회음부를 침범한 근위형 유상피육종 - 증례보고 -

박용구,박호철,Park, Yong-Koo,Park, Ho-Chul 대한근골격종양학회 2001 대한골관절종양학회지 Vol.7 No.2

Epithelioid sarcoma has been well characterized as a distincitve entity. In the classic form, it occurs in the distal extremities of young adults. However, in the proximal form, it was found mostly in the pelvis, perineum, and genital tract of young to middle-aged adults. Morphologically, the tumor cells had promient epithelioid or rhabdoid features with marked cytologic atypia, and grew in a multinodular pattern. Immunohistochemically, cytokeratin, epithelial membrane antigen, and vimentin were positive in the tumor cells. This reports dealt with 32-year-old male lesion on his perineum. The tumor shows typical features of proximal type epithelioid sarcoma. We also discussed pathological differential diagnosis and prognosis on this particular disease. 유상피육종은 전형적인 형태인 경우에는 비교적 젊은 사람의 손이나 발과 같은 말단부에 호발하는 종양이다. 그러나 근위형의 경우는 주로 청장년층의 골반, 회음부 또는 생식기에 발생한다. 형태학적으로 종양세포는 뚜렷한 유상피 형태를 취하거나, rhabdoid형태를 보이며, 심한 세포학적 이형증과 함께, 다결절 형태로 성장한다. 면역조직화학적 검사에서는 특징적으로 cytokeratin, epithelial membrane antigen, vimentin이 양성이다. 본 보고는 32세 남자의 회음부에 발생한 근위형 유상피육종을 기술하였다. 형태학적으로 전형적인 근위형 유상피 육종의 조직학적 소견을 관찰하였으며, 병리조직학적 감별점과, 환자의 예후에 관하여 토론하였다.

수원포플러와 구아디 포플러 원형질체(原形質體) 융합(融合)에 의한 체세포잡종체(體細胞雜種體) 유도(誘導)

박용구,김정희,손성호,Park, Young Goo,Kim, Jung Hee,Son, Sung Ho 한국산림과학회 1992 한국산림과학회지 Vol.81 No.3

유망(有望) 속성수(速成樹)로 개발중(開發中)인 수원포플러(P. koreana ${\times}$ nigra var. italica)와 포플러 낙엽병(落葉病)에 내성(耐性)을 가진 구아디포플러(P. euramericana cv. Guardi)의 엽육조직(葉肉組織)에서 원형질체(原形質體)를 분리융합(分離融合)하여 잡종식물체(雜種植物體)를 생산(生?)하였다. 실험재료(實驗材料)인 수원포플러는 BA $0.5{\mu}M$, 구아디포플러는 BA $2.0{\mu}M$ 처리(處理)된 MS 배지(培地)에서 대량(大量) 증식(增殖)시킨 후 1/2 MS배지에서 계대배양(繼代培養)하여 전개(展開)된 엽조직(葉組織)을 사용(使用)하였다. 수원포플러는 효소용액(酵素溶液) I (Cellulase 2.0%, Macerozyme 0.4%, Hemicelluase 1.2%, Driselase 2.0%, Pectolyase 0.05%)에서 구아디포플러는 효소용액(酵素溶液) II (Cellulase 1.0%, Macerozyme 0.4%, Hemicellulase 1.2%, Driselase 2.0%, Pectolyase 0.05%)에서 원형질체(原形質體)를 분리(分離)하여 각각 $4.04{\times}10^7$, $2.45{\times}10^7$개의 높은 수율(收率)을 얻었다. 양수종간(兩樹種間)의 원형질체(原形質體) 융합율(融合率)은 PEG 40%가 포함(包含)된 융합용액(融合溶液)에 20분 처리(處理)하고 $Ca^{2+}$ 30mM 첨가(添加)된 희석액(稀釋液)(pH 10.5)을 사용(使用)하였을때 양수종간(兩樹種間) 1:1 융합율(融合率)이 30%정도로 높게 나타났다. 융합(融合)된 원형질체(原形質體)는 0.6M sucrose, $4.5{\mu}M$ 2, 4-D, $0.5{\mu}M$ BA가 첨가(添加)된 8p-KM에서 정치(定置) 혹은 진탕배양(震湯培養)하여 2개월후 캘루스를 얻을 수 있었으며, $5.0{\mu}M$ zeatin 처리구(處理區)에서 평균 4개의 식물체(植物體)가 재분화(再分化)되었다. 융합산물(融合?物)에서 유래(由來)한 식물체(植物體)의 잡종성(雜種性) 여부(與否)를 확인(確認)하기 위해 SDS-PAGE를 실시(實施)하였다. 모수(母樹)인 수원포플러와 구아디포플러간에는 단백질형에 차이(差異)가 있었으며 재분화(再分化) 개체중(個體中) 양친(兩親)의 중간형(中間型)을 나타내는 개체(個體)는 세포융합(細胞融合)에 의한 재분화개체(再分化個體)로 추정(推定)할 수 있었다. Protoplasts isolated from leaf mesophyll tissues of Populus koreana ${\times}$ P. nigra var. italica were fused with those of P. euramericana cv. Guardi. Well expended healthy leaves of 5 to 7 week-old-plantlet grown in vitro were used as source materials. Leaves from P. koreana ${\times}$ P. nigra var. italica and P. euramericana cv. Guardi were digested in enzyme solution I (2.0% Cellulase, 1.2% Hemicellulase, 0.4% Macrozyme, 2.0% Driselase, 0.05% Pectolyase ; w/v) and enzyme solution II (1.0% Cellulase, 1.2% Hemicellulase, 0.4% Macrozyme, 2.0% Driselase, 0.05% Pectolyase ; w/v), respectively, The highest frequency of fusion among the protoplasts originated from the two source materials was approximately 21% using 40% PEG or 15% dextran. In addition, fusion frequency was enhanced by incorporating 30mM of $Ca^{2+}$ in eluting solution at pH 10.5. Dividing cells and/or mint-calli were obtained by culturing the fusion products in a liquid 8p-KM medium supplemented with 0.6M sucrose, $0.45{\mu}M$ 2, 4-D, and $0.5{\mu}M$ BA. Shoots were regenerated from the fusion product-derived calli after culture on MS medium containing $5.0{\mu}M$ zeatin. To verify the putative hybrid or cybrid, SDS-PAGE was carried out. From the 24 regenerants, just two plants showed intermediate protein band patterns compared with those of the original source plants.

현사시나무 기내배양 엽육조직에서 분리된 원형질체 배양 및 식물체 재분화

박용구,손성호 ( Young Goo Park,Sung Ho Son ) 한국산림과학회 1988 한국산림과학회지 Vol.77 No.2

The leaf mesophyll protoplasts of Populass alba × glandrdosa were isolated from leaf of plantlet in vitro and cultured for plant regeneration. The MS medium (minus NH₄NO₃) with 0.5 ㎎/ℓ BAP and 2.0 ㎎/ℓ 2, 4-D showed the moderate frequency of dividing protoplasts cultured by the lipuid plating method during the first week of culture. The percentage of colony formation was revealed the highest frequency by the gauze contained semi-solid agar plating method after 5 weeks cultured. Ridding out the gauze, the micro-callus was formed on the same semi-solid medium in 8 weeks after protoplasts culture. For proliferation of callus, mini-callus was transferred on the MS solid medium with 0.5 ㎎/ℓ 2, 4-D and 0.1 ㎎/ℓ BAP 12 weeks after culture. Shoot regeneration occurred when the calli derived from protoplasts were cultured on MS medium with 1.0 ㎎/ℓ zeatin and such shoots could be readily rooted on the one half strengthen MS medium with non-phytohormone. Rooting shoots were planted in green-house 22 weeks after protoplast culture.

전단벽 구조물의 풍응답 저감을 위한 LRB의 적용

박용구,김현수,고현,김민균,이동근,Park, Yong-Koo,Kim, Hyun-Su,Ko, Hyun,Kim, Min-Gyun,Lee, Dong-Guen 한국공간구조학회 2011 한국공간구조학회지 Vol.11 No.1

