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Alcaligenes latus 의 배양방법에 따른 생분해성 플라스틱의 생산특성
조경숙,최희식,류희욱,조강현,박성연 ( Kyung Suk Cho,Hee Sik Choi,Hee Wook Ryu,Kang Hyun Cho,Seong Yeon Park ) 한국환경생물학회 1998 환경생물 : 환경생물학회지 Vol.16 No.1
Optimum conditions for cell growth and poly (3-hydroxybutyrate, PHB) biosynthesis of Alcaligenes latus. which can accumulate PHB simultaneously in associated with cell growth and grows well on a cheap carbon source such as sucrose, were investigated. The effect of initial sucrose concentration, ranged from 10 to 50g/L, on the cell growth and PHB accumulation were studied by a batch culture in a 5 L fermentor. PHB productivity was increased from 0.11 to 0. 36 g-PHB/L·h with increasing sucrose concentration up to 30g/L, and it was fairly constant between concentrations of 30g/L and 50g/L. The maximum specific growth rate, 0.44h^-1, was obtained at 30 g-sucrose/L, and the growth was inhibited at concentrations of sucrose over 30g/L. A. latus was also cultivated to attain high PHB productivity by fed-batch or semicontinuous fed-batch culture techniques. PHB productivity by a fed-batch culture under phosphate limitation (1.14g-PHB/L·h) were smaller than that without nutrient limitation (2.10g-PHB/L·h), but there is no significant difference of PHB content (55-60%) obtained in both culture. By a semicontinuous fed-batch culture without nutrient limitation, high PHB productivity could be obtained as 4.5∼4.8 g-PHB/L·h. The average PHB content, the yield of dry cell weight, and the yield of per consumed sucrose were 60%, 0.48, and 0.32, respectively. These results suggest that the semi continuous fed-batch culture technique is one of promising methods to achieve high PHB productivity by A. latus.
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Alcaligenes latus의 배양방법에 따른 생분해성 플라스틱의 생산특성
박성연,조강현,최희식,류희욱,조경숙 이화여자대학교 환경문제연구소 1998 이화환경연구 Vol.2 No.-
생장과 동시에 poly(3-hydroxybutyrate, PHB) 를 체내에 축적하고, 값싼 당류인 sucrose를 이용할 수 있는 Alcaligenes latus의 생장과 PHB 생산을 최적화 할 수 있는 배양 조건에 대하여 연구하였다. 균주 생장과 PHB 생산성에 미치는 sucrose의 초기농도의 영향을 5L 발효조를 이용하여 회분식 배양 방법에 의해 조사하였다. PHB생산성은 sucrose농도가 10g/L에서 30g/L로 증가함에 따라 0.11에서 0.36g-PHB/L·h까지 증가하였고, 그 이상의 농도에서는 큰 변화없이 0.36∼0.39g-PHB/L·h에서 일정하게 유지되었다. 균주의 비증식속도는 sucrose 농도 증가함에 따라 증가하여 30g/L에서 최대값 0.44h()을 보였으며, sucrose의 농도가 30g/L을 초과하면 균주의 생장은 기질에 의해 저해 받았다. A. latus를 이용하여 대량으로 PHB를 생산하기 위해 유가식 배양과 반연속 유가식 배양을 수행하였다. 인산염 제한하에서 유가식 배양에 의해 얻은 PHB 생산성은 1.14g-PHB/L·h로, 인산염 제한없는 조건에서 얻은 PHB생산성(2.10g-PHB/L·h)에 비해서 훨씬 작았다. PHB함량은 영양소 제한에 관계없이 55∼60%이었다. 영양소 제한 없이 반연속 유가식 배양법에 의해 PHB생산성을 4.5∼4.8g-PHB/L·h까지 향상시킬 수 있었다. 평균 PHB 함량, 소비된 sucrose당 건조 균체중량 수율 및 PHB 생산 수율은 각각 60%, 0.48 및 0.32이었다. 그러므로, 생장과 동시에 PHB를 축적할 수 있는 A. latus를 이용하여 PHB를 생산할 경우, 반연속 유가식 배양법은 높은 PHB생산성을 얻을 수 있는 유망한 배양법임을 알 수 있다. Optimum conditions for cell growth and poly(3-hydroxybutyrate, PHB) biosynthesis of Alcaligenes latus, which can accumulate PHB simultaneously in associated with cell growth and grows well on a cheap carbon source such as sucrose, were investigated. The effect of initial sucrose concentration, ranged from 10 to 50g/L, on the cell growth and PHB accumulation were studied by a batch culture in a 5L fermentor. PHB productivity was increased from 0.11 to 0.36g-PHB/L·h with increasing sucrose concentration up to 30g/L, and it was fairly constant between concentrations of 30g/L and 50g/L. The maximum specific growth rate, 0.44h(), was obtained at 30g-sucrose/L, and the growth was inhibited at concentrations of sucrose over 30g/L. A. latus was also cultivated to attain high PHB productivity by fed-batch or semicontinuous fed-batch culture techniques. PHB productivity by a fed-batch culture under phosphate limitation(1.14g-PHB/L·h) were smaller than that without nutrient limitation(2.10g-PHB/L·h). but there is no significant difference of PHB content(55∼60%) obtained in both culture. By a semicontinuous fed-batch culture without nutrient limitation, high PHB productivity could be obtained as 4.5∼4.8g-PHB/L·h. The average PHB content, the yield of dry cell weight, and the yield of per consumed sucrose were 60%, 0.48, and 0.32, respectively. These results suggest that the semicontinuous fed-batch culture technique is one of promising methods to achieve high PHB productivity by A. latus.
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