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김정석,강명창,김전하,정연식,이종환,Kim, Jeong-Suk,Kang, Myung-Chang,Kim, Jeon-Ha,Jung, Youn-Shick,Lee, Jong-Hwan 한국기계가공학회 2003 한국기계가공학회지 Vol.2 No.3
The high speed machining technology has been improved remarkably in die/mold industry with the growth of parts and materials industries. Though the spindle speed of machine tool increases, the condition monitoring techniques of the machine tool, tool and workpiece in high speed machining ate incomplete. In tins study, efficient sensing technology in high speed machining is suggested by observing the characteristics of cutting force, gap sensor and accelerometer signal also, machinability of high-speed machining is experimentally evaluated sensing technique to monitor the machine tool and machining conditions was performed.
한국산 Juniperus rigida 의 두 산지의 핵형분석
김정석,정우규,안중극,정미정,한창숙 ( Chung Suk Kim,Woo Kyu Chung,Joong Kug Ahn,Mee Jeong Jeong,Chang Sook Han ) 한국산림과학회 1986 한국산림과학회지 Vol.73 No.1
Karyotypes are described for Juniperus rigida Sieb. et Zucc. in two provenances of Gyeong-nam and Choong-puk. Chromosome numbers of two provenances, are 2n=22. The most common feature of mitotic chromosomes was shown at the chromosome 7, which has secondary constriction on the short arm. And the most differential chromosome was shown at chromosome 9 from Gyeong-nam and chromosome 5 from Choong-puk provenance, which bore secondary constriction. The karyotype formulae are as follows; Gyeong-nam, Jinyang pravenance race is K(2n) = 22 = 2 A^m + 2B^m + 2C^m + 2D^(sm) + 2E^(st) + 2F^m + 2^(sc)G^m + 2H^m + 2^(sc)I^t + 2J^(st) + 2K^m Choong-puk, Jechun provenance race is K(2n) = 22 = 2A^(rn) + 2B^m + 2C^m + 2D^(st) + 2^(sc)E^(sm) + 2F^m + 2^(sc)G^m + 2H^m + 2I^m + 2J^(st) + 2K^(sm)
Juniperus chinensis 7재배종과 Pinus densiflora 1재배종에 대한 핵형분석
김정석,김영두,정우규 ( Chung Suk Kim,Yeung Du Kim,Woo Gyu Jeong ) 한국산림과학회 1986 한국산림과학회지 Vol.75 No.1
This study was designed to investigate the karyotype analysis of Juniperus chinensis and its seven varieties, and F. densiflora and P. densiflora for. multicaulis. Following results were obtained. 1. Three varieties, J. chinensis v. kaizuka, J. chinensis v. aureo-variegata and J. chinensis v. procurnbens are turned out as tetraploids. 2. Varieties having many long chromosomes and long mean relative length of chromosomes are J. chinensis and J. chinensis v. aureo-globosa, while varieties having short chromosomes are J. chinensis v. horizontalis and J. chinerssis v. globosa. 3. Varieties with high mean ratio of long arm length to short arm are J. chinensis v. globosa and J. chinensis v. kaizuka, while a variety with the low mean ratio is J. chinensis v. aureo-globosa. 4. When chromosomes are arranged according to the Total length, the most similar variety with J. chinensis was J. chinensis v. sargentii, J. chinensis v. horizontalis, J. chinensis v. globosa and J. chinensis v. aureo-globosa, while the least similar one was J. chinensis v. procumbens. 5. Pinus densiflora for. multicaulis has shorter mean relative length of chromosome than P. densiflora, while the arm ratio of the former is higher than the tatter. 6. When chromosomes are arranged according to the total length, six ctuomosomes showed the same order between the two varieties. 7. P. densiflora for. multicaulis has many chromosomes with secondary constriction.
복합 신경회로망을 이용한 채터진동의 인프로세스 감시(II)
김정석,강명창,박철,Kim, Jeong-Suk,Kang, Myeong-Chang,Park, Cheol 한국정밀공학회 1995 한국정밀공학회지 Vol.12 No.12
The In-process minitoring of the chatter vibration is necessarily required to an automatic manufacturing system. In this study, we constructed a multi-sensing system using tool dynamoneter, accelerometer and AE(Acoustic Emission) sensor for a more credible detection of chatter vibration. And a new approach using a multiple neural network to extract the features of multi-sensor for the recognition chatter vibration is proposed. With the Back-propagation training process, the neural network memorize and classify the features of multi-sensor signals. As a result, it is shown by multiple neural network that the chatter vibration can be monitored accurately, and it can be widely used in practical unmanned system.
원문 : Dexamethasone 및 전기 자극 처치 시 근세포로부터 유발된 exosome의 항 근위축 및 근비대 관련 microRNA 및 단백질의 변화에 미치는 효과
김정석 ( Jeong Suk Kim ),박소영 ( So Young Park ),윤정수 ( Chung Su Yoon ),박대령 ( Dae Ryoung Park ) 한국운동생리학회(구 한국운동과학회) 2014 운동과학 Vol.23 No.4
본 연구의 목적은 골격근 세포에 dexamethasone (Dex) 및 전기 자극(ES) 처치 시 발생하는 microRNA(miR)가 각각의 자극에 따라 어떠한 profile을 가지게 되며, 이러한 exosome을 골격근 primary cell에 처치 시 근위축 및 근비대 관련 인자의 인산화가 어떻게 변하는지를 규명하기 위한 것이었다. C2C12 세포주에 Dex 및 ES를 처치하여 골격근 세포로부터 유리된 exosome의 miR의 profile을 분석하였으며, 이러한 과정에서 얻어진 exosome을 골격근 primary cell에 재처리하여 골격근 세포에서의 근위축 및 근비대 관련 인자의 인산화 변화를 관찰하였다. C2C12 세포주에 Dex 혹은 ES를 처치한 결과, 근위축 관련 miR인 miR-1, miR-206, miR-322와 근비대 관련 miR-23a의 발현 양상이 유의하게 차이나는 것을 확인하였다. 또한 이러한 세포에서 얻어진 exosome을 골격근 primary cell에 처치한 결과, Dex를 처치한 세포에서 얻어진exosome은 근위축 관련 인자인 FOXO와 MuRF의 인산화를 증가시키는 것에 비해 ES를 처치한 세포에서 얻어진 exosome은 근비대관련인자인 mTOR와 p70S6K의 인산화를 증가시키는 것으로 나타났다. 이러한 결과는 각각의 신호체계에 의해 발생되는 골격근내 exosome이 세포의 신호전달 체계에 중요한 역할을 하고 있음을 제시하고 있으며, exosome에 의한 miR의 전달이 근위축 및 근비대와 관련된 신호단백질의 인산화를 조절할 수 있음을 제시하고 있다. The purpose of this study was to examine the effect of dexamethasone (Dex) treatment and electrical stimulation (ES) on skeletal muscle cell derived exosome`s microRNA (miR) profile and its effect on skeletal muscle signal protein expression. Exosome obtained from two different C2C12 cell were treated with Dex or ES. Then we profiled exosome`s miR expression and atrophy related miR-1, miR-206, miR-322 were significantly increased with DEX however, hypertrophy related miR-23a was significantly increased only in the ES. Further we treated two different types of exosome to Dex treated skeletal muscle primary cell and two types of exosome differently regulated atrophy related protein (FOXO/MurF) and hypertrophy related protein (mTOR/p70S6K) phosphorylaton. In conclusion, the results indicate that atrophy or hypertrophy signal induces different exosome including different profile of miR and it plays important role in skeletal muscle atrophy and hypertrophy.