심장내과 병동의 텔레메트리 적용 환자 특성

김윤선,최혜란,Kim, Yoon-Seon,Choi, Hye Ran 한국중환자간호학회 2014 중환자간호학회지 Vol.7 No.2

Purpose: The purpose of this study was to provide basic information to develop appropriate nursing guidelines for cardiac telemetry. Methods: This retrospective research was conducted to identify the current usage of cardiac telemetry and considered 1,000 patients hospitalized for telemetry. The collected data were analyzed using IBM (SPSS Statistics for Windows 21.0). Results: Four-hundred and ninety-two patients (49.2%) were diagnosed with arrhythmia and 209 (20.9%) with heart failure. Electrocardiogram (ECG) rhythm changes were detected via telemetry in 464 cases. Major arrhythmias were ventricular tachycardia (183, 39.4%) and bradycardia (99, 21.3%). Interventions after detecting arrhythmia were cardioversion (16, 3.4%), defibrillation (1, 0.2%), and cardiopulmonary cerebral resuscitation (5, 1.1%); other patients were treated conservatively with close observation (381, 82.1%). Conclusion: There was appropriate intervention in 46.4% of the considered cases with the detection of ECG changes before patient notification, which implied that cardiac telemetry could be considered for application to patients with hemodynamic instability. Clear standards and guidelines are required to determine who requires telemetry and when to end the telemetry monitoring.

난자-난구세포 복합체에서 발현하는 Rpia 유전자의 종 특이적 발현

김윤선,윤세진,김은영,이경아,Kim, Yun-Sun,Yoon, Se-Jin,Kim, Eun-Young,Lee, Kyung-Ah 대한생식의학회 2007 Clinical and Experimental Reproductive Medicine Vol.34 No.2

목 적: 본 연구진은 선행연구를 통하여 생쥐의 미성숙 난자와 성숙 난자 사이에 차이 나게 발현하는 유전자(DEGs)의 목록을 보유하고 있는데, 그 중에서 pentose phosphate pathway (PPP)에 필수적 효소인 Ribose 5-phosphate isomerase A (Rpia)를 선택하여 본 연구를 수행하였다. 난자 성숙 과정에 관련된 Rpia의 기능을 알아보기 위한 기초연구로서 생쥐와 돼지의 난소에서 Rpia의 발현을 비교분석 하였다. 연구방법: 생쥐의 각 조직에서 11개의 MII-selective DEGs의 발현을 RT-PCR방법으로 확인하여 난소에서 강하게 발현하는 4개의 유전자를 선택하였고, 다시 이들 4개 유전자 중 난자에서 높게 발현하는 Rpia를 선택하여 생쥐 및 돼지의 난자, 난구세포, 과립세포에서의 발현을 비교분석 하였다. 돼지 Rpia 염기서열은 밝혀져 있지 않아 EST clustering 기법을 통해 동정하였다. 결 과: EST clustering 기법으로 찾아낸 돼지 Rpia 염기서열은 GenBank에 등록하였고 (Accession Number EF213106), 이를 근거로 primer를 작성하여 RT-PCR을 수행하였다. Rpia 유전자는 생쥐에서는 난자 특이적으로 발현하는 반면 돼지에서는 난자, 난구세포, 과립세포에서 모두 발현하는 차이점을 발견하였다. 결 론: 본 연구는 생쥐와 돼지의 난소에서 Rpia유전자 동정에 대한 첫 보고로서, 본 연구결과로부터 생쥐와 돼지의 COCs는 서로 다른 경로로 포도당의 대사가 일어나는 것을 알 수 있었다. 따라서 이와 같은 차이점이 두 종의 난자를 체외 배양할 때 나타나는 난자 성숙률의 차이를 가져오는 기전 중의 하나가 아닐까 추측된다. 난자 성숙을 조절하는 기전을 연구함과 동시에 체외에서 난자 성숙이 어려운 종의 최적의 IVM (in vitro maturation)조건을 찾기 위해서는 앞으로 난자와 주변세포의 포도당 대사과정에 미치는 Rpia의 기능에 대한 후속연구가 필요할 것으로 사료된다. Objective: We previously identified differentially expressed genes (DEGs) between germinal vesicle (GV) and metaphase II (MII) mouse oocyte. The present study was accomplished as a preliminary study to elucidate the role of ribose 5-phosphate isomerase A (Rpia), the essential enzyme of the pentose phosphate pathway (PPP), in oocyte maturation. We observed expression of Rpia in the mouse and porcine oocytes. Methods: Expression pattern of the 11 MII-selective DEGs in various tissues was evaluated using RT-PCR and selected 4 genes highly expressed in the ovary. According to the oocyte-selective expression profile, we selected Rpia as a target for this study. We identified the porcine Rpia sequence using EST clustering technique, since it is not yet registered in public databases. Results: The extended porcine Rpia nucleotide sequence was submitted and registered to GenBank (accession number EF213106). We prepared primers for porcine Rpia according to this sequence. In contrast to the oocyte-specific expression in the mouse, Rpia was expressed in porcine cumulus and granulosa cells as well as in oocytes. Conclusion: This is the first report on the characterization of the Rpia gene in the mouse and porcine ovarian cells. Results of the present study suggest that the mouse and porcine COCs employ different mechanism of glucose metabolism. Therefore, the different metabolic pathways during in vitro oocyte maturation (IVM) in different species may lead different maturation rates. It is required to study further regarding the role of Rpia in glucose metabolism of oocytes and follicular cell fore exploring the regulatory mechanism of oocyte maturation as well as for finding the finest culture conditions for in vitro maturation.