일반적으로 전단벽은 횡력저항 요소로서 널리 이용되고 있다. 대부분의 전단벽 구조물은 통로의 목적으로 개구부를 필요로 하게 되고 전단벽들 사이가 슬래브나 연결보로 연결된 병렬전단벽의 형태를 띠게 된다. 본 연구에서는 병렬전단벽 구조물의 연결보 중앙부에 LRB(Lead Rubber Bearing)를 도입하였고 이 시스템의 풍응답 저감성능을 검토하였다. 제안된 방법의 효과를 살펴보기 위하여 20층 및 30층 예제구조물을 구성하였고 인공풍하중을 작성하여 경계비선형 시간이력해석을 수행하였다. 제안된 방법이 풍하중을 받는 고층 병렬전단벽 구조물의 사용성 향상에 도움을 줄 수 있는지 평가하기 위하여 일본 진동성능평가기준을 적용하여 보았다. 해석결과 본 논문에서 제안하는 LRB를 사용하여 병렬전단벽을 연결하는 방식이 풍응답 제어성능 개선에 효과가 있는 것을 확인할 수 있었다. In general, shear walls are employed as lateral resistance system. Most of shear wall structures require openings in shear walls and thus shear walls are linked by floor slabs or coupling beams resulting in the coupled shear wall structures. In this study, an LRB (lead rubber bearing) was introduced in the middle of the coupling beam of the coupled shear wall structures and the wind-induced response reduction effect of this system was investigated. In order to evaluate the control performance of the proposed method, 20- and 30-story building structures were used as example structures and boundary nonlinear time history analyses have been performed using artificial wind excitation. Japanese vibration evaluation criteria was employed to evaluate whether the proposed system could improve the serviceability of the tall coupled shear wall structures under wind excitation. Based on analytical results, it has been shown that the proposed method that connects shear walls with LRBs can improve the wind-induced response control effect.

삼차원 T형강체를 이용한 편심코어를 가진 전단벽-골조 구조물의 효율적인 지진해석모델 개발

박용구,이동근,김현수,Park, Yong-Koo,Lee, Dong-Guen,Kim, Hyun-Su 한국전산구조공학회 2013 한국전산구조공학회논문집 Vol.26 No.1

전단벽-골조 구조시스템의 구조적인 거동은 휨거동하는 전단벽과 전단거동하는 골조의 상호작용에 의하여 결정된다. 이러한 전단벽-골조 구조물의 거동특성을 효과적으로 고려하기 위하여 선행 연구에서는 2차원 T형 강체를 사용한 단순 해석모델을 제안하였다. 본 논문에서는 이를 바탕으로 편심코어를 가진 전단벽-골조 구조물에 대한 효율적인 해석모델을 제안한다. 2차원 등가모델을 3차원으로 확장하여 비틀림 거동을 고려할 수 있도록 하였고, 그 결과 제안하는 등가모델이 편심코어를 가지는 전단벽-골조 구조물에도 적용가능 하도록 하였다. In a shear wall-frame structural system, the structural response is determined by the interaction between the shear wall in bending mode and the frame in shear mode. In order to effectively consider these characteristics of a shear wall-frame structure, the simplified numerical model using the T-shape rigid body was suggested in the previous study. Based on the previously proposed model, an efficient numerical model for a wall-frame structure with an eccentric core has been proposed in this study. To this end, the previously proposed 2D model is extended to the 3D model and it is enhanced by considering torsion effects. As a result, the enhanced model can be applied to the analysis of a wall-frame structure with an eccentric core as well as a centric core.

이태리포푸라 Ⅰ-214 엽육조직에서 (葉肉組織) 원형질체 분리에 미치는 몇가지 요인

박용구,손성호 ( Young Goo Park,Sung Ho Son ) 한국산림과학회 1986 한국산림과학회지 Vol.74 No.1

A method isolating Populus euramericana cv. I-214 mesophyll protoplasts was developed to facilitate application of genetic engineering techniques to this species. The suitable medium for shoot multiplication in vitro was MS basal medium with 0.1 ㎎/ℓ BAP. The effects of several factors influencing protoplast isolation could be evaluated quickly by using leaf in vitro and known volumes of maceration and washing media. The best yields of mesophyll protoplasts were obtained using leaves in vitro in 2.0% Cellulase R-10, 0.8% Macerozyme R-10, 1.2% Hemicellulase, 2.0% Driselase, 0.05% Pectolyase Y-23, and O.6M Mannitol in addition to DTT and MES buffer adjusted to pH 5.6. Over 2.4 × 10^6 protoplasts per gram of leaf were produced using these conditions. For protoplast purification, the most favorable sucrose concentration of floating solution was 0.6M after washing them with CPW solution. This method of screening factors affecting protoplast isolation could be applicable to other species.

