표피종양의 Involucrin 표현에 관한 연구

문두찬(Doo Chan Moon),현명기(Myung Ki Hyun),권경술(Kyung Sool Kwon),정태안(Tae Ahn Chung) 대한피부과학회 1989 대한피부과학회지 Vol.27 No.2

Involucrin is a recently recognized structural component of mature squamous epithelial cells and is considered as a marker of normal eratinocyte differentiation and ma.turation. In this study peroxidase-antiperoxidase techniques were used to assess involucrin expression in histologic sections of normal skin and a variety of epidennal tumors including squamous cell carcinomas(25 cases), keratoacanthomas(11 cases), basal cell carcinomas(20 cases), trichoepitheliomas(5 cases), Howens diseases(12 cases), arsenic keratoses(10 cases), actinic keratoses(10 cases) and Pagets diseases(2 cases). The results were as follows : l. In normal skin, the upper third of the viable epidermis showed diffuse cytoplasmic staining for involucrin. In hair follicles, the lower area of inner root sheath and inner area of the outer root sheath stained positively. The sebareous glands did not stain, but the ducts of sebaceous glands were positive. 2. Keratoacanthomas showed a relatively homogeneous staining pattern for inirolucrin ', all cells except basal cells stained with mild to moderate intensity. In contrast, squamous cell carcinomas disclosed a highly irregular involucrin staining pattern with marked variation in staining intensity from cell to cell. 3. Basal cell carcinomas were negative for involucrin except squamous horn cysts, and the epidermis overlying basal cell carcinomas showed the field effect, that is, the epidermis overlying the tumors exhibited diffuse, homogeneous positive staining of cells in all layers of the epidermis. 4. ln trichoepitheliomas, the involucrin reactivity was negative as in basal cell carcinoma.s, but the field effect was not observed. 5. In Elowens disease, actinic keratosis, arsenic keratosis and Pagets disease, the positivit.y for involucrin staining extended deeper into the stratum malpighii than was observed wit,h normal epidermis. And areas showing irregular patchy pattern of involucrin staining were considered to have the potentiality of malignant invasive change much more than the negative or homogeneous areas. Paget's cells were negative for involucrin as the adjoining keratinocytes in the lower portion af epidermis.

융합성 망상 유두종증의 임상적 및 병리조직학적 관찰

장호선,문두찬,권경술,정태안 ( Ho Sun Jang,Doo Chan Moon,Kyung Sool Kwon,Tae Ahn Chung ) 대한피부과학회 1991 대한피부과학회지 Vol.29 No.4

N/A Clinical and Histopathological Observation of Confluent and Reticulated Papillomatosis Ho Sun Jang, M.D., Doo Chan Moon, M.D., Kyung Sool Kwon, M.D., Tae Ahn Chung, M.D. Department of Dermatology, College of Medicine, Pusan National University, Pusan, Korea Confluent and reticulated papillomatosis is a rare but clinically distinct dermatosis of which the etiology is unknown. We observed the clinical and histopathological findings of 14 patients of confluent and reticulated papillomatosis who had visited Pusan National University Hospital, and studied mycologically Pityrosporum orbiculare. The results were as follows : 1. The mean age of onset was 17.7 Years and the most common site of initial involvement was epigastrium(57.1%), and the most common sites of involvement were abdomen(85.7%), and back(85.7%). 2. Positive KOH/Parker ink direct smears were observed in 9 cases, and they were treated with oral and topical antifungal agents. Six out of the patients were responsive to these treatment. 3. Skin biopsy was performed in 13 patients. The major histologic features were hyperkeratosis, focal acanthosis, papillomatosis, parakeratosis, slight atrophy of stratum malpighiii, decreased granualr cell layer, hypermelanosis of basal cell layer, and slight perivascular infiltrate of mononuclear cells. Periodic acid-Schiff stain showed Pityrosporum orbiculare yeasts in the stratum corneum in 7 cases. 4. The cultrued Pityrosporum orbiculare colonies were inoculated on the inner ear surface of 5 rabbits, and one week after inoculation, thick yellowish scaly crusts were seen in 3 of 5 rabbits. Therefore, we believe that Pityrosporum orbiculare may play an important role in the pathogenesis of confluent and reticulated papillomatosis. (Kor J Dermatol 29(4) : 471-480, 1991)

경화 위축성 태선과 경피증의 감별진단에 있어서 렉틴의 의의

이준호,문두찬,권경술,정태안 ( Joon Ho Lee,Doo Chan Moon,Kyung Sool Kwon,Tae Ahn Chung ) 대한피부과학회 1993 大韓皮膚科學會誌 Vol.31 No.3

Background : Differential diagnosis of lichen sclerosus et atrophicus( SA) and scleroderma is occasionally difficult. Objective : The purpose of this study was to attempt differentiation between the two diseases using imrnunohistochemical stain and lectins. Methods : Paraffin-embeddred sections of 4 cases of LSA and 11 cases of scleroderma were evaluated for this study. Using lectins, such as peanut agglutinin(PNA), siybean agglutinin(SBA), Ulex europaeus agglutinin-I(UEA-I) and Dolichos biflorus agglutinin(DBA) and the avidin-biotin-peroxi-dase complex(ABC) technique, differential lectin binding patterns betv een the two diseases were examined. Results : In the case of LSA, PNA and SBA stained the upper and lower spinous layer of the epidermis, and UEA I also stained the spinous layer of the epidermis weakly, but no DBA was stained. In the case of scleroderma, PNA stained not only the spinous layer but also the basal layer, SBA stained the upper half of the spinous layer but not the lower half of the pinous layer of epidermis. But UEA-I stained the vascular endothelial cells of dermis instead of epidermis, and DBA stained only the basal layer of epidermis. Conclusion : Staining of these 4 lectins on paraffin-embedded sectians using ABC teehnique could be helpful in differenting LSA and scleroderma. (Kor J Dermatol 1993; 31(3): 329-336)

인공피복재가 창상치유에 미치는 영향에 관한 실험적 연구

오창근,문두찬,권경술,정태안 ( Chang Keun Oh,Doo Chan Moon,Kyung Sool Kwon,Tae Ahn Chung ) 대한피부과학회 1992 대한피부과학회지 Vol.30 No.5

궤양성대장염이 동반된 급성 발열성 호중구성 피부병

장호선,이장수,문두찬,권경술,정태안 ( Ho Sun Jang,Jang Soo Lee,Doo Chan Moon,Kyung Sool Kwon,Tae Ahn Chung ) 대한피부과학회 1991 대한피부과학회지 Vol.29 No.4

A Case of Acute Febrile Neutrophilic Dermatosis Associated with Ulcerative Colitis Ho Sun Jang, M.D., Jang Soo Lee, M.D.*, Doo Chan Moon, M.D., Kyung Sool Kwon, M.D., Tae Ahn Chung, M.D. Department of Dermatology, College of Medicine, Pusan National University, Department of Dermatology, Pusan Medical Center*, Pusan, Korea We report a case of acute febrile neutrophilic dermatosis associated with ulcerative colitis occurred in a 58-year-old woman who showed well-demarcated, painful, erythematous papules, nodules, and plaques on the face, posterior neck, and both upper and lower extremities. The patient also experienced frequent diarrhea and intermittent bloody stool for about 10 years. Laboratory findings revealed polymorphonuclear leukocytosis, elevated erythrocyte sedimentation rate, and occult blood in stool. Findings of sigmoidoscopy, barium enema, and rectal biopsy were consistent with diagnosis of ulcerative colitis. Skin biopsy from the lesion on the posterior neck showed dermal edema resulting in subepidermal blisters and a dense inflammatory cell infiltration composed predominetly of polymorphonuclear leukocytes and a few mononuclear cells in the dermis. Biopsy from the lesion on the right shin revealed a moderate inflammatory cell infiltrate in the septa of the subcutaneous fat layer, composed chiefly of the lymphohistiocytes and a few eosinophils. The symptoms and signs responded well to orally administered prednisolone and sulfasalazine. This is a very rare case of acute febrile neutrophilic dermatosis with which ulcerative colitis was associated. (Kor J Dermatol 29(4) : 522-528, 1991)

백서에서의 문신육아종 발생에 관한 실험적 연구

김초록,오창근,문두찬,권경술,정태안 ( Cho Rok Kim,Chang Keun Oh,Doo Chan Moon,Kyung Sool Kwon,Tae Ahn Chung ) 대한피부과학회 1991 대한피부과학회지 Vol.29 No.6

N/A Decorative tattooing has become popular among Korean women, done by many beauticians offering this service. This has resulted in various serious complications including cutaneous granulomas. We tried to produce granulomatous reaction in rats following the injection of eyebrow tattoo pigment. Thiry rats were divided into 3 groups and were injected in tradermally with following materials:tattoo pigment, tattoo pigment and Freund complete adjuvant, and Freund complete adjuvant, respectively. Using an inductively coupled plasma(ICP) spectrometry, quantitative elemental analysis of the original sample of the tattoo dye was done. The results were as follows : 1. In tattoo pigment injected group, foreign body granulomas we`e observed in 7 of 10 rats at one month, which consisted of macrophages, mononuclear cells and foreign body giant cells around tattoo pigment. Little difference was seen in the granulomas between one and two months. In the remaining 3 rats, macrophages that had ingested tattoo pigment and mononuclear inflammatory cells were diffusely scattered in the dermis. 2. In the tattoo pigment and Freund complete adjuvant injected group, all 10 showed foreign body granulomas at one month. Little difference was seen in the granulomas between one and two months. 3. In Freund complete adjuvant injected control group, macrophages and mononuclear inflammatory cells were present in the dermis, but well formed granulomas were not present. 4. By ICP spectrometry, cobalt(11280ppm), calcium(1101ppm), chromium(238ppm), lead(441.6ppm), aluminum(56.52ppm), sodium(456.8ppm), potassium(457.6ppm), magnesium(142.28ppm), phosphorus(25.95ppm), nickel(11.85ppm), copper(1.37ppm) and titanium(0.34ppm) were detected from the tattoo pigment.

전신성 환상 육아종

박형근(Hyung Geun Park),문두찬(Doo Chan Moon),권경술(Kyung Sool Kwon),정태안(Tae Ahn Chung) 대한피부과학회 1989 대한피부과학회지 Vol.27 No.3

We experienced a case of generalized granuloma annular e in a 68-year-old man. Many factors have been implicated in the genesis of this disease, but in this case no etiologic factors were identified. He was treated successfully with systemic corticosteroid for about l year.

Etretinate ( Tigason R ) 투여로 호전된 가족성 양성 만성천포창

박형근(Hyung Geun Park),문두찬(Doo Chan Moon),권경술(Kyung Sool Kwon),정태안(Tae Ahn Chung) 대한피부과학회 1988 대한피부과학회지 Vol.26 No.5

We report a case of familial benign chroinic pemphigus in a 47 year old man. We had tried various treatments but no effect had been noted with systemic steroids, tetracycline, cephalosporin, rifampin, dapsone, and local application with antibiotic ar..d eteroid ointment. Thus the patient was started on 50mg of etretinate(Tigason) daily and almost complete resolution of the skin lesions was noted two moths after treatment. He was maintained on 15mg of etretinate daily and follow up evaluations for six months revealed no recurrence and no exacerbation of the lesions.

Ehlers - Danlos 증후군 - I형과 II 형의 혼합된 임상소견을 보인 -

박성민(Seong Min Park),문두찬(Doo Chan Moon),권경술(Kyung Sool Kwon),정태안(Tae Ahn Chung),김광만(Kwang Man Kim) 대한피부과학회 1988 대한피부과학회지 Vol.26 No.3

We presented a case of Ehlers-Danlos syndrome(ED syndrome) in s 21-year- old male, who had hyperextensibility of skin, skin fragility, and cigarette paperlike atrophic scars with no joint problems since infancy. There was a family history which was suggestive of autosomal dominant trait. Microscopic examination of the skin biopsy specimen from the normal appearing skin on the right forearm showed no abnormal appearance. In this case the manifestations of skin including marked hyperextensibility might be in accord with type I (gravis) ED syndrome, but the joint manifestation did not correspond to this type. It might be hard to classify this case according to the eleven types of ED syndrome. Wed rather consider this case as a. mixed form of type I and type Il ED syndrome.

Letterer - Siwe 병 3예

차형기(Hyung Gi Cha),문두찬(Doo Chan Moon),권경술(Kyung Soll Kwon),정태안(Tae Ahn Chung) 대한피부과학회 1994 대한피부과학회지 Vol.32 No.1

Letterer-Siwe disease is one of Langerhans cell histiocytosis, with Hand-Schuller-Christian disease and eosinophilic granuloma, characterized by proliferation of Langevha is cell. The clinical course of Letterer-Siwe disease is acute fulmunant of casionally fatal, involving skin and many other internal organs, such as the lymph node, liver, spleen, ung and bone. We present 3 cases of Letterer-Siwe disease with characteristic cutaneous findings and revealed Langerhans granules by imrriunohistochemical stain and electronmicrc scopic examination. All patients died in spite of combined chernotherapy. (Kor J Dermatol 1994; 32(1): 186-192)