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문주훈,박주철,고기종 朝鮮大學校 口腔生物學硏究所 1998 口腔生物學硏究 Vol.22 No.1
The purpose of this study was to investigate the cytotoxocity of dental restorative materials - dental amalgam, type Ⅱ casting gold alloy, composite resin inlay - on fibroblasts cultivated from human gingiva. Viable cell count and succinate dehydrogenase activity test were preformed to evaluate the short-term cytotoxicity. According to 2,4 and 6 days vital cell count and succinate dehydrogenage activity test to fibroblast were evaluated. The results were as follows : 1. In the cell morphology observation, viable cell number of amalgam & composite resin inlay group decreased after 4 days. 2. In the test of viable cell count, amalgam & composite resin inlay group showed cytotoxicity compared to the control & type Ⅱ casting gold group after 2 & 4 days, ranks ordered the relative cytotoxicity of the tested materials, as follows : amalgam, composite resin inlay, type Ⅱ casting gold group. 3. Amalgam and composite resin inlay group marked cytotoxicity during 2 days, but the cytotoxicity was reduced after 6 days. Type Ⅱ casting gold group showed the least cytotoxicity among the three experimental materials. 4. In the test of SDH activity by millipore filter method, amalgam and composite resin inlay group showed mild cytotoxicity.
흰쥐에서 치아손상 후 삼차신경절에서 vasoactive intestinal polypeptide 면역반응세포에 관한 연구
김흥중,박주철,김두현,문주훈 朝鮮大學校 口腔生物學硏究所 1999 Oral Biology Research (Oral Biol Res) Vol.23 No.2
The effect of tooth injury on the expression of vasoactive intestinal polypeptide(VIP) in trigeminal ganglion was examined immunohistochemically in rat. The animals were divided into normal and 2 weeks experimental groups. The trigerminal granglion was removed and immersed in the 4% paraformaldehyde in 0.1M phosphate buffer. Serial forzon sections were gained with a cryostat. The rabbit anti-VIP was used as primary antibody and fluorescene isothiocynate(FITC)- conjugated anti-rabbit IgG as secondary antibody in immunofluorescence staining. The stained slides were observed under confocal laser scanning microscope. The results were as follows; 1. The positive ratio of VIP immunoreactive neurons; The positive ratio of VIP immunoreactive neurons in the mandibular part of trigeminal ganglion was 6.52±2.31% in normal group and 24.73±7.27 in 2 weeks after tooth injury group. 2. The relative fluorescence intensity of VIP immunoreactive neurons; The relative fluorescence intensity of VIP immunoreactive neurons in the mandibular part of trigeminal ganglion was 96.92±14.95 in normal group and 165.37±25.73 in 2 weeks after tooth injury group. The relative fluorescence intensity of w weeks experimental group was increased 68.5,% than intensity of normal group. 3. These results indicate that immunoreactivity and fluorescence intensity of VIP immunoreactive neurons were increased in trigeminal ganglion following tooth injury.