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정영화(Jung Young Hwa),김익겸(Kim Ik Gyeom),김연경(Kim Yon Kyung) 강원대학교 산업기술연구소 1998 産業技術硏究 Vol.18 No.-
Service life of steel bridges depends on fatigue and corrosion. Fatigue damage result from stress concentration and initial defects in the joint of secondary member such as weldments. The objective of this study is to estimate fatigue strength of the cover plate weldments in the plate girder. For this study, fatigue tests, static tests and finete element method has been performed respectively from these results. It was found that our test results were well agreed with other test results and satisfied with the fatigue criterion of AASHTO, JSSC and EUROCODE specifications.
인공피부모델에서 지방조직 유래 중간엽 줄기세포가 표피와 기저막 형성에 미치는 효과
변희진 ( Hee Jin Byun ),이승호 ( Seung Ho Lee ),김지은 ( Ji Eun Kim ),김연경 ( Yon Kyung Kim ),조광현 ( Kwang Hyun Cho ) 대한피부과학회 2008 대한피부과학회지 Vol.46 No.9
Background: There is an increasing need for making a more ideal artificial skin model. Objective: To evaluate the effects of adipose tissue-derived mesenchymal stem cells (ATMSC) on the formation of epidermis and basement membrane in artificial skin models. Methods: ATMSC were isolated from lipo-aspirated fat tissues, and their phenotypes were confirmed by cell surface markers. Three kinds of artificial skin models were made using three different dermal substitutes. The dermal substitutes in the three models contained fibroblasts only, fibroblasts together with ATMSC or ATMSC only. The formation of epidermis and basement membrane was evaluated by immunohistochemical stains and transmission electron microscopy. Results: Among the three models, the model with both fibroblasts and ATMSC in the dermal substitute showed the most excellent formation of epidermis and, especially, basement membrane. In this model, the basement membrane proteins, laminin and type IