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Pseudomonas acidovorns 의 5S rRNA 의 구조 결정
이승주,고문주,남정이,박인원 ( Seung Joo Lee,Moon Joo Koh,Jeoung E . Nam Shin,In Won Park ) 생화학분자생물학회 1990 BMB Reports Vol.23 No.1
The primary structure of the 5S rRNA isolated from Pseudomonas acidovorans was determined by enzymatic and chemical degradation methods. It consists of 115 nucleotides and contains no modified nucleosides. Based on Knuc values calculated from the homology of sequences of ten 5S rRNAs from Pseudomonas species, a phylogenic tree was reconstructed. There exists 60 to 87% homology between the 5S rRNA of P. acidovorans and those of other Pseudomonas species. The highest homology of sequences was found between 5S rRNA of P. acidovorans and that of P. cepacia; the phylogenic tree shows that these two species are evolutionarily very close. The secondary structure of the 5S rRNA of P. acidovorans was analyzed by enzymatic and chemical modification methods. The secondary structure determined shows that it is very similar to the generalized models proposed by other workers (De Wachter et al., 1982; Wolters and Erdmann, 1988; Koh, 1989). The secondary structure of 5S rRNA from P. acidovorans consists of five helices, five loops and two bulges.
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염주말의 핵에 있는 큰 소단위체 선구 rRNA 인트론의 구조
김동수,정연희,위호선,이승주,정태능,박인원,Kim, Dong-Soo,Joung, Yeon-Hee,Wee, Ho-Sun,Lee, Seung-Joo,Johng, Tae-Neung,Park, In-Won 생화학분자생물학회 1993 한국생화학회지 Vol.26 No.3
우리는 최근에 녹조류인 염주말의 핵에 있는 선구 rRNA가 인트론을 가지고 있다는 것을 보고한 바 있다. 우리는 여기서 이 선구 rRNA의 인트론이 시험관에서 자기이어맞추기를 하며, 이 인트론은 그 구조로 보아 I군 인트론의 B형에 속한다는 것을 보고하고자 한다. 이 인트론의 자기이어맞추기 반응은 겐타마이신 또는 L-아르기닌으로 억제된다. 이 언트론은 653개의 누클레오티드로 구성되어 있다. 우리는 인트론의 일차구조를 인트론을 함유하는 25S rDNA 부분의 염기 결합순서로부터 추정하였다. 인트론의 5' 말단의 염기는 시토신이고 그것과 연결된 5' 엑손의 3' 말단의 엽기는 우라실이다. 한편, 인트론의 3' 말단이 자기이어맞추기 자리를 선택할 때 형성하는 삼중복합체 구조로부터 추정한 인트론의 3' 말단의 엽기는 653번 위치에 있는 구아닌이고 그것과 연결된 3' 엑손의 5' 말단 염기는 우라실이다. 다른 I군 인트론들과 마찬가지로, 염주말의 선구 rRNA의 인트론은 Pl에서 P9까지 9개의 줄기를 가지며 4개의 보존된 연속부분 요소들을 다음과 갇은 순서로 함유하고 있다 :5'-P(GAAUGCGGGAGC)-Q(CCUCCGCAGG)-R(CCCCUAGAGACUAGA)-S(AUUCGAGAGUCCU)-3'. 이 인트론이 대부분의 다른 I군 인트론들과 주요하게 다른 점은 줄기 P6a의 3' 반쪽과 P6의 3' 반쪽 사이에 18개의 누클레오티드로 구성되는 가외의 줄기 P6c가 있다는 것과 줄기 P8이 open reading frame을 가지고 있고 크게 확대되어 있다는 것이다. Recently, we have reported that the nuclear large subunit pre-rRNA of the green alga Chaetomorpha moniligera has an intron. Now we report that the intron in this pre-rRNA self-splices in vitro and that its structure constructed shows that it is one of the group I B introns. The self-splicing reaction of this intron is inhibited by gentamycin or L-arginine. The intron consists of 653 nucleotides. The primary structure of the intron was derived from the sequence of a region of 25S rDNA that contains the intron. Its 5' terminal base is cytosine and 3' terminal base of the 5' exon that is linked to the intron is uracil. On the other hand, on the basis of tripartite structure that is proposed to form for the selection of 3' splice site, 3' terminal base of the intron has been inferred to be guanine on the position 653. The 5' terminal base of the 3' exon that is linked to this guanine is uracil. Like the other group I introns, the intron in pre-rRNA of Chaetomorpha moniligera contains nine base-paired stems P1 through P9, and it contains also four conserved sequence elements in the following order: 5'-P(GAAUGCGGGAGC)-Q(CCUCCGCAGG)-R(CCCCUAGAGA-CUAGA)-S(AUUCGAGAGUCCU)-3'. Major distinct features of this intron differing from most of the other group I introns are that it has an extra stem P6c which is formed between 3' half of the stem P6a and 3' half of the stem P6, and that the stem P8 has a long extension which contains an open reading frame.
