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꾸지 뽕나무로부터 분리한 flavonoid 계 화합물의 암세포성장 저해 및 항산화 활성
이인경(In Kyoung Lee),송경식(Kyung Sik Song),김창진(Chang Jin Kim),김환묵(Hwan Muk Kim),오구택(Goo Taeg Oh),유익동(Ick Dong Yoo) 한국응용생명화학회 1994 Applied Biological Chemistry (Appl Biol Chem) Vol.37 No.2
Five cytotoxic and antioxidative flavonoids were isolated from the stem bark of Cudrania tricuspidata by consecutive purification using HP-20, silicagel and prep-HPLC. They were identified as taxifolin, orobol, eriodictyol, dihydrokaempferol and steppogenin by means of spectral studies. The antioxidative activities (IC_(50)) assayed by TBA method of these compound 1∼5 to were 6, 3, 3, $gt;50, and 10 ㎍/㎖, respectively. The effect on the growth inhibition (IC_(50)) of these compounds against P388 cell line were found to be 0.18, 3.3, 15 and 6.2 ㎍/㎖, respectively in the order of compound 2 to 5.
신령버섯 (Agaricus blazei) 으로부터 면역증강활성 다당류의 분리 및 화학적 특성
김환묵,유익동,조수묵,박정식,김광포 한국균학회 1999 韓國菌學會誌 Vol.27 No.2
Water-soluble polysaccharides from the fruiting bodies of Agaricus blazei Murill were extracted with 0.9% sodium chloride and hot water, successively. The purified polysaccharides showed a potent immunostimulating activity. Eight major polysaccharides, which were named from AG-1 to AG-8, were fractionated and purified by ethanol precipitation, ion exchange chromatography on DEAE-cellulose and gel filtration on Toyopearl HW 65F. These polysaccharides were identified to be homogeneous by analysis of HPLC. Three major active polysaccharides (AG-2, -3, and -6) showed relatively strong immunostimulating activity. AG-2 and -3 were composed of glucose, galactose and mannose in the molar ratios of 74.0:15.3:10.7 and 63.6:17.6:12.7, respectively. AG-6 was composed of glucose and ribose in the molar ratios of 81.4:12.6.
복령 균핵의 알칼리추출물에서 정제한 면역활성 증강물질의 작용과 화학구성
전영진,이상달,조수묵,박정식,한상배,김환묵,김광포 한국균학회 1999 韓國菌學會誌 Vol.27 No.4
An one percent sodium carbonate extract prepared from sclerotic of Poria cocos activated the proliferation of the T lymphocytes as measured by mixed lymphocyte responses(MLR). The active fraction, PCSC22, was isolated from an one percent sodium carbonate extract by a combination of fractionation procedures, including ethanol precipitation and chromatographies on column of DEAE-cellulose and Sephadex G50. Carbohydrate and peptide contained in PCSC22 were 78 : 22% in ratio. On employing gel filtration high performance liquid chromatography, PCSC22 exhitited a homogeneous peak with an average molecular weight of 8 kDa. The sugar moiety of PCSC22 was composed with mannose (92%), galactose (6.2%) and arabinose (1.3%), which might be indicated as heteromannan. Fifteen amino acids were found in peptide moiety of the polysaccharide and aspartic acid, serine, and valine were major components. PCSC22 activated the primary proliferation of T lymphocytes measured by mixed lymphocyte responses, the antibody production of the B lymphocytes and the secretion of nitric oxide from macrophage cell line, RAW264.7.