B cells transduced with HPV16 E6/E7-expressing adenoviral vector can efficiently induce CTL-dependent anti-tumor immunity

김윤선,고현정,Yeon-Jeong Kim,Seung-Hee Han,Jung-Mi Lee,Woo-Sung Chang,Hyun-Tak JIN,Young-Chul Sung,강창율 대한면역학회 2007 Immune Network Vol.7 No.3

Background: Human papillomavirus (HPV) infection is responsible for cervical cancer, a common cancer in women. Since HPV infection and cancer development are controlled by the host immune system, immunotherapy against HPV can be helpful in preventing or treating HPV-associated cervical cancer. Two oncoproteins of HPV16, E6 and E7, are promising targets for immunotherapy against cervical cancer, because they are constitutively expressed in cervical cancer. Methods: Since cellular vaccines using B cells as well as dendritic cells offer an efficient approach to cancer immunotherapy, we opted to use B cells. We evaluated the immunogenicity and anti-tumor effects of a B cell vaccine transduced with HPV16 E6/E7-expressing adenovirus. Results: Vaccination with HPV16 E6/E7-transduced B cells induced E6/E7-specific $CD8^+$ T cell-dependent immune responses and generated anti-tumor effects against E6/E7-expressing TC-1 tumor. The anti-tumor effect induced by this B cell vaccine was similar to that elicited by DC vaccine, showing that B cells can be used as an alternative to dendritic cells for cellular vaccines. Conclusion: Thisstudy has shown the feasibility of using B cells as immunogenic APCs and the potential for developing prophylactic and therapeutic vaccines against HPV-associated cervical cancer using a B cell vaccine transduced with adenovirus expressing HPV16 E6/E7.

현장일기-나의 일터

김윤선,Kim, Yun-Seon 대한산업보건협회 1986 산업보건소식 Vol.32 No.-

표준 기술동향 - LTE-Advanced 기반 전송지점 간 협력통신에 대한 표준화 동향 및 성능 분석

김윤선,이효진,김영범,이주호,Kim, Yun-Seon,Lee, Hyo-Jin,Kim, Yeong-Beom,Lee, Ju-Ho 한국정보통신기술협회 2012 TTA저널 Vol.139 No.-

페닐프로피온산계 해열진통제 고형지질나노입자의 입도분포와 약물봉입 및 용출특성

김윤선,김길수,Kim, Yoon-Sun,Kim, Kil-Soo 한국약제학회 1998 Journal of Pharmaceutical Investigation Vol.28 No.4

Solid Lipid Nanoparticle(SLN), one of the colloidal carrier systems, has many advantages such as good biocompatibility, low toxicity and stability. In this paper, the effects of drug lipophilicity and surfactant on the drug loading capacity, particle size and drug release profile were examined. SLNs were prepared by homogenization of melted lipid dispersed in an aqueous surfactant solution. Ketoprofen, ibuprofen and pranoprofen were used as model drugs and tweens and poloxamers were tested for the effect of surfactant. Mean particle size of prepared SLNs was ranged from 100 to 150nm. The drug loading capacity was improved with the most lipophilic drug and low concentration of surfactant. Particle size and polydispersity of SLNs were changed according to the used lipid and surfactant. The rates of drug release were controlled by the loading drug and surfactant concentration. SLN system with effective drug loading efficiency and proper particle size for the intravenous or oral formulation can be prepared by selecting optimum drug and surfactant.

