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Bio-based Production and Upcycling of Polymers
Si Jae Park(박시재) 한국고분자학회 2021 한국고분자학회 학술대회 연구논문 초록집 Vol.46 No.2
Production of bio-based plastics has drawn much attention as one of the promising solutions to solve environmental problems such as global warming since renewable resources are used for the synthesis of these plastics in carbon-neutral manner. Also, complete biodegradable plastics have much potential to solve plastic wastes problems and microplastic problems, which are raised by improper disposal of non-degradable plastics. Biorefinery processes employing renewable resources for the production of chemicals, fuels and polymers have extensively been developed to produce carbon neutral target products in sustainable manner. For the development of biorefinery processes, engineered microorganisms have generally been employed as host catalysts to produce target products from renewable resouces. In this presentation, the strategies for bio-based production and upcycling polymers the development of are mainly discussed.
미니총설 : 바이오플라스틱 생산 미생물 플랫폼 제작을 위한 대사공학 전략 개발
박시재 ( Si Jae Park ),요키미코데이비드 ( Yokimiko David ),메리그레이스베일런 ( Mary Grace Baylon ),홍순호 ( Soon Ho Hong ),오영훈 ( Young Hoon Oh ),양정은 ( Jung Eun Yang ),최소영 ( So Young Choi ),이승환 ( Seung Hwan Lee ),이상엽 ( Sang 한국공업화학회 2014 공업화학 Vol.25 No.2
환경오염, 기후변화, 고갈되어가는 화석원료에 대한 문제를 해결하기 위해 재생가능한 자원으로부터 케미칼 및 고분자 등의 산업자원을 생산하는 친환경 공정개발에 많은 연구가 진행되고 있다. 최근에 재생가능한 바이오매스로부터 다양한 케미칼 및 고분자 등을 생산하는 바이오리파이너리 공정이 많은 관심을 받고 있으며, 석유화학기반산업을 보완 혹은 대체할 가능성이 매우 높은 친환경공정으로 생각되고 있다. 본 총설에서는 바이오리파이너리 공정에 핵심적인 촉매로 사용되고 있는 재조합 미생물의 개발의 최근 동향을 바이오나일론, 바이오폴리에스터의 생산을 위하여 개발되고 있는 재조합 미생물의 대사공학전략을 중심으로 살펴보고자 한다. As the concerns about environmental problems, climate change and limited fossil resources increase, bio-based production of chemicals and polymers from renewable resources gains much attention as one of the promising solutions to deal with these problems. To solve these problems, much effort has been devoted to the development of sustainable process using renewable resources. Recently, many chemicals and polymers have been synthesized by biorefinery process and these bio-based chemicals and plastics have been suggested as strong candidates to substitute petroleum-based products. In this review, we discuss current advances on the development of metabolically engineered microorganisms for the efficient production of bio-based chemicals and polymers.
재조합 대장균에서 MaoC를 이용한 지방산으로부터의 중간사슬길이 폴리하이드록시알칸산 생산 연구
박시재(Si Jae Park),이승환(Seung Hwan Lee),오영훈(Young Hoon Oh),이상엽(Sang Yup Lee) 한국생물공학회 2014 KSBB Journal Vol.29 No.4
Biosynthesis pathway of medium-chain-length (MCL) polyhydroxyalkanoates (PHA) from fatty acid β-oxidation pathway was constructed in recombinant Escherichia coli by introducing the Pseudomonas sp. 61-3 PHA synthase gene (phaC2) and the maoC genes from Pseudomonas putida, Sinorhizobium meliloti, and Ralstonia eutropha. The metabolic link between fatty acid β-oxidation pathway and PHA biosynthesis pathway was constructed by MaoC, which is homologous to P. aeruginosa (R)-specific enoyl-CoA hydratase (PhaJ1). When the E. coli W3110 strains expressing the phaC2 gene and one of the maoC genes from P. putida, Sinorhizobium meliloti, and Ralstonia eutropha were cultured in LB medium containing 2 g/L of sodium decanoate as a carbon source, MCL-PHA that mainly consists of 3-hydroxyhexanoate (3HHx), 3-hydroxyoctanoate (3HO) and 3-hydroxydecanoate (3HD), was produced. The monomer composition of PHA and PHA contents varied depending on MaoC employed for the production of PHA. The highest PHA content of 18.7 wt% was achieved in recombinant E. coli W3110 expressing the phaC2 gene and the P. putida maoC gene. These results suggest that MCL-PHA biosynthesis pathway can be constructed in recombinant E. coli strains from the b-oxidation pathway by employing MaoC able to supply (R)-3-hydroxyacyl-CoA, the substrate of PHA synthase.
