

소의 혈청 비타민 E 농도와 결핍증에 관한 연구

이경갑,박전홍,임윤규,김희석,이창우,최희인,Lee Hyoung-kap,Park Jun-hong,Lim Yoon-kyu,Kim Hee-seok,Lee Chang-woo,Choi Hee-in 한국임상수의학회 1994 한국임상수의학회지 Vol.11 No.2

Serum $\alpha$-tocopherol was measured in cattle to evaluate normal range and to investigate the difference of $\alpha$-tocopherol levels between healthy cattle and diseased cattle. Seventy two heads of 1 year old beef Cattle have 429.9$\pm$77.2 $\mu\textrm{g}$/100ml of Serum $\alpha$-tocopherol. The Serum $\alpha$-tocopherol values of calves with diarrhea(8 heads), pneumonia(6 heads) and piroplasmosis(4 heads) were 87.1$\pm$19.2, 126.3$\pm$45.7 and 106.3$\pm$30.9$\mu\textrm{g}$/100ml, respectively. But that of calves in good health (S heads) was 357.t$\pm$68.4 $\mu\textrm{g}$/100m1. And the values of diseased calves are significantly lower an that of calves in good health(p<0.05). Seasonaly, serum $\alpha$-tocopherol levels of dairy Holstein cows were 529.9$\pm$ 120.3(March), 540.2$\pm$127.2(June), 566.9$\pm$149.5(September) and 550,0$\pm$ 125.4(December)$\mu\textrm{g}$/100m1, respectively. The values on autumn was the highest than that of othor seasons. Serum H-tocopherol level of cows with retained placenta was 262.2$\pm$40.6$\mu\textrm{g}$/100m1. And the level of retained placenta was significantly lower than that of healthy cattle regardless seasonal variation(p<0.05).

Effect of Artifitial Bleeding on Blood Pressure in Hypophysectomised or Neurohypophysectomised Rats

마츠무라 토미오,박전홍,권종국,Matsumura, Tomio,Park, Jun Hong,Kwun, Jong Kuk The Korean Society of Veterinary Science 1979 大韓獸醫學會誌 Vol.19 No.1

뇌하수체(腦下垂體) 전체(全體) 또는 뇌하수체후엽(腦下垂體後葉)만을 제거(除去)한 쥐에 있어서 방혈(放血)이 혈압(血壓)에 미치는 영향을 관찰하기 위하여 뇌하수체(腦下垂體) 또는 뇌하수체후엽(腦下垂體後葉)을 제거(除去)한 쥐에서 경동맥(頸動脈)을 통하여 체중(體重) kg 당(當) 10ml의 피를 뽑은 다음 physiograph 와 수은주(水銀柱)를 이용한 kymograph를 사용하여 혈압(血壓)을 측정하였던바 아래와 같은 결과를 얻었다. 1. 정상 쥐의 혈압(血壓) 및 hematocrit 값은 수컷이 $162{\pm}4mmHg$, $45{\pm}0.7%$이었고 암컷은 $157{\pm}5mmHg$, $34{\pm}0.6%$이었다(p>0.05). 2. 뇌하수체(腦下垂體)를 제거한 쥐의 혈압(血壓)은 $117{\pm}8mmHg$로서 정상($159{\pm}9mmHg$) 및 뇌하수체후엽(腦下垂體後葉)을 제거한 쥐의 혈압(血壓)($149{\pm}10mmHg$)보다 낮았다(p<0.001). 3. 뇌하수체(腦下垂體) 또는 뇌하수체후엽(腦下垂體後葉)을 제거한 쥐와 정상적(正常的)인 쥐에 있어서 방혈후(放血後)(10ml/kg BW) 혈압(血壓)은 50%이상 감소하였다(p<0.001).

소(牛) 식도구 평활근의 Adrenergic receptor 존재부위에 관한 연구

강동묵,조제열,박전홍,양일석,Kang, Tong-mook,Cho, Je-yoel,Park, Jun-hong,Yang, Il-suk 대한수의학회 1993 大韓獸醫學會誌 Vol.33 No.4

The preliminary studies on the localization of adrenoceptors were performed on smooth muscle strips of bovine esophageal groove. The mechanical activity of the muscle strip was recorded isometrically in vitro.w In the bottom circular muscle strips. the excitatory ${\alpha}-adrenergic$ responses were not blocked by tetrodotoxin$(2.1{\times}10^{-6}M)$ and denervation which was carried by cold storage of strips for 48 hrs in Tyrode's solution at $5-6{^{\circ}C}$ without oxygen supply. These excitatory ${\alpha}-adrenergic$ responses were partially blocked by atropine. In the lip longitudinal muscle strips, the inhibitory${\beta}-adrenergic$ responses were not blocked by pretreatment of tetrodotoxin and atropine. The results suggest that ${\beta}-adrenergic$ receptors mediating relaxations are located on the postsynaptic smooth muscle cells, whereas ${\beta}-adrenergic$ receptors mediating contractions are located both in the smooth muscle cells and in the cholinergic neurones.

축우 부루셀라병의 ELISA 진단법에 관한 연구

임윤규,이두식,박전홍,양기천,김승호,김공식,현관종,김우택,이영순,Lim, Yoon-kyu,Lee, Doo-sick,Park, Jun-hong,Yang, Ki-chun,Kim, Seung-ho,Kim, Kong-sick,Hyun, Kwan-jong,Kim, Woo-tack,Lee, Yong-soon 대한수의학회 1993 大韓獸醫學會誌 Vol.33 No.1

Enzyme-linked Immuno sorbent Assay (ELISA) for the serological diagnosis of Brucella abortus was developed and compared with plate agglutination test. Cell wall antigen was extracted from Brucella abortus 1119-3 by sonication and with a sodium deoxychlate solution. Optimum protein concentration of coating antigen were $0.4{\mu}g/100{\mu}{\ell}$ protein on each microtiter plate well. Horse radish peroxidase (HRP) labeled protein-G was used as a tracer of reacted antibodies. ELISA confirmed the agreeable results of 40 cases out of 43 cases by plate aggulutination test. ELISA diagnosed positive cases(10 out of 12) and negative cases (1 out of 12) with dubious sera by plate agglutination test. From this results ELISA could be used for the early diagnostic tools of Brucellosis in cattle.