토끼 심방근 및 혈관 평활근에서의 $Na^{+}/Ca^{2+}$ 교환기전에 관한 연구

김희주,문형로,엄융의,호원경,Kim, Hee-Ju,Moon, Hyung-Ro,Earm, Yung-E,Ho, Won-Kyung 대한생리학회 1988 대한생리학회지 Vol.22 No.1

In order to elucidate the regulatory mechanism of intracellular calcium ion concentrations, contractions or contractures induced by $Na^{+}-removal$, calcium-application or ouabain-treatment as an index of $Na^+/Ca^{2+}$ exchange activity were studied in atrial muscle or vascular smooth muscle (aorta and renal artery) of the rabbit. The magnitude of low sodium contractures in atrial trabeculae increased with sigmoid shape when external sodium concentrations were reduced to sodium-free condition, whereas that of calcium contracture intensified in a parabolic pattern when external calcium concentrations were elevated to 8 mM. $Na^{+}-removal$ contractures were induced in a duration-dependent manner to $K^{+}-free$ exposure and same findings were observed with ouabain treatment. $Na^{+}-free$ contractures were not affected by verapamil treatment, but stimulated by $100{\mu}M\;Mn^{2+}$ and inhibited by high concentrations of $Mn^{2+}\;(2{\sim}8mM)$ in a dose-dependent manner. Ryanodine which is known to suppress the release of calcium from internal store abolished spontaneous twitch contractions induced by $K^{+}-free$ solution, but had no effect on the development $Na^{+}-free$ contractures. Na-free contractures were not always induced in vascular smooth muscle preparations. Contractures by $O\;mM\;Na^+$ were usually seen in aorta, but not often in renal artery.$50\;mM\;K^+$, noradrenaline (NA) and angiotensin II (AII) always evoked very large contraction in all preparations of vascular smooth muscle. Contractures developed by $O\;mM\;Na^+$ were not sensitive to verapamil treatment as in atrial trabeculae, but were abolished by $100{\mu}M\;Mn^{2+}$. In contrast to $Na^{+}-free$ contractures, $Mn^{2+}(100{\mu}M)$ had no effect on the contractures induced by NA or 50 mM$K^+$. Caffeine in the concentration of 10 mM evoked transient contracture in the distal renal artery. The rate of spontaneous relaxation in caffeine contracture was dependent upon the concentrations of external sodium, and had double component of relaxation when the rate of relaxation was plotted in the semilogarithmic scale of relative tension versus time. Especially late components of relaxation had more direct relation to $Na^+$ concentrations. It could be concluded that $Na^+/Ca^{2+}$ exchange mechanism in the heart has a large capacity, inhibited by $Mn^{2+}$ but not by verapamil and ryanodine, while $Na^+/Ca^{2+}$ exchange system in vascular smooth muscle has a very low capacity especially in small artery, inhibited by low concentration of $Mn^{2+}\;(100{\mu}M)$ but not affected by verapamil and ryanodine.

토끼 심방근 및 혈관 평활근에서의 Na⁺/Ca²⁺ 교환기전에 관한 연구

김희주(Kim, Hee-Ju),문형로(Moon, Hyung-Ro),엄융의(Earm, Yung-E),호원경(Ho, Won-Kyung) 대한생리학회 1988 대한생리학회지 Vol.22 No.1

