새우젓 중의 단백질분해효소에 대한 연구

박길홍(Gil Hong Park),주진순(Jin Soon Ju) 韓國營養學會 1986 Journal of Nutrition and Health Vol.19 No.6

Restriction Fragment Length Polymorpohisms 분석을 이용한 진행성 근이양증의 보인자 검색

박길홍(PARK Gil Hong),박선화(PARK Sun Hwa) 대한체질인류학회 1991 해부·생물인류학 (Anat Biol Anthropol) Vol.4 No.1

진행성 근이양증을 가진 두 가족을 대상으로 혈청 creatine kinase치 측정과 더붙어 Restrietion Fragment Length Polvmorphi는(RFLPs) linkage 분석을 통해 보인자 검출을 시행하였다. probe는 진행성 근이양증 유전자내 위치한 것으로서 pERT 87 1 (Xmn 1), pFRT 87 8(Taq 1. Bst XI), pERT 87.15(Xmn 1, Bam HI), 근접한 prode로서 D2(Pvu Ⅱ) 및 pXUT23(B히 Ⅱ)dmf 사용하였다. 진단 대상 6명중 2명은 각각 97.1%, 99.9% 확률로 보인자였고, 4명은 99.9% 확률로 정상임을 진단하였다. 이상의 결과로 미루어 RFLPs linkage 분석법은 진행성 근이양증 뿐아니라 다른 X-염색체 열성 유전병에도 적용되어 대아 진단과 보인자 검색에 보다 정확한 진단법이 될 것으로 사료된다.

The Stability of p53 in Ras-mediated Senescent Cells in Response to Nucleolar Stress

Choong-Ryoul Sihn(신충렬),Gil Hong Park(박길홍),Kee-Ho Lee(이기호),Sang Hoon Kim(김상훈) 한국생명과학회 2009 생명과학회지 Vol.19 No.4

B23/nucleophosmin은 핵인 단백질로서 외부 스트레스에 의해 핵인에서 핵으로 이동하게 된다. 이러한 세포내 위치변화는 MDM2에 의한 p53단백질의 안정화에 영향을 미친다. 노화세포는 거대한 단일 핵인을 가지고 있으며, 외부 스트레스에 의해 p53 안정성이 감소한다. 그렇지만, 노화세포에서 어떠한 기전에 의해 p53의 불안정성이 증가하는 지는 아직 밝혀진 바가 없다. 따라서 본 연구에서는 노화세포에서 B23/nucleophosmin과 p53간의 상호 관련성을 조사하여 p53 안정성에 미치는 영향을 규명하고자 하였다. 본 연구에서는 IMR90세포주에 ras oncogene을 과발현시켜 노화세포를 유도하였다. 핵인 스트레스에 의해 노화세포 내 p53 단백질 발현은 감소하였으나, B23/nucleophosmin 단백질의 발현은 정상세포와 큰 차이가 없었다. 그렇지만, 두 단백질의 세포 내 위치는 노화세포에서 변화가 있었다. 즉, 정상세포와 달리, 노화세포에서는 스트레스에 의해 핵 내 p53발현이 증가하지 않았으며, B23/nucleophosmin은 핵 내로 이동하지 않고, 핵인에 그대로 머물러 있었다. 노화세포에서 MDM2와 p53간 상호결합이 안정적으로 유지된대 비하여, p53과 B23/nucleophosmin간의 상호결합은 감소하였다. 이러한 결과는 노화세포에서 핵인 스트레스에 의한 p53단백질의 안정성은 B23/nucleophosmin 결합이 감소하여 일어나는 것으로 해석된다. B23/nucleophosmin, a nucleolar protein, translocates into the nucleus from the nucleolus when cells are damaged by extracellular stresses. Recently, it was shown that such translocation of B23/nucleophosmin in normal fibroblasts under stress conditions increases both the stability and activation of the p53 protein by disrupting its interaction with MDM2. Senescent cells have a single large nucleolus and a diminished capacity to induce p53 stability upon exposure to various DNA damaging agents. To investigate the role of B23/nucleophosmin in p53 stability in senescent cells, we established a senescence model system by expressing the ras oncogene in IMR90 cells. The stability of p53 was reduced in these cells in response to nucleolar stress, although the level of B23/nucleophosmin protein was not changed. In addition, p53 did not accumulate in the nucleus and B23/nucleophosmin did not translocate into the nucleoplasm. The binding affinity of B23/nucleophosmin with p53 was reduced in senescent cells, whereas the interaction between MDM2 and p53 was stable. Taken together, the stability of p53 in ras-induced senescent cells may be influenced by the ability of B23/nucleophosmin to interact with p53 in response to nucleolar stress.

