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- 저자 : 김의경(Euikyung Kim) (검색결과 5 건)
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하대식,김충희,김의경,강정부,김종수,Hah, Dae-Sik,Kim, Chung-Hui,Kim, Euikyung,Kang, Chung-boo,Kim, Jong-shu 대한수의학회 2007 大韓獸醫學會誌 Vol.47 No.1
Houttuynia cordata root on non-lipid oxidative damage. The antioxidative efects of methanolic (MeOH) extract of Houttuynia cordata rooton non-lipid, including liposome oxidation, oxidation of deoxyribose, protein oxidation, chelating, scavenging,and 2'-deoxyguanosine (2'dG) oxidation were investigated. Houttuynia cordata root exhibited highantioxidative effect in a liposome model system. The inhibitory effect of MeOH extract on deoxyribosedamage exhibited antioxidative effect and it afforded considerable protection against damage to deoxyribose.In addition, MeOH extract at over 300extracts exhibited metal binding ability for hydrogen peroxide. Furthermore, the oxidation of 2'dG to 8-hydroxy-2-deoxyguanosine was inhibited by MeOH extracts, and scavenging activity for hydroxyl radicalexhibited a remarkable effect. The present results on biological model systems showed that MeOH extractswas effective in the protection of non-lipids against various oxidative model systems.
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Skin sensitization test of potassium hydroxide in guinea pigs
황두현(Du Hyeon Hwang),김석(Suk Kim),김의경(Euikyung Kim),이후장(Hu-Jang Lee) 한국예방수의학회 2020 예방수의학회지 Vol.44 No.4
This study was to evaluate the skin sensitization of potassium hydroxide (KOH) by the guinea pig maximization test. In the preliminary test, the induction and challenge dose of KOH were determined as 1.0 and 0.2%, respectively. Guinea pigs were sensitized with intradermal injection of 0.1ml KOH for 24 h. After 1 week, 1.0% of KOH was treated on the site of injection and 0.2% of KOH was challenged 2 weeks later. The average skin reaction evaluated by erythema and edema on the challenge sites and sensitization rate in the KOH-treatment group at 24 h were 0.0 and 0%, respectively, which are substantially low compared with in the 2,4-dinitrochlorobenzene-treatment group (positive control group) (average skin reaction: 3.0, sensitization rate: 100%) and identical with in the distilled water-treatment group (negative control group), representing a weak sensitizing potential. The average skin reaction and sensitization rate observed at 48 h were 0.0 and 0% in the KOH-treatment group, respectively, and 2.0 and 100% in the positive control group, respectively. It was concluded that 1.0% of KOH classified to Grade I, induced no sensitization when tested in guinea pigs.
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강우침식인자 도출을 위한 강우에너지 산정 방법과 결과의 다양성에 관한 연구
황세운 ( Syewoon Hwang ),김동현 ( Donghyeon Kim ),신상민 ( Sangmin Shin ),장민원 ( Minwon Jang ),김의경 ( Euikyung Kim ) 한국농공학회 2016 한국농공학회 학술대회초록집 Vol.2016 No.-
범용토양유실공식(RUSLE)은 연간 토양유실량을 산정하기 위해 제시된 경험식이며, 강우침식인자(R factor)는 강우강도의 특성을 고려하는 RUSLE 주요인자이다. 국내 실험을 통해 강우에너지 기반 침식인자 산정법이 개발된 바 있으며 현재까지 간편법을 비롯한 다양한 공식들이 적용되고 있다. 본 연구에서는 강우침식인자를 산정하는 과정에서 다른 강우 운동에너지식을 적용하거나 연평균 강수량 등을 대체지수로 활용한 간편법 적용시 결과의 다양성을 분석하고자한다. 합리적 강우침식인자 산정을 위해 72개 종관기상관측 지점에 대한 분단위 강우자료(1998~2014)를 수집하고 기존의 국내외 강우운동에너지 식과 대체지수를 적용하여 산정된 결과를 비교·분석하였다. 연구결과 간편법 적용시 대부분 지점에 대해 강우에너지식을 사용한 강우침식인자보다 과대산정(지점평균 약 74%)하였으며 다른 강우에너지식 적용에 따른 평균 변동계수가 약 0.12로 나타나 지점 간 차이를 보였으며 적용방법에 따른 침식인자의 공간분포 또한 다르게 나타남을 확인하였다. 지형·지역적 양상이 다르게 나타나는 토양유실량 예측에 있어 강우침식인자 산정을 위한 최적 방법론 선정 및 개발은 한계가 있는 만큼 다중모델 결과를 조합하는 방법론 개발과 신중한 적용이 필요하다고 판단된다.
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양혜련(Hyeryeon Yang),이예현(Ye Hyeon Lee),성종배(Jong Bae Seong),최석문(Suk Moon Choi),황두현(Du Hyeon Hwang),김의경(Euikyung Kim),강창근(Changkeun Kang) 한국예방수의학회 2020 예방수의학회지 Vol.44 No.4
Jellyfish envenomation is a world-wide health problem, which often seriously affect the fishery and bathing activities. To date, few individual jellyfish venoms proteins have been thoroughly characterized yet. In this work, four species of scyphozoan jellyfish (Nemopilema nomurai, Cyanea nozakii, Aurellia aurita and Rhopilema esculentum) are compared according to their, cytotoxicity, hemolytic potency, brine shrimp toxicity and protein components. Jellyfish venoms showed higher cytotoxicity in H9C2 heart myoblast than in C2C12 skeletal myoblast, with the exception of C. nozakii venom. This result suggests that the selective cytotoxic effects may be possibly related to their in vivo effects of cardiac tissue dysfunction. On the other hand, hemolytic activity could be also observed from all tested jellyfish venoms. N. nomurai jellyfish venom displaying the greatest hemolytic activity. As an alternative method of evaluating the toxicities of jellyfish venoms, the toxicity on brine shrimp was examined with the four jellyfish venoms. From this, the venom of N. nomurai showed higher toxicity against brine shrimp than the other jellyfish venoms, which is consistent with the results of cytotoxicity assay as well as hemolysis assay of the present study. SDS-PAGE analysis of four jellyfish venoms showed the similar pattern with molecular weight of around 40 kDa, and appeared to be the major protein components. These results provided that N. nomurai jellyfish venom was potently toxic than other scyphozoan jellyfish venoms and may explain to some extent the deleterious effects associated with human envenoming.
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Skin Sensitization Test of Chamaecyparis obtusa essential oil in Guinea Pigs
Du Hyeon Hwang(황두현),Eun-Kee Park(박은기),Chang-Yeul Yoo(유창열),Suk Kim(김석),Euikyung Kim(김의경),Hu-Jang Lee(이후장) 한국예방수의학회 2019 예방수의학회지 Vol.43 No.3
Despite the various effects on the health of Chamaecyparis obtusa essential oil (COEO), an adverse effect on the skin has recently been reported. Therefore, in this study, the skin sensitization test of COEO was conducted to confirm the skin safety of the essential oil by the guinea pig maximization test. Guinea pigs were sensitized with intradermal injection of 0.1ml COEO for 24 h. After 1 week, 5% of COEO was treated on the site of injection and 2% of the essential oil was challenged 2 weeks later. The average skin reaction evaluated by erythema and edema on the challenge sites and sensitization rate in the COEO-treatment group at 24 h were 0.0 and 0%, respectively, which are substantially low compared with in the 2,4-dinitrochlorobenzene-treatment group (positive control group) (average skin reaction: 3.0, sensitization rate: 100%) and identical with in the olive oil-treatment group (negative control group), representing a weak sensitizing potential. The average skin reaction and sensitization rate observed at 48 h were 0.0 and 0% in the COEO-treatment group, respectively, and 2.0 and 100% in the positive control group, respectively. It was concluded that 5% of COEO classified to Grade I, induced no sensitization when tested in guinea pigs.
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