잣나무의 기내배양에 관한 연구 (Ⅰ) - Callus 의 유발과 생장 -

박용구,김우룡 ( Young Goo Park,Oue Ryong Kim ) 한국산림과학회 1983 한국산림과학회지 Vol.59 No.1

Embryo tissue from Pinus koraiensis was established in culture on G.D. and W.S. basal medium supplemented with the auxin (NAA, 2,4-D, IBA) and kinetin. The various combinations of growth regulators were studied in order to determine the specific requirements of the callus tissue in vitro. The inorganic nutrient combination of G.D. medium was found to be better than that of W,S. medium. G,D. basal medium supplemented with 0.1 ppm N.AA and 0.1 ppm kinetin was the most successful nutrient combination for the growth of callus induced from the embryo of P. koraiensis.

이차원 T형강체를 이용한 중심코어를 가진 전단벽-골조 구조물의 효율적인 지진해석모델 개발

박용구,이동근,김현수,Park, Yong-Koo,Lee, Dong-Guen,Kim, Hyun-Su 한국전산구조공학회 2013 한국전산구조공학회논문집 Vol.26 No.1

본 연구에서는 고층 전단벽-골조 구조시스템의 효율적인 해석모델을 제안하였다. 전단벽-골조구조시스템은 휨거동하는 전단벽과 전단거동하는 골조로 구성된다. 그리고 전단벽-골조구조시스템의 변형형상은 골조와 전단벽의 상호작용으로 결정된다. 효율적인 해석모델에서는 이러한 거동특성을 반영되어야 하므로 골조와 전단벽을 분리하여 동적인 거동특성을 반영할 필요가 있다. 본 연구에서는 벽체부와 골조부를 분리하기 위하여 T형 강체를 전단벽의 위치에 대체하는 방법을 사용하였다. 분리한 벽체부와 골조부 각각의 등가모델을 구성한 후 결합시키는 방법으로 고층 전단벽-골조구조시스템의 등가모델을 완성하였다. 제안한 등가모델의 정확성과 효율성을 검증하기 위하여 고층의 전단벽-골조 구조물의 시간이력해석을 수행하였고, 그 결과 제안한 등가모델이 해석시간과 컴퓨터 메모리를 현저하게 줄이면서도 정확한 결과를 도출하였다. In this study, an efficient analytical model for the dynamic analysis of tall buildings with a shear wall-frame structural system has been proposed. A shear wall-frame structural system usually consists of a core wall showing flexural behavior and a frame presenting shear behavior. Therefore, the deformed shape of the shear wall-frame structural system is shown by the combination of flexural mode and shear mode. These characteristics should be considered when an efficient analytical model is developed. To this end, the effect of shear wall and frame on the dynamic behavior of a tall building with a dual system has been separately investigated. In this study, the structural characteristics of a separated individual shear wall model and the frame model without shear wall has been evaluated. In order to consider the effect of the shear wall in the frame model without shear wall, a rigid body was used instead of the shear wall. Each equivalent model for the separated shear wall part and frame part has been independently developed and two equivalent models were then combined to create an efficient analytical model for tall buildings with a shear wall-frame structural system. In order to verify the efficiency and accuracy of the proposed method, time history analyses of tall buildings with a shear wall-frame system were performed. Based on analytical results, it has been confirmed that the proposed method can provide accurate results, requiring significantly reduced computational time and memory.

회원작품 - 순창 금과어린이집

박용구,Park, Yong-Gu 대한건축사협회 2016 建築士 Vol.2016 No.8

몇가지 삼림수종 (森林樹種) 종자발아에 (種子發芽) 대한 낙엽송엽 (落葉松葉) 추출물의 Allelopathic 효과

박용구,강군수,신동일 ( Young Goo Park,Goon Su Kang,Dong Ill Shin ) 한국산림과학회 1988 한국산림과학회지 Vol.77 No.1

Inhibitory effect of L. leptolepis fallen leaf extracts on the germination of Pinus densiflora, × Pinus rigitaeda, Pinus rigida, Pinus thunbergii and Larix leptolepis was investigated. Germination of those seeds in culture room and in pot at field showed the highly significant inhibitory effects by the aqueous extracts from L. leptolepis fallen leaves. Among them L. leptolepis was the most severely inhibited by the aqueous extracts used. To identify allelopathic substances, thin-layer chromatography was employed. Gallic, ferulic, t-cinnamic and vanillic acids were identified from fallen leaves of L. leptolepis. From the results, it is assumed that autotoxicity of L . leptolepis may act as inhibitory factors on germination of the species in natural stands.