Deinococcus radiodurans 유래 DR1558과 PprM에 의한 Corynebacterium glutamicum의 라이신 생산 향상 연구
김수미 ( Su-mi Kim ),임상용 ( Sangyong Lim ),박시재 ( Si Jae Park ),주정찬 ( Jeong Chan Joo ),최종일 ( Jong-il Choi ) 한국미생물생명공학회(구 한국산업미생물학회) 2017 한국미생물·생명공학회지 Vol.45 No.3
The expression of Deinococcus radiodurans dr1558 and pprM genes was examined for enhanced lysine production in recombinant Corynebacterium glutamicum. These genes are known to confer high tolerance to pH and osmotic shock in Escherichia coli. D. radiodurans dr1558 and pprM genes were expressed in C. glutamicum by using 6 synthetic promoters of different strengths, to evaluate the effect of expression efficiency on lysine production. Recombinant C. glutamicum expressing DR1558 under the L26 and I64 promoters showed higher lysine production than that expressing DR1558 under other promoters. Similarly, recombinant C. glutamicum expressing PprM under same promoters (L26 and I64) showed a higher increase in lysine production compared to that expressing PprM under other promoters. In the absence of CaCO<sub>3</sub> in the medium, the expression of DR1558 or PprM also increased lysine concentration in C. glutamicum depending on the promoter used. Together, these results suggest that genes involved in radiation tolerance in D. radiodurans can be used to enhance production of amino acids and their derivatives.
오영훈 ( Young Hoon Oh ),이승환 ( Seung Hwan Lee ),박시재 ( Si Jae Park ) 한국공업화학회 2014 공업화학전망 Vol.17 No.3
온실가스축적으로 인한 지구온난화를 비롯한 기후변화 및 고갈되어가는 석유를 비롯한 화석원료에 대한 문제를 해결하기 위해 재생가능한 자원으로부터 바이오기반 케미칼 및 고분자 등의 화학제품을 생산하는 바이오화학 공정이 많은 관심을 받고 있다. 본 기고문에서는 바이오화학공정에 핵심적인 촉매로 사용되고 있는 재조합 미생물 및 효소의 최근 개발동향을 내열성 엔지니어링 플라스틱인 바이오나일론의 생산을 위하여 개발되고 있는 바이오촉매를 중심으로 살펴보고자 한다.
ε-Caprolactam과 2-Piperidone으로부터 나일론 6,5 공중합체 제조 및 특성평가
김혜영(Hye Young Kim),고진선(Jin Seon Goh),류미회(Mi Hee Ryu),김대수(Dae Su Kim),송봉근(Bong Keun Song),이승환(Seung Hwan Lee),박시재(Si Jae Park),제갈종건(Jong Geon Jegal) 한국고분자학회 2014 폴리머 Vol.38 No.1
본 연구에서는 바이오매스 기반 나일론 6,5 공중합체를 제조하기 위하여 단량체인 ε-caprolactam과 2-piperidone을 glucose로부터 발효공정으로 제조된 lysine과 5-aminovaleric acid로부터 각각 제조하였다. 이들을 potassium tertbutoxide를촉매로 하고 acetyl-2-caprolactam과 이산화탄소를 개시제로 사용하여 40 oC에서 음이온 개환 중합 방법을 이용하여 나일론 6,5 공중합체를 제조하였다. 제조된 바이오 나일론 6,5 공중합체의 특성을 여러 가지 기기분석방법으로 분석하였다. 이때 얻어진 고분자의 점도분자량(Mη)은 최대 30000 g/mol 정도였으며, 중합 수율은 50% 이상이었다. 이들은 모두 semi-crystalline 고분자로 밝혀졌다. 열 특성 분석 결과 용융온도는 약 165 oC 정도로 분해온도 250 oC와 큰 차이를 나타내었다. 이들 고분자들은 우수한 가공성과 응용성을 지닐 것으로 예상된다. To prepare biomass based nylon 6,5 copolymers, ε-caprolactam and 2-piperidone, the monomers of nylon 6,5copolymers, were synthesized respectively from lysine and 5-aminovaleric acid which were produced from glucose bythe fermentation process. The copolymers were then polymerized by the anionic ring opening polymerization of them at40 oC, using potassium tert-butoxide as a catalyst and acetyl-2-caprolactam and carbon dioxide as initiators. The preparedcopolymers were characterized with various analytical methods: their viscosity molecular weight (Mη) was as high as30000 g/mol and polymerization yield was over 50%, and it was found that they were semi-crystalline polymers havingmelting point at 165 oC which was much lower than its thermal degradation point, 250 oC. These polymers were expectedto have good thermal processability and application fields.