In order to elucidate the regulatory mechanism of intracellular calcium ion concentrations, contractions or contractures induced by Na⁺-removal, calcium-application or ouabain-treatment as an index of Na⁺/Ca²⁺ exchange activity were studied in atrial muscle or vascular smooth muscle (aorta and renal artery) of the rabbit. The magnitude of low sodium contractures in atrial trabeculae increased with sigmoid shape when external sodium concentrations were reduced to sodium-free condition, whereas that of calcium contracture intensified in a parabolic pattern when external calcium concentrations were elevated to 8 mM. Na⁺-removal contractures were induced in a duration-dependent manner to K⁺-free exposure and same findings were observed with ouabain treatment. Na⁺-free contractures were not affected by verapamil treatment, but stimulated by 100μM Mn²⁺ and inhibited by high concentrations of Mn²⁺ (2 ~ 8mM) in a dose-dependent manner. Ryanodine which is known to suppress the release of calcium from internal store abolished spontaneous twitch contractions induced by K⁺-free solution, but had no effect on the development Na⁺-free contractures. Na-free contractures were not always induced in vascular smooth muscle preparations. Contractures by O mM Na⁺ were usually seen in aorta, but not often in renal artery.50 mM K⁺, noradrenaline (NA) and angiotensin II (AII) always evoked very large contraction in all preparations of vascular smooth muscle. Contractures developed by O mM Na⁺ were not sensitive to verapamil treatment as in atrial trabeculae, but were abolished by 100μM Mn²⁺. In contrast to Na⁺-free contractures, Mn²⁺(100μM) had no effect on the contractures induced by NA or 50 mMK⁺. Caffeine in the concentration of 10 mM evoked transient contracture in the distal renal artery. The rate of spontaneous relaxation in caffeine contracture was dependent upon the concentrations of external sodium, and had double component of relaxation when the rate of relaxation was plotted in the semilogarithmic scale of relative tension versus time. Especially late components of relaxation had more direct relation to Na⁺ concentrations. It could be concluded that Na⁺/Ca²⁺ exchange mechanism in the heart has a large capacity, inhibited by Mn²⁺ but not by verapamil and ryanodine, while Na⁺/Ca²⁺ exchange system in vascular smooth muscle has a very low capacity especially in small artery, inhibited by low concentration of Mn²⁺ (100μM) but not affected by verapamil and ryanodine.

토끼 동방결절 부위에 따른 Na-Pump활동도의 차이에 관한 연구

서종진(Seo, Jong-Jin),문형로(Moon, Hyung-Ro),엄융의(Earm, Yung-E) 대한생리학회 1985 대한생리학회지 Vol.19 No.2

Electrophysiological difference of the central and peripheral area of the sinoatrial node in the rabbit was studied by glass microelectrode technique. Effects of K⁺, NaK⁺, CsK⁺, adrenaline and ouabain on the action potential of the two areas were investigated, and transient hyperpolarization (K⁺-induced hyperpolarization) which developed following readmission of potassium after having pre-treated with K⁺-free Tyrode solution for 10 minutes was analyzed. The results obtained were as follows ; 1) The frequency of the spontaneous action potential recorded in the periphery of the SA node was faster than the central area. Reduction by Cs⁺ and increase by O mM K⁺, 10^-6 M adrenaline and 10^-6M ouabain in the frequency of action potential were noticed more prominently in the peripheral than the central area. On the contrary, the frequency in the central area was more decreased than the Peripheral area by 13 mH K⁺ and 1 mM Co²⁺. 2) The amplitude of the K+_induced hyperpolarization was very small in the central area but large in the peripheral area. Transient hyperpolarization was abolished by ouabain and low sodium, and decreased by cooling the tissue (17℃). 3) By changing the concentration of Ca²⁺ in the perfusate, the amplitude and the rate of transient hyperpolarization were increased in the high Ca²⁺ concentration. It could be concluded that the central area of the SA node is less susceptible to the inhibition of Na-Pump and more susceptible to Ca-blocker and high concentration of K⁺. The Na-Pump activity of the central area measured by means of transient hyperpolarization is found to be much less active than that of the peripheral area.

톡소플라스마 곤디의 세포내 배양에 있어서 세포 주에 따른 감수성 비교

박병규(Byung-Kiu Park),문형로(Hyung Ro Moon),유재란(Jae-Ran Yu),국진아(Jina Kook),채종일(Jong-Yil Chai),이순형(Soon-Hyung Lee) 대한기생충학열대의학회 1993 The Korean Journal of Parasitology Vol.31 No.3

선천성 첨내반족을 동반한 Pfeiffer증후군

이덕용(Duk Yong Lee),이계형(Kye Hyoung Lee),김한수(Han Soo Kim),문형로(Hyung Ro Moon) 대한정형외과학회 1989 대한정형외과학회지 Vol.24 No.6

Cornelia de Lange 증후군에 동반된 Legg-Perthes씨 병의 치험

이덕용(Duk Yong Lee),최인호(In Ho Choi),문형로(Hyung Ro Moon),윤강섭(Kang-Sup Yoon),이명철(Myung Chul Lee),안재훈(Jas Hoon Ahn) 대한정형외과학회 1990 대한정형외과학회지 Vol.25 No.2

Immunoblot analysis for serum antibodies to Pneumocystis carinii by age and intensity of infection in rats

Sung-Tae HONG(홍태성),Mejeong LEE(이미정),Min SEO(서민),Dong Hyun CHOO(주동현),Hyung Ro MOON(문형로),Soon-Hyung LEE(이순형) 대한기생충학열대의학회 1995 The Korean Journal of Parasitology Vol.33 No.3

