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- 저자 : 김의홍(EH Kim) (검색결과 4 건)
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서병화(BH Shu),김의홍(EH Kim),김영(Y Kim),한경학(KH Han) 대한산부인과학회 1981 Obstetrics & Gynecology Science Vol.24 No.8
This study is clinico-statistical analysis and survey of 260 cases who were admited and treated for ectopic pregnancy at Soonchunhyang Hospital from June 1, 1974 to May 31, 1980 The results of this study were as follows; 1. The incidence of ectopic pregnancy was 1 : 34 (3.0%) of deliveries and tends to increase the number of cases every year. 2. The most freguent age group was in 25-39 years of age (81.5%) and the mean age was 31.5 years. 3. Nulligravida was 13.8% nullipara 29.2% and multipara 70.8% and the cases who have experienced artificial abortion ever one time was 65.4% and the last pregnancy ended in normal delivery in 21.2% and artificial abortion in 48.8% of total 4. The mean duration of sterility was 3 years and 4 months and 7.3% was 9 years or more of sterility. 5. A history of previous artificial abortion was noted in 65.4% and previous laparotomy in 19.2% among them, 8.1% was repeat ectopic pregnancy and 5.4% was appendectomy laparoscopic sterilization was 3.8% Pelvic inflammatory disease was noted in 4.6% vaginitis in 3.5% pelvic tuberculosis in 1.5% IUD in 1.5% and cervicitis in 1.2%. The duration between the first and the second ecopic pregnancy was distributed in 3 months to 12 years and its mean was 4 years and 6 months. Particullary one case of third ectopic pregnancy on same side was present. 6. The mean interval between L.N.M.P and onset of symptoms was 6.2 weeks.
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정경환(KH Chung),서희숙(HS Suh),김의홍(EH Kim),이영주(YJ Lee),박세웅(SW Park),김원배(WB Kim),이동화(DH Rhee),강득용(DY Kang) 대한산부인과학회 1982 Obstetrics & Gynecology Science Vol.25 No.7
저자들은 Spiegelberg`s criteria를 충족시키는 난소임신 3예를 본병원에서 1974년 5월부터 1980년 12월 30일까지 약 7년간에 3예를 경험하였기에 문헌적 고찰과 함께 보고하는 바이다. Primary ovarian pregnancy is still a comparatively rarity in ectopic pregnancy, which fullfilled Spiegelberg`s criteria for the diagnosis of ovarina pregnancy. Three cases of ovarian pregnancy are reported and a brief review of literature was made.
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정하영(HY Chung),서준(J Suh),김의홍(EH Kim),이순덕(SD Lee) 대한산부인과학회 1981 Obstetrics & Gynecology Science Vol.24 No.9
Inversion of the uterus is a rare obstetric complication and emergency. We present a case of acute puerperal incomplete inversion of the uterus with a brief review of the literature.
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Flow cytometry를 이용하여 자궁내막암에 대한 단주항체 MSN-1의 반응성에 관한 연구
조태호,김의홍,이순곤 대한산부인과학회 1998 Obstetrics & Gynecology Science Vol.41 No.6
현재 flow cytometry를 암진단에 응용하는 경우에는 핵내의 DNA 양을 측정하는 것이 주로 행해지고 있지만 자궁내막암에서는 이배체세포가 많은 것으로 되어 있기 때문에 DNA의 정량만으로는 암의 확정진단이 어려울 것으로 생각된다. 그리하여 종양표지물질에 촛점을 돌려 기존의 종양표지물질의 존재를 조직 절편을 이용하여 면역조직화학적으로 검토해 보았으나 자궁내막암에 특히 높은 양성률을 나타내는 것은 볼 수가 없었다. 본 연구에서는 자궁내막선암 세포주인 SNG-Ⅱ를 면 역원으로 하여 만든 단주 항체인 MSN-1을 이용하여 자 궁내막암에 대한 단주 항체 MSN-1의 반응성을 flow cytometry에 의해 검토해 보았다. MSN-1의 면역원인 자궁내막선암세포주 SNG-Ⅱ와 MSN-1의 반응률은 78.8%였으며 뚜렷하고 넓은 형광 정 점이 형성되었고 MSN-1은 자궁경부암 세포주 SK G-Ⅲ a와는 반응성을 나타내지 않았다. 조직의 결과를 보면 정상 자궁내막에서는 9예 중 1예 에서만 79%였으며 나머지 8예에서는 30% 이하의 양성 률을 나타내는 데 반하여 자궁내막암에서는 6예 중 6예 가 30% 이상의 높은 양성률을 나타내었다. 자궁내막암 에서는 자궁내막암 세포주인 SNG-II와 거의 비슷한 양상을 보였으며, 자궁내막암의 증례에서 모두 같은 그래프 양상을 보여주었다. 이와 같은 결과로 MSN-1이 정상 자궁내막세포와 자 궁내막암 세포를 감별하는 하나의 지표가 될 수 있는 가능성을 시사해 주었다. In the cytology, we sometimes have difficulties to make definitive diagnosis of endometrial cancers. In order to devise a new supplementary method, we used anti-endometrial adenocarcinoma monoclonal antibody MSN-1. In immunohistochemistry, MSN-1 reacted with more than 80% of endometrial adenocarcinomas. So, we analyzed the reactivities of MSN-1 with normal endometrium and endometrial adenocarcinoma by flowcytometry. First, in order to find out optimal conditions of sample treatment, we carried out some fundamental experiments using cultured SNG-II cell line which was the immunogen of MSN-1. They were clarified that mouse IgM had to be used as a control in every sample in order to subtract nonspecific reaction, and that the cell damage might be reduced by using collagenase dissolved in tissue culture medium. In clinical samples, 0.1% collagenase was used to disaggregate tissues, and cell suspensions were stained by indirect immunofluorescence technique. After filtered through 50 ㎛ nylon mesh, cells were analyzed by the flowcytometer FACscan (Becton Dickinson). All of 6 cases of endometrial adenocarcinomas showed positive rate of more than 30%, while 8 of 9 normal endometrium cases were under 30%, indicating that the application of MSN-1 to flowcytometry might be useful to differentiate endometrial cancer cells from normal ones.
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