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액체크로마토그래프-탠덤질량분석기(LC-MS/MS)를 이용한 소변 내 D-, L- Lactate 분리 및 정량
문철진,양송현,Moon, Chul Jin,Yang, Song Hyun 대한유전성대사질환학회 2015 대한유전성대사질환학회지 Vol.15 No.2
사람의 신체내에서 주로 존재하는 lactate는 L-lactate로서 몇몇 선천성대사이상과 관련하여 증가된다. 최근 2형 당뇨병(type 2 diabetes)과 만성적인 염증성 위장질환(inflammatory bowel disease)과 관련하여 증가되는 D-lactate를 L-lactate와 분리해야 하는 요구도가 높아졌다. 이에 액체크로마토그래프-탠덤질량 분석기를 사용하여 소변 내 D-, L-lactate를 분리하는 방법을 확립하였으며, 분석시간과 정밀성, 정확성, 특이성 등에서 신뢰성 있는 방법임을 확인하였다. Purpose: Lactate has two optical isomers, L-lactate and D-lactate. In human L-lactate is the most abundant enantiomer of lactate. As plasma and urinary levels of L-lactate is associated with inherited metabolic disorders in general, D-lactate have been linked to the presence of diabetes and inflammatory bowel disease. Previously developed techniques have shown several limitations to further evaluate D-lactate as a biomarker for this condition. In this paper, we describe a highly sensitive, specific and fast liquid chromatography tandem mass spectrometry (LC-MS/MS) method for the analysis of D-, L-lactate in urine. Methods: D- and L-lactate were quantified using high performance liquid chromatography tandem mass spectrometry (LC-MS/MS) with labelled internal standard. Samples were derivatized with (+)-O,O'-diacety-L-tartaric anhydride (DATAN) and seperated on a Poroshell 120 EC-C18 column. Results: Quantitative analysis of D-, and L-lactate was achieved successfully. Calibration curves were linear (r>0.999) over $0.5-100{\mu}g/mL$. Stabilities for samples were within the 10% varation. Inter- and Intra-day assay variations were below 10%. Conclusion: The presented method proved to be suitable for the quantitation of D- and L-lactate and opens the possibility to explore the use of D-lactate as a biomarker.
이염화망간을 투여한 흰쥐의 조직중 망간과 Malondialdehyde간의 관계
문철진,이수진,이세훈 大韓産業醫學會 2000 대한직업환경의학회지 Vol.12 No.3
목 적 : 이 염화망간을 투여한 백쥐를 이용하여 망간이 유의하게 축적된 장기와, 장기내 망간과 MDA의 상관관계로서 세포내 지질과산화반응을 평가하였다. 방 법 : 생후 6주, 체중 200∼250 g의 수컷 횐쥐(Sprague-Dawley) 12마리에게 이염화망간(MgCl2)을 4 mg/kg 농도로 한달간 복강내 주사하고, 동일 조건의 횐쥐 9마리에게 생리식염수를 1ml씩 한달간 복강내 주사하였다. 혈액, 뇌, 간, 비장, 췌장, 콩팥을 채취한 뒤, 각 조직중의 망간농도와 malondialdehyde를 정 량하였다. 결 과 : 망간의 농도가 대조군에 비해 유의하게 높았던 조직은 혈장, 뇌, 췌장 및 간장이 였다. 대조군에 비해 실험군에서 조직중 malondialdehyde 농도가 유의하게 높았던 조직은 혈액, 뇌, 췌장이 였다. 대조군에 비해 망간농도와 MBA농도가 모두 유의하게 높았던 혈장, 뇌, 췌장중의 두 변수간에는 유의한 상관관계가 있었다. 결 론 : 이상의 결과 고농도의 망간을 투여한 경우 뇌, 췌장에 특이하게 망간이 축적되며, 이들 장기에서 지질과산화반응이 촉진되어 그 최종 대사산물인 MDA의 농도가 증가한다는 결론을 얻었다. Objectives : This research was intended to investigate the relationship between manganese and malodndialdehyde concentration in tissues of rats exposed to maganele chloride. Methods : The study groups were 12 manganese treated rats and 9 control rats. Manganese treated rats were given intraperitoneally manganese chloride (Mn2+, 4 mg/kg) daily for a period of 30 days except Sunday. Control rats were i운ected 1ml of saline. The plasma manganese concentrations of rats were determined by graphite furnace atomic absorption spectrometry. The tissue manganese concentration was determined by flame atomic absorption spectrometry. Malondialdehyde, the product of lipid peroxidation was determined by ultraviolet-visible spectrophotometry. The plasma malondialdehyde was determined by gas chromatography with mass-detector. Protein concentration was quantified by ultraviolet-visible spectrometry and was used for the compensation of tissue malondialdehyde and manganese concentration. Results : Manganese concentrations of plasma, brawn, liver, and pancreas were very significantly higher in the manganese-treated rats than in the control rats. Malondialdehyde concentration of plasma, brain, and pacrease were significantly higher In the manganese-treated rats than in the control rats. The concentration of malondialdehyde was correlated with manganese levels in plasma, brain and pancreas. Conclusion : Based on the results obtained as above, it was concluded that the matrondialdehyde, product of lipid peroxidation was related to the cell death due to dosing excess manganese.
