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- 저자 : 남택정 ( Teak Jeong Nam ) (검색결과 3 건)
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사료의 단백질이 조피볼락 혈액중 Insulin - like growth factor - 1 ( IGF - 1 ) 및 IGF - binding protein - 3 에 미치는 영향
남택정(Teak Jeong Nam),권미진(Mi Jin Kwon),이상민(Sang Min Lee),박기영(Kie Young Park),김윤(Yun Kim),박승렬(Sung Real Park),변재형(Jae Hyeung Pyeun) 한국수산과학회 2001 한국수산과학회지 Vol.34 No.5
조피볼락을 실험어로 하여 사료 단백질의 함량과 단백질원을 달리하여 공급한 후 동물의 성장인자 중 하나인 Insulin-like growth factor-I (IGF-I)과 IGFBP-3의 혈중농도를 검토하였다. 사료 단백질의 함량이 50%∼60% 급여군에서 성장이 좋았고, 혈액중 IGF-I 농도도 높게 나타났다. 그리고 콘글루텐과 우모분보다 질적으로 우수한 어분, 대두박분, 육분을 첨가한 사료로 사육한 조피볼락의 혈액중 IGF-I과 IGFBP-3가 높은 것으로 나타났으므로, 어류 단백질 대사수준을 파악하는 지표로 활용이 가능하였다. Insulin-like growth factor-I (IGF-I) is a mitogenic peptide with a molecular mass of 7 kDa. It is produced mainly in the liver and has important functions in the regulation of development and somatic growth. Moreover, Serum IGF-I concentration is regulated by the quantity and the nutritional quality of dietary protein. To determine the IGF-I level in Korean rockfish, Sabastes schlegeli, were fed four experiment diets that contained different protein quantities, namely 30%, 40%, 50% and 60% for 70 days. Weight gain of the fish increased depending dietary protein quantity. Also, IGF-I concentrations increased according to dietary protein quantity. Feeding experiments were conducted to examine the effects of dietary protein sources on the serum IGF-I level in Korean rockfish. Fish meal (CO), soybean meal (SM), corn-gluten meal (CGM), meat meal (MM) and feather meal (FM) were used as variable protein sources of the formulated diet. IGF-I concentrations of the CO and MM groups (277.7 ±23.2, 291.5 ±41.2 ng/㎖) were higher than those of the CGM and FM groups (208.9 ±21.3, 217.2 ±38.2 ng/㎖). And IGFBP-3 levels by western blot analysis increased in good protein diets such as in the CO and MM groups. In conclusion, IGF-I may be a sensitive indicator the protein metabolism in fish as well as mammalian.
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3T3-L1 지방세포 내의 Leptin에 미치는 Polymannuronates의 영향
김인혜 ( In Hye Kim ),남택정 ( Teak Jeong Nam ) 한국수산과학회 2004 한국수산과학회지 Vol.37 No.5
N/A This study evaluated polymanuronates on the differentiation of 3T3-L1 adipocytes. Polymannuronates increased glucose utilization and reduced the accumulation of triglycerides in the cells. The differentiation showed the same results as Oil red 0 staining. Also, the polymannuronates inhibited GPDH activity as a result of the restrained adipogenesis promotion process in 3T3-L1 adipocytes. The addition of the differentiation promotion factor to 3T3-L1 promoted the differentiation of adipocytes and increased the expression of the leptin level. However the addition of polymannuronates inhibited differentiation of adipocytes and the leptin secretion level in cells by checking the leptin protein level in the culture media. As well as this, it also inhibited the transcriptional mechanism and leptin mRNA expression. These results suggest that the addition of polymannuronates improves the physiological function of 3T3-L1 cells by reducing the accumulation of triglyceride and GPDH activity, and the repressing expression of leptin at a molecular level.
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변재형,황혜정,남택정 한국수산학회 2000 한국수산과학회지 Vol.33 No.6
알길산은 3T3-Ll 세포의 분화를 억제하였다. 3T3-Ll 세포의 분화를 촉진시키는 인자로 밝혀진 IGF-I과 insulin을 이용하여 알긴산의 분화에 미치는 영향을 검토한 결과, 알긴산은 insulin의 분화촉진작용을 특이적으로 억제하였다. 본 연구는 알긴산을 이용하여 알긴산의 지질 감소효과를 세포의 수준에서 검증하고자 하였다는 점에서 그 의의가 있으며, 이에 알긴산의 분화억제효과가 일어나는 메카니즘에 관한 연구가 있어야 할 것으로 생각된다. This study examines the effects of alginic acid, a source of dietary fiber, in a glucose-derived media. In particular, we examined how the presence or absence of alginic acid affected the differentiation and triglyceride densities of 3T3-Ll cells. We established that the addition of insulin-like growth factor-I (IGF-I) to 3T3-Ll cells results in acceleration of differentiation. We sought to determine the role of alginic acid in the production of fat, by adding alginic acid to 3T3-Ll cells and examining its ability to limit or potentiate this stimulatory effects of IGF-I and IGF binding proteins. We have determined that alginic arid restricts 3T3-Ll cell differentiation and the creation of triglycerides, effectively attenuating 3T3-L1 cell metablolism and growth.
ScienceON
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