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염주말의 핵에 있는 큰 소단위체 선구 rRNA 인트론의 구조
김동수,정연희,위호선,이승주,정태능,박인원 ( Dong Soo Kim,Yeon Hee Joung,Ho Sun Wee,Seung Joo Lee,Tae Neung Johng,In Won Park ) 생화학분자생물학회 1993 BMB Reports Vol.26 No.3
Recently, we have reported that the nuclear large subunit pre-rRNA of the green alga Chaetomorpha moniligera has an intron. Now we report that the intron in this pre-rRNA self-splices in vitro and that its structure constructed shows that it is one of the group I B introns. The self-splicing reaction of this intron is inhibited by gentamycin or L-arginine. The intron consists of 653 nucleotides. The primary structure of the intron was derived from the sequence of a region of 25S rDNA that contains the intron. Its 5` terminal base is cytosine and 3` terminal base of the 5` exon that is linked to the intron is uracil. On the other hand, on the basis of tripartite structure that is proposed to form for the selection of 3` splice site, 3` terminal base of the intron has been inferred to be guanine on the position 653. The 5` terminal base of the 3` exon that is linked to this guanine is uracil. Like the other group I introns, the intron in pre-rRNA of Chaetomorpha moniligera contains nine base-paired stems P1 through P9, and it contains also four conserved sequence elements in the following order: 5`-P(GAAUGCGGGAGC)-Q(CCUCCGCAGG)-R(CCCCUAGAGA-CUAGA)-S(AUUCGAGAGUCCU)-3`. Major distinct features of this intron differing from most of the other group I introns are that it has an extra stem P6c which is formed between 3` half of the stem P6a and 3` half of the stem P6, and that the stem P8 has a long extension which contains an open reading frame.
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Pseudomonas acidovorns의 5S rRNA의 구조 결정
이승주,박인원,고문주,남정이 생화학분자생물학회 1994 BMB Reports Vol.17 No.3
The primary structure of the 5S rRNA isolated from Pseudomonas acidovorans was determined by enzymatic and chemical degradation methods. It consists of 115 nucleotides and contains no modified nucleosides. Based on Knuc values calculated from the homology of sequences of ten 5S rRNAs from Pseudomonas species, a phylogenic tree was reconstructed. There exists 60 to 87% homology between the 5S rRNA of P. acidovorans and those of other Pseudomonas species. The highest homology of sequences was found between 5S rRNA of P. acidovorans and that of P. cepacia; the phylogenic tree shows that these two species are evolutionarily very close. The secondary structure of the 5S rRNA of P. acidovorans was analyzed by enzymatic and chemical modification methods. The secondary structure determined shows that it is very similar to the generalized models proposed by other workers (De Wachter et al., 1982; Wolters and Erdmann, 1988; Koh, 1989). The secondary structure of 5S rRNA from P. acidovorans consists of five helices, five loops and two bulges.
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