소아에서의 두개인두관종 : 임상 및 내분비학적 고찰

문형로,양세원 대한내분비학회 1989 Endocrinology and metabolism Vol.4 No.3

We observed the clinical characteristics including the symptoms and signs, and radiologic findings in 14 children with craniopharyngioma. Their endocrinolgical characteristics including growth and pituitary function of pre- and post- treatment were also evalusted. The results of above evaluations were as belows; 1) Most clinical symptoms they complaint were those from increased intracranial pressure and visual symptoms. But the endocrine symptoms and signs such as polyuria, short stature compared with the normal children of same chronological age, or obesity could be observed (Table 1). 2) Bone ages were retarded in 12 children (86%). Calcifications in the tumor mass and enlarged sella turcica were retarded in all children. In all cases tumor was located in the suprasellar area and in 6 cases mass was located in the sellar area also. 3) The responses of insulin in children with craniopharyngioma to the oral glucose loading were significantly higher than that in normal children (p$lt;0.05) (Fig. 1). Growth velocity and weight gain after treatment were not correlated with the peak reponses of insulin (Fig. 2). The growth velocity during the first 6 months after treatment was not correlated with the weight gain during the same period (Fig. 3). The growth velocity during two years after treatment was higher significantly than that of before treatment (p$lt;0.05) (Fig. 4). 4) All of the patients had GH deficiencies and central hypothyroidism after treatment. Most of the central hypothyroidism came from the deficiency of TRH. All of the patients had also ADH deficiencies immediately after treatment but three children had got rid of ADH deficiency spontaneously during the follow up. Gonadotropin deficiency was also observed in all cases tested, whose bone ages were above 12 years old. ACTH was most resistant one among the pituitary hormones and its deficiency was partial mostly. In general, the incidence of the pituitary hormone deficiencies increased after treatment and it may come from the destruction of the surgical procedures and radiation therapy (Table 2). In summary, there may be the endocrinological symptoms and signs at initial presentations in the children with craniopharyngioma and the growth velocity and other endocrinological manifestations such as polyuria should be monitored closely. All children with craniopharyngioma should have pituitary eveluation before and after treatment because they have more than one pituitary hormone deficiency and they should be managed early with hormone to prevent the long-term morbidity after treatment. (J. Kor Soc Endocrinol 4:209~217, 1989)

소아에서의 진성 성조숙증

문형로,조병규,양세원,황용승 대한내분비학회 1988 Endocrinology and metabolism Vol.3 No.2

22 patients who were diagnosed as true precocious puberty were reviewed through the hospital records retrospectively. They were diagnosed by the clinical manifestations (including secondary sexual characteristics, height Velocity and gelastic seizure), bone age, abdominal ultrasonopragphy and brain CT. The final results were as follows; 1) The ratio of male to female was 6:16 and mean age at first presentation was 5.7±4.4 years in male and 4.4±2.3 years in female. 2) 9 patients had the first clinical signs of precocity within the first year of age, of whom 5 patients had a brain tumor (4 patients, hypothalamic hamartoma and 1 glioma)(Fig. 1). 3) The causes of the true precocity were presented in table 2 and the clinical manifestations were presented in table 3. In 12 patients, their height were above 97 percentile for their chronological age at initial presentation and kept above 97 percentile throughout the period of follow-up. The bone ages of all cases were far advanced for their chronological age (p$lt;0.05)(g. 3). 4) The final adult heights which were predicted by the Bayley-Pinneau method were measured in our patients whose bone ages were above 7 years. The predicted final adult heights in 12 female patients were from 135.1 cm to 159.5 cm(Table 1). 5) Serum concentration of LH was higher significantly in the group of brain tumor than that of idiopathic, while serum concentration of FSH was not different in the former from that in the latter. 6) The improvement of signs of secondary sexual characteristics was observed, but improvement of the height velocity and advancement of bone age could not be observed with medroxyprogesterone (MPA). In conclusion, we recommend the abdominal ultrasonography to differnetiate between the true precocity and pseudoprecocity in patients who present the secondary sex characteristics prematurely, and brain CT to search for the brain lesions, such as tumor, in patients with the true precocious puberty, especially who has the gelastic seizure. To control the sexual characteristics and excessive growth velocity, long-acting LHRH analogues should be available in Korea.

胎兒를 위한 妊娠中의 注意

文炯魯 대한의학협회 1966 대한의학협회지 Vol.9 No.5