유방암 생존자의 삶의 질 영향요인

김윤선(Kim Yoon Sun),태영숙(Tae Young Sook) 대한종양간호학회 2011 Asian Oncology Nursing Vol.11 No.3

Purpose: This study was aimed to identify the influencing factors on the quality of life among breast cancer survivors. Methods: The subjects were 159 female patients who visited out-patient department (OPD) after the mass removal surgery for breast cancer and had completed adjuvant treatments such as chemotherapy radiation therapy at a university hospital and a general hospital. Data collection was conducted using the Ferrell QOL scale the Mishel uncertainty scale the Fitts & Osgoods body image scale revised by Jeon & Kim. the Rosenberg self-esteem scale and the Kang family support scale. Results: The level of QOL in the participants was in the middle. There were a significant correlation between QOL uncertainty self-esteem and family support. There were significant differences in QOL with the perceived health condition and the best support person. In a regression analysis the most powerful predictor of QOL was body image (21.7%). Altogether uncertainty and perceived health condition explained 28.6% of the variance of QOL of the participants. Conclusion: Body image uncertainty and perceived health condition were important predictors of QOL. These results demonstrated the need for developing interventions to improve QOL of breast cancer survivors.

Causal Knowledge Integration Method for Product Design Simulation

김윤선,권오병,Kim, Yun Seon,Kwon, Ohbyung The Korea Society for Simulation 2014 한국시뮬레이션학회 논문지 Vol.23 No.4

제품 설계를 위한 시뮬레이션을 위해서는 다양한 인과지식이 필요하다. 따라서 현존하는 다양한 지식으로부터 새로운 지식을 획득하기 위해서는 이러한 지식들을 통합하는 것이 필요하다. 예를 들어, 사용자가 가열이 되는 컵을 설계하고자 하지만 기존의 설계 지식 베이스에는 가열되는 컵에 대한 설계 지식은 없고 가열 기구에 대한 지식과 컵에 대한 지식이 따로 존재한다. 이러한 상황에서 가열 기구에 대한 지식과 컵에 대한 지식을 통합할 수 있는 자동화된 방법론이 필요하다. 이를 통해 사용자는 가열이 되는 컵을 설계하는 지식을 획득할 수 있다. 따라서 본 연구의 목적은 이러한 제품 설계에 관련한 지식을 통합하여 새로운 지식을 만드는 방법론을 제안 한다. Simulation for product design requires a lot of causal knowledge. Hence, knowledge integration is required for obtaining a new knowledge from existing knowledge. For example, a user requests knowledge for the heating cup. However, the knowledge base only has knowledge for heating and cup, not heating cup. At his situation, knowledge integration can generate a new heating cup knowledge from existing heating and cup knowledge. Therefore, the user can obtain the knowledge for heating cup. Hence, this study aims to propose a novel knowledge integration method for product design.

현장일기-내가 해야 할 의무를 느끼며

김윤선,Kim, Yun-Seon 대한산업보건협회 1986 산업보건소식 Vol.33 No.-

Article - 산재저감을 위한 안전보건관리 대행기관의 활성화에 관한 연구

김윤선,Kim, Yun-Seon 한국건설안전협회 2011 建設 安全技術 Vol.54 No.-

본 연구는 산업현장의 산업재해를 저감시키기 위한 근본적인 대책을 제시하기 위하며 수행되었으며, 우리나라 산업체에 재해 방지 기관으로 핵심적인 역할을 담당하고 있는 대행기관들을 중점 조사하였다. 그래서 각 대행기관들이 안고 있는 문제점들을 파악하여 대행기관 및 대행사업장에 대한 안전상의 문제점들을 제시하며 한편으로는 대행을 받고 있는 사업장에 대한 유용한 시책들은 더욱 권장하여 중소사업장의 산재예방 전도기관으로 거듭날 수 있도록 한다. 그래서 대행기관에 활성화를 위한 실질적인 대안을 제시하고자 하는 것이 본 연구의 주요 목적이다. First of all, this paper investigated scrupulously Occupational Safety and Healthy Agencies which take care of nucleus roles as accident protection organ of industrial circles in our country. Catching hold of elaborately lots of problems that each Agency holds, we tried to action to correct that faulty custom and invalidism. On the other hand, we exhort and encourage more available measures helping practically to protect industrial accident of work place receiving vicarious execution. Furthermore, both in name and reality, we try to be reborn as a mission institution of accidental prevention of medium and small work place. Therefore, the purpose of the present paper is to suggest substantial counterproposal for activation of Occupational Safety and Healthy Agency.