익형에 발생하는 부분 캐비테이션 후연영역 유동장 특성 연구
문철진,김시영 한국마린엔지니어링학회 1988 한국마린엔지니어링학회지 Vol.12 No.1
Most of the recent for the flow field hydrofoil in partially cavitating condition are the ones which are linearized, and the problem of cavity end region for hydrofoil is not verified. This paper deals with a study on characteristics of end region flow field for partially cavitating hydrofoil by using a characteristics of shear turbulence flow and nonlinear cavity flow theory. The results obtained as follows : 1) Shear layer thickness is decreased gradually going to the end section of hydrofoil. When attack angle is large, it is appeared largely at the region of partial cavitation after its collapsing. 2) The fluctuation velocity of a second-degree relative direction have minimum value at the front of hydrofoil or at the end of hydrofoil. The difference for the validity of attack angle is appeared largely at the surrounding of .chi.$_{e}$ point. 3) The fluctuation velocity of transverse direction decrease from the maximum thickness of cavitation to the end of hydrofoil, but it undergoes largely the effect of pressure recovery. The difference is larger at the region of partial cavitation after its collapsing than at the of hydrofoil. 4) The distribution of Reynolds stress have maximum values at the region of partial cavitation after its collapsing and the end of foil, and the larger attack angle, the larger the distribution of value.e.
외부 수압을 받는 필라멘트 와인딩 후판 복합재 원통의 좌굴 연구
문철진(Chul-jin Moon),허성화(Seong-Hwa Hur),안정희(Jung-Hee Ahn),권진회(Jin-Hwe Kweon),최진호(Jin-Ho Choi),조종래(Jong-Rae Cho),조상래(Sang-Rae Cho) 한국항공우주학회 2009 韓國航空宇宙學會誌 Vol.37 No.2
외부 수압을 받는 필라멘트 와인딩 후판 복합재 원통의 좌굴 및 파손을 유한요소법과 시험으로 연구하였다. 자체 개발 프로그램인 ACOS와 상업용 프로그램인 M5C.NASTRAN(선형)과 MSC.MARC(비선형)를 이용한 유한요소해석을 수행하였다. 복합재 원통 시편은 T700 카본-에폭시로 필라멘르 와인딩 기법에 의해 [±30/90]<SUB>FW</SUB>, [±45/90]<SUB>FW</SUB>, [±60]<SUB>FW</SUB>, [±60/90]<SUB>FW</SUB>의 각도로 제작하여 수압시험을 수행하였다. 사용한 세 가지 프로그램 중 ACOS가 시험값과 1.7~14.3%의 차이를 보이며 좌굴 압력을 가장 잘 예측하였다. 모든 경우에 좌굴 후 지지하중이 초기 좌굴하중보다 커지지 못하고 최종파손으로 연결되었다. The buckling and failure of filament wound thick composite cylinders under external hydrostatic pressure were investigated by the finite element analysis and test. ACOS, MSC.NASTRAN, and MSC.MARC were used for finite element analysis. T700 carbon-epoxy filament wound composite cylinders were fabricated to have winding angles of [±30/90]<SUB>FW</SUB>, [±45/90]<SUB>FW</SUB>, [±60]<SUB>FW</SUB>, and [±60/90]<SUB>FW</SUB>, and tested to verify the finite element analysis. Among the softwares, ACOS predicted buckling load the best with about 1.7~14.3% deviation from test. Analysis and test shows cylinders do not recover the initial buckling pressure after buckling and directly lead to final failure.