http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
- 中文 을 입력하시려면 zhongwen을 입력하시고 space를누르시면됩니다.
- 北京 을 입력하시려면 beijing을 입력하시고 space를 누르시면 됩니다.
RISS 인기검색어
- 검색키워드
- 저자 : 나경두 (검색결과 1 건)
- 무료
- 기관 내 무료
- 유료
-
배양된 망막신경절세포주에서 허혈전처치와 Protein kinase C의 역할
나경두,강성용,성공제,홍사민,전미진,김찬윤,Kyoung Doo Na,Sung Yong Kang,Gong Je Seong,Samin Hong,Mi-Jin Chun,Chan Yun Kim 대한안과학회 2008 대한안과학회지 Vol.49 No.6
Purpose: To investigate the cellular protective effects of hypoxic preconditioning against oxidative stress in a staurosporine-differentiated RGC-5 cell line and the relevance of protein kinase C subtype expression. Methods: The minimum staurosporine concentration and exposure time necessary to morphologically fully differentiate RGC-5 cells were determined. Cytotoxic injury was provided by oxidative stress with 800 μM hydrogen peroxide (H2O2) for 15 hours to morphologically fully-differentiated cells. The cytoprotective effect of hypoxic preconditioning was found by exposing the cell line to 0.3% oxygen for different periods of time. Quantifiable changes in the expression of mRNAs and proteins of the isoenzymes α, β, γ, δ, ε, ζ of protein kinase C were determined before and after 1, 2, 15, and 24 hours of hypoxic preconditioning. Results: Axonal growth in RGC-5 cells after the induction of differentiation with staurosporine caused these cells to resemble neurons. The minimal concentration and exposure time to staurosporine that evoked full differentiation of RGC-5 cells was exposure to 2 μM staurosporine for 1 hour. An LDH assay demonstrated that hypoxic preconditioning had neuroprotective effects against hydrogen peroxide-induced oxidative stress. Protein and mRNA levels of PKC isoforms α and ε increased after preconditioning. Conclusions: Hypoxic preconditioning of staurosporine-differentiated RGC-5 cells had a cytoprotective effect against oxidative stress. The associated increase of mRNA and proteins of PKC isoenzymes α and ε suggest some functional relevance of these isoenzymes to the cytoprotective effects conferred by hypoxic preconditioning. J Korean Ophthalmol Soc 49(6):979-986, 2008
KCI
스콜라연관 검색어 추천
이 검색어로 많이 본 자료
활용도 높은 자료
