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척수통증과민반응에서 Glutamate 의 역할에 대한 분자생물학적 연구 - 포르말린 시험에서의 NMDAR 과 mGluR5 의 변화 -
김해규(Hae Kyu Kim),정진섭(Jin Sup Jung),백승완(Seong Wan Baik) 대한통증학회 2001 The Korean Journal of Pain Vol.14 No.1
N/A Background: Subcutaneous injection of 5% formalin into the hind paw of the rat produces a biphasic nociceptive response. The second phase depends on changes in the dorsal horn cell function that occur shortly after an initial C-fiber discharge, spinal sensitization, or windup phenomenon. This study was performed to investigate the role of glutamate during spinal sensitization. Methods: Sprague-Dawley rats weighing 200 to 250 g were used for this study. Under light anesthesia (0.5% isoflurane) the rats were segregated in a specially designed cage and 50 ㎕ 0.5% formalin was injected subcutaneously in the foot dorsum of right hindlimb. Forty minutes after the formalin injection, the rat was quickly decapitated and spinal cord was removed. The spinal segments at the level of L3 (largest area) was collected and stored in a deep freezer ( -70℃). The mRNA gene expression of N-methyl-D-aspartate receptor (NMDAR) and the metabotropic glutamate receptor subtype 5 (mGluR5) were determined by the polymerase chain reaction. Results: The number of flinches was 19.8 ± 2.3/min. at one minute after formalin injection and decreased to zero after then. The second peak appeared at 35 and 40 minutes after formalin injection. The values were 17.8 ± 2.2 and 17.2 ± 3.0/min. The mRNA gene expressions of NMDAR and mGluR5 were increased by 459.0 ± 46.8% (P < 0.01) and 111.1 ± 4.8% (P > 0.05) respectively at 40 minufes after formalin injection. The increased rate of NMDAR was significantly higher than that of mGluR5 (P < 0.01). Conclusions: From these results, it suggested that NMDAR partly contributed to the mechanism of central sensitization after the formalin test but mGluR5 did not.
문원배,김해규,백승완,김인세,정규섭,Moon, Won-Bae,Kim, Hae-Kyu,Back, Seong-Wan,Kim, Inn-Se,Chung, Kyoo-Sub 대한통증학회 1990 The Korean Journal of Pain Vol.3 No.1
1) 에테르-아스피린 국소도포요법은 그 방법이 간단하며 안전하게 사용할 수가 있다. 2) 이 요법은 대상포진성 신경통 환자의 통증을 반감시키나 대상포진후 신경통 환자에서는 그 효과가 입증되지 못했다. 3) 에테르-아스피린 도포는 대상포진의 피부 병변의 빠른 치유에도 도움을 주는 것으로 보인다. Herpes zoster is a common, usually self-limited disease distinguished by pain and a characteristic vesicular rash. The clinical onset of herpes zoster is heralded by pain in the area of the affected segment. So herpetic neuralgia is a major complaint from patients visiting the pain clinic. There are several methods for the treatment of herpetic neuralgia, but there is no method that results in complete remission. In 1988, King introduced the chloroform-aspirin topical application method. This method is known to be a very simple and effective treatment of acute herpetic neuralgia and postherpetic neuralgia. We used diethyl ether instead of chloroform as the solvent and treated 12 patients; 7 patients with herpetic neuralgia and 5 patients with postherpetic neuralgia. The results were follows, 1) The treatment has proved to be highly effective in relieving pain in acute herpetic neuralgia. 2) The application was very simple and safe to use and treatment tolerance has been excellent. 3) There were no effective results in postherpetic neuralgia. 4) The topical treatment seemed to promote the healing of the herpetic skin lesion.
스테로이드 사용(使用)으로 인(因)한 고혈당증(高血糖症)을 동반(同伴)한 대상포진후(帶狀疱疹後) 신경통(神經痛) 환자(患者)의 치험(治驗)
김영추,김해규,백승완,김성수,김인세,정규섭,Kim, Young-Choo,Kim, Hae-Kyu,Baik, Seong-Wan,Kim, Sung-Soo,Kim, Inn-Se,Chung, Kyoo-Sub 대한통증학회 1990 The Korean Journal of Pain Vol.3 No.1
대상포진후 신경통의 치료를 위하여 사용하였던 스테로이드로 인한 고혈당증을 동반한 환자를 치료한 결과를 다음과 같이 요약한다. 첫째, 통증치료실에서 흔히 쓰이는 약물 중에는 고혈당증을 유발하는 것이 많으므로 처치전에 고혈당이 영향을 주는 다른 질환의 유무를 확인하도록 한다. 둘째, 스테로이드 사용으로 인하여 발생되는 일시적인 고혈당중의 경우에는 스테로이드 사용 중단으로 회복이 가능하나 잠재되어 있던 당뇨병의 발현시는 지속적인 당뇨병의 치료를 필요로 한다. 셋째, low level laser therapy는 혈당치에 영향을 미치지 않는 통증치료법으로 유용하게 사용될 수 있다. There are many methods for treatment of post-herpetic neuralgia (PHN) and of these, epidural steroid or intralesional steroid injection is one of the most effective treatments. But there are some problems in the use of steroids. One of which is steroid-induced hyperglycemia. In that case, the treatment of PHN is very difficult and not so effective. So we used the low level laser therapy (LLLT), He-Ne and IR, for the treatment of post-herpetic neuralgia without any changes of blood glucose level. It seemed that LLLT was a very effective method for PHN without any systemic changes.
백서에서 Gabapentin 전신투여가 Facial Formalin Test에 미치는 영향
김철홍,백승완,김해규,권재영,김경훈,최성환,Kim, Chul-Hong,Baik, Seong-Wan,Kim, Hae-Kyu,Kwon, Jae-Young,Kim, Kyoung-Hun,Choi, Sung-Hwan 대한치과마취과학회 2003 Journal of Dental Anesthesia and Pain Medicine Vol.3 No.2
Background: Gabapentin is a novel anti-epileptic drug, which is used in clinical practice to treat epilepsy. This drug is also used as an analgesic in pain patients. The antinociceptive effect of this drug was assessed using the formalin test in the rat. Methods: In order to investigate the effects of gabapentin on the trigeminal nerve territory, we injected 0.5% formalin into the upper lip. Adult, male, Sprague-Dawley rats received a $50{\mu}l$ subcutaneous injection of 5% formalin into one vibrissal pad and the consequent, facial grooming behavior was monitored. Consistent with previous investigations using tile formalin model, animals exhibited biphasic nocifensive grooming (phase 1, 0-12 min; phase 2, 12-60 min). Results: The intraperitoneal administration gabapentin 5 minutes prior to the formalin injection led to a significant, dose-dependent reduction in grooming time during phase 2. In high doses, gabapentin also reduced the time of grooming during phase 1. Conclusions: The Intraperitoneal injection of gabapentin has an analgesic effect in the facial formalin rat model and this analgesic effect increases dose-dependently.
톨루엔 흡입 흰쥐의 선조체에서 미세투석법을 이용한 세포외성 흥분성 아미노산 신경전달물질의 측정
백승경(Seung Kyung Baeck),김해규(Hae Kyu Kim),김철민(Cheol Min Kim) 대한약학회 1998 약학회지 Vol.42 No.1
Male Sprague-Dawley rats were exposed to the toluene at 3,000+/-200ppm via inhalation for two hours (single inhalation group), three weeks by two hours per day, six days per week (repeated inhalation group). We examined the level of excitatory amino acids of the extracellular neurotransmitter within the corpus striatum of rats by using in vivo microdialysis. Aspartate (Asp) and glutamate (Glu) of excitatory amino acid neurotransmitters were generally decreased in the inhalation groups compared with the control group, and more significantly decreased in the repeated inhalation group than in the single inhalation group except that Asp was increased from 60 min after the beginning of the inhalation to 30 min after the termination.
흰쥐에서 양막 유래 줄기세포가 Lipopolysaccharide 정주에 따른 염증반응에 미치는 영향
윤지영 ( Ji Young Yoon ),김해규 ( Hae Kyu Kim ) 대한마취과학회 2009 Korean Journal of Anesthesiology Vol.57 No.1
Background: Systemic inflammatory response syndrome (SIRS) and sepsis are one among the most common causes of death in intensive care units. Tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6) and interleukin-10 (IL-10) increase during SIRS and sepsis. Recent studies have demonstrated that amnion derived stem cells have anti-inflammatory effects, low immunogenicity and pluripotency. In addition, there is little ethical objection to the use of amnion derived stem cells. Here, the author presents the first study demonstrating the therapeutic potential for the use of amnion derived stem cells in a rat model of SIRS and sepsis. Methods: Amnion derived stem cells were isolated from amnion obtained from an uncomplicated Cesarean section and cultured. SIRS and sepsis were induced in rats by lipopolysacchride (15 mg/kg, LPS), and amnion derived stem cells were then transplanted intravenously. The heart rate, mean arterial pressure and the concentration of TNF-α, IL-6 and IL-10 were assessed at baseline and 2 hours and 4 hours after procedure. Results: The changes of heart rate and mean arterial pressure were reduced in the amnion derived stem cells transplanted group. In addition, increases in the plasma concentration of TNF-α, IL-6 and IL-10 were attenuated in the amnion derived stem cells transplanted group. Conclusions: This study demonstrated that amnion derived stem cells attenuate inflammatory response during SIRS and sepsis. Transplantation of amnion derived stem cells can be a novel cell based therapeutic strategy for SIRS and sepsis (Korean J Anesthesiol 2009; 57: 88~95)