민감도가 향상된 링 형태의 비 침습식 혈당 센서

이기혁,이국주,이동호,김문일,박길홍,Lee, Ki-Hyuck,Lee, Kook-Joo,Lee, Dong-Ho,Kim, Moon-Il,Park, Gil-Hong 한국전기전자학회 2007 전기전자학회논문지 Vol.11 No.4

루프 공진기 구조를 가지는 비침습식 혈당 센서 구조를 제작하여 혈당 측정을 위한 민감도 측정을 하였다. 0%와 20%의 글루코스 용액의 전기적 특성을 확인하고, 25mm${\sim}$170mm의 지름을 가지는 링 구조를 이용하여 식염수 중 글루코스 농도에 따른 반사 손실의 차이를 측정하였다. 링 형태 프로브가 혈액과 같은 인체 내부의 물질을 측정하는데 종단 개방 프로브에 비해 유리한 특성을 가지며, 측정 주파수 중 가장 낮은 285MHz에서 S11이 0.94의 차이를 보여, 저주파일수록 민감도가 향상됨을 알 수 있었다. A novel sensitive and non-invasive ring-type glucose probe was designed and measured. The magnitude and phase of return loss change with different concentration of the glucose solution inside the loop structure. Ring type probe is more advantageous than open ended probe to measure electrical characteristics under the skin. The maximum difference of return loss was 0.94 for the concentration difference of 0% and 20% at 285MHz and more sensitive in low frequency.

방사성동위원소 표지 치료용 올리고뉴클레오티드를 이용한 나노핵폭파 유전자 치료법 소개

최재걸(Jae Gol Choe),이희영(Hee Young Lee),박길홍(Gil Hong Park),류총근(Chong Kun Ryu),김명곤(Meyoung Kon Kim) 대한핵의학회 2001 핵의학 분자영상 Vol.35 No.3

N/A

모발생장기 유도 C3H생쥐에 있어서 미녹시딜과 생약추출 혼합 조성물의 모발 재성장 유도 효능

이계호(Kye Ho Lee),한선일(Sun Il Han),박길홍(Gil Hong Park),권영이(Young Ee Kwon) 대한약학회 2003 약학회지 Vol.47 No.1

The hair cycle consists of three phases, growth (anagen), involution (catagen) and quiescence (telogen) phases. In order to evaluate hair re-growth effect of herbal extracts mixture containing the 70% ethanol extracts of Polygoni Mulitiflori Radix, Mori Cortex Radix, Gingo Biloba Folium and Pine bud, we have examined the induction of the anagen phase and elongation of the anagen period using C3H mice. MorPhological examination was done by Hattori and Ogawa's method. Enzyme activites of γ-glutamyl transpeptidase (γ-GT) and alkaline phosphatase(ALP) was detected by Bessey-Lovry-Brock's method. Enzyme activity as a biochemical marker of cycle was investigated in the third hair cycle period of C3H mice after depilation. 3% Milnoxldll treated group and herbal extract mixture treated group were shown 3 days earlier initiation of anagen than control group. In cycling mousse skin, γ-GT activity is pronounced during anagen and greatly diminished during telogen. Herual extract mixture has shown promising hair re-growth efect on hair follicula cycles of C3H mice.

전기자극이 건의 치유에 미치는 영향에 관한 실험연구

홍성철,김덕래,김태연,정전은,박길홍 大韓成形外科學會 1991 Archives of Plastic Surgery Vol.18 No.1

The mechanism of tendon healing has been a controversial issue. Although there seems to be a dipolarizing opinion regarding the tendon healing, it is well known to us that the tendon has both intrinsic and extrinsic healing capacity which has proved in vivo and in vitro studies. Tendon adhesion is a common sequella after tendon repair, particularly after the ling period of immobilization. This can be explained by one wound concept or extrinsic tendon healing. If we can modify the natural healing process of the tendon by augmenting the intrinsic capacity, we can reduce the period of immobilization to allow early motion for better gliding. in this study, we used the electrical stimulation technique, which has been known to have good growth stimulatory effect on the living cells, to investigate its effect on tendon healing in organic culture for 6 weeks. Total 64 flexor tendons were harvested from 8 rabbit paws and they were cultured in separate petri-dishes.: 32 tendons were used for the control group and the rest 32 were used for the electric stimulation group. Tendons through which a continuous 7 μAmp current was passed at the repair site were compared with the non stimulated controls. In reference to the histologic study with Hematoxylin ?? Eosin stain and van Gieson stain, the following results were obtained. 1. There was found no necrosis in the specimens of the control as well as the elctrical stimulation group during 6 weeks' culture period. 2. Starting from 1 week, there was prominent increase of fibroblastic proliferation and collagen production in electrical stimulation group and these active repair processes persisted up to 6 weeks. Accordingly, I conclude that the intrinsic tendon healing capacity can be enhanced by the electrical stimulation in vitro.

