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      • 液體種菌 接種에 의한 표고톱밥裁培 效果

        이태수,민두식,조남석 충북대학교 농업과학기술연구소 1998 農業科學硏究 Vol.15 No.-

        For cultivation on sawdust-bed of oak-mushroom until present time, inoculation of spawn on sawdust bed has been performed by sawdust spawn. But, liquid spawn may have advantages for rapid mass production of spawn, and now, sawdust-cultivation by liquid spawn inoculation should be applied instead of sawdust spawn. Therefore, investigations were performed to evaluate the effect of sawdust-cultivation by liquid spawn inoculation. The results were as follows : 1.When 11 kinds of liquid media were applied, the oak-mushroom culture medium was the most excellent in growth. Most suitable temperature at PDA was 25℃, and 22.5-27.5℃ in range were optimal for liquid culture. In liquid culture, amount of mycelial growth increases rapidly up to 40 days of cultivation. Incubation at fermentor brought yield of 106㎎ dry mycelia per 40㎖ media after 17 days. 2.In 1ℓ -spawn bottle, growth of mycelium by inoculation of 20㎖-liquid spawns were faster than 6g-sawdust spawn in spread of mycelia. On 2kg-bag culture, inoculations of 10㎖-, 20㎖- and 30㎖-liquid spawns were all slower than 20g-sawdust spawn in mycelial spread. So, amount increasement in ampunt of liquid spawn should be discussed. Yields of mushrooms until third sproutings of 2kg-bag culture were 580g in 30㎖-liquid spawn inoculation, but 510g, 486g and 470g from 20g-sawdust spawn, 20㎖-liquid spawn and 10㎖-liquid spawn, respectively. Thus, 30㎖-liquid spawn inoculation was highest in yield. Key words : oak-mushroom, sawdust-bed, liquid spawn.

      • SCOPUSKCI등재

        액체종균 접종에 의한 표고톱밥재배 효과

        조남석,민두식,이태수 한국목재공학회 1998 목재공학 Vol.26 No.1

        For cultivation on sawdust-bed of oak-mushroom until present time, inoculation of spawn on sawdust bed has been performed by sawdust spawn. But, liquid spawn may have advantages for rapid mass production of spawn, and now, sawdust-cultivation by liquid spawn inoculation should be applied instead of sawdust spawn. Therefore, investigations were performed to evaluate the effect of sawdust-cultivation by liquid spawn inoculation. The results were as follows : 1. When 11 kinds of liquid media were applied, the oak-mushroom culture medium was the most excellent in growth. Most suitable temperature at PDA was 25℃, and 22.5∼27.5℃ in range were optimal for liquid culture. In liquid culture, amount of mycelial growth increases rapidly up to 40 days of cultivation. Incubation at fermentor brought yield of 106㎎ dry mycelia per 40㎖ media after 17 days. 2. In 1ℓ-spawn bottle, growth of mycelium by inoculation of 20㎖-liquid spawns were faster than 6gsawdust spawn in spread of mycelia. On 2㎏-bag culture, inoculations of 10㎖-, 20㎖- and 30㎖-liquid spawns were all slower than 20g-sawdust spawn in mycelial spread. So, amount increasement in amount of liquid spawn should be discussed. Yields of mushrooms until third sproutings of 2㎏-bag culture were 5808 in 30㎖-liquid spawn inoculation, but 510g, 486g and 470g from 20g-sawdust spawn, 20㎖-liquid spawn and 10㎖-liquid spawn, respectively. Thus, 30㎖-liquid spawn inoculation was highest in yield.

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