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연구논문 : 단감(Diospyros Kaki L.)껍질 추출물의 신경세포 보호 효과
이미라 ( Mi Ra Lee ),문성희 ( Seong Hee Moon ),최애란 ( Ae Ran Choi ),이승철 ( Seung Cheol Lee ),안광환 ( Kwnag Hwan Ahn ),박해룡 ( Hae Ryong Park ) 한국식품조리과학회(구 한국조리과학회) 2011 한국식품조리과학회지 Vol.27 No.4
This study was performed to assess the neuroprotective effects of methanolic extracts from sweet persimmon peel (PPE) against glutamate-induced cytotoxicity in hybridoma N18-RE-105 cells. The neuroprotective effects of PPE in N18-RE-105 cells were measured using the MTT reduction assay, LDH release assay, and phase-contrast microscopy. The results of the MTT reduction assay showed that treating cells with 500 μg/mL PPE resulted in cell viability of 66.9%. Additionally, the morphological changes and the results of the LDH release assay showed that glutamate-induced damage to nerve cells was strongly inhibited by PPE. GSH content of N18-RE-105 cells was 3.5 μM compared to that of the control, whereas pretreatment with 500 μg/mL PPE increased GSH content by 4.7 μM. PPE was fractionated with hexane, and that layer had the highest neuroprotective effects in glutamate-stressed N18-RE-105 cells. In conclusion, our data showed that glutamate potentiated the effects of N18-RE-105 cell death by a mechanism involving oxidative stress. Therefore, PPE may be a potential candidate for prevention and therapy of neurodegenerative diseases.
Hydrogen peroxide가 유도하는 세포독성으로부터 PC12 세포를 보호하는 하고초(Prunella spica) 추출물의 영향
Hyun-Jung Kim(김현정),Jeung-Min Lee(이정민),Seong-Hee Moon(문성희),Hae-Ryong Park(박해룡) 한국생명과학회 2010 생명과학회지 Vol.20 No.7
활성산소에 의해 유도되는 산화적 스트레스가 노화와 관련된 각종 퇴행성 신경질환의 원인으로 밝혀짐에 따라 본 연구에서는 활성산소종인 H₂O₂가 유도하는 강력한 산화적 스트레스로부터 신경세포를 보호하는 물질을 탐색하기 위하여 하고초 추출물(PSE)에 관하여 연구하였다. PC12 세포를 이용하여 MTT reduction assay, LDH release assay 및 colony formation assay 등 여러 가지 생물학적인 assay를 통하여 PSE의 세포 보호효과를 확인하였다. 그리고 광학 현미경을 이용한 형태학적 변화 관찰에서도 H₂O₂ 처리군에 비해 높은 세포 보호효과를 확인할 수 있었으며, Hoechst 33342 염색과 세포주기 분석을 통하여 PSE의 높은 apoptosis 억제효과를 확인할 수 있었다. 이상의 결과로부터 하고초는 H2O2에 의해 유도된 세포독성으로부터 PC12 세포손상을 강력하게 억제하는 효과가 있다는 것을 확인할 수 있었으며, 퇴행성 신경질환에 대한 새로운 치료제로서의 가능성을 제시하였다. The oxidative stress induced by reactive oxygen species (ROS) may play an important role in the pathogenesis of neurodegenerative diseases. In this study, we investigated the neuroprotective effects of methanolic extracts of Prunella Spica (PSE) against H₂O₂-induced oxidative stress in PC12 cells. The cells exposed to H₂O₂-induced oxidative stress were treated with various concentrations of PSE; this treatment resulted in the induction of a dose-dependent protective effect, which was evidenced by the results of MTT reduction assay, lactate dehydrogenase (LDH) release assay, morphological assay, and colony-formation assay. Interestingly, we also observed reduction of apoptotic bodies in the Hoechst staining and flow cytometric analysis. These data show that apoptosis was significantly suppressed in the PC12 cells that were exposed to H₂O₂-induced oxidative stress and treated with PSE. These results suggest that Prunella Spica could be a new potential protective agent against H₂O₂-induced oxidative stress.