반복횡하중을 받는 고강도 철근콘크리트 기둥의 전단내력에 관한 연구 : 에폭시 보강철근을 사용하여 using epoxy-coated reinforcing bar

박길홍,김제원,설광욱,송창영,이수곤 대한건축학회 2000 대한건축학회 학술발표대회 논문집 - 계획계/구조계 Vol.20 No.1

진행성근이양증중 Gonadal mosaicism을 나타내는 가계의 유전자분석과 산전진단에 관한 연구

박길홍,김병호,류총근 최신의학사 1994 最新醫學 Vol.37 No.5

Protein Kinase C와 S6 Kinase에 關한 硏究

朴吉洪,黃祐翊 고려대학교 의과대학 1990 고려대 의대 잡지 Vol.27 No.1

To investigate the role of protein kinase C(PKC) and S6 kinase in the signal transduction of the factors which exhibit the growth promoting activity including serum growth factors, phorbol-12-myristate-13-acetate(PMA) and insulin. The enzymes were purified and characterized from human colon cancer cell, HRT-18. Meanwhile, biosynthetic labelling of phosphoproteins were performed with [³²P]H₃PO₄ in HRT-18 and mouse embryo fibroblast(MEF) under the influence of epidermal growth factor(EGF), fetal bovine serum(FBS), PMA and insulin, Furthermore, in vivo substrates of PKC and S6 kinase were observed in nucleus, mitochondria, cytosol and ribosome fractions of HRT-18, which were compared with phosphoproteins obtained by biosynthetic labelling. Accordingly, it was deduced that the enzymes are involved in phosphorylation of intracellular proteins, which lead to cell proliferation somehow and represent a component of the signal transduction pathway of growth promoting signals, and in addition, two enzymes as a signal transducer are surprisingly overlapping in physiologic activities and most probably closely related in sequence in the pathway. The data obtained are as follows. 1. Each of S6 kinase and PKC was purified 63 and 120 fold with a specific activity of 1790 and 4850 pmol of γ-phosphate of ATP transferred per min per mg of protein, respectively. 2. Both of S6 kinase and PKC were eluted at 0.07M NaCl concentration in 20mM Tris buffer pH7.5 on DEAE-Sephacel chromatography. 3. Molecular weights of PKC and S6 kinase were turned out to be 55,000 both. 4. As for the biosynthetic labelling of HRT-18 with [³²P]H₃PO₄, whole cell homogenate unravel 43KD, 36KD, 30KD and 20KD phosphoproteins, the activity of phosphorylation being highest in EGF stimulated cells. In the meantime, 40KD, 36KD and 22KD phosphorylation products were synthesized in mouse enibryo fibroblast, the generation of which was highest in the presence of PMA. 5. In the nucleus of HRT-18, elevated were the formation of 43KD, 36KD and 30KD phosphoproteins after biosynthetic labelling of HRT-18 with [³²P]H₃PO₄, wherein influences of growth factor, PMA or insulin conferred no influence on the profile. 6. Observation of in vivo substrates of S6 kinase and PKC are outlined as below. Two enzymes possess in common as their in vivo substates 43KD, 36KD, 20KD and 17KD proteins in the nucleus, 36KD, 20KD and 17KD proteins in the mitochondria, 67KD protein in the cytosol and 67KD, 36KD, 30KD, 30KD and 17KD proteins in the ribosome. Interestingly, 20KD and 17KD proteins existed in nucleus, mitochondria and ribosome in contrast to their absence in cytosol, which implies their possibility to be ribosomal proteins, where S6 kinase phosphorylated 17KD protein more strongly than PKC. On the other hand, phosphorylation of 30KD protein was discriminated only in ribosomal fractions, being more prominent also by S6 kinase than PKC. 7. The relatively consistent ratio of PKC to S6 kinase activity at each step of purification and the same NaCl concentration a t which two enzymes coeluted on DEAE-Sephacel chromatography and the similarities in physiologic substrates of two enzymes in subcellular fractions including nucleus, mitochondria, cytosol and ribosome, strongly suggest the intimate correlation of two enzymes in the signal transduction pathway of growth promoting signals, which can even lead to the assumption of their physically associated entity. 8. Discrepancy between the phosphoprotein profile generated by biosynthetic labelling with 32Pi and enzyme reaction with [γ-³²P]ATP reflect that the in vivo phosphate donor of S6 kinase and PKC may be a compartment separate from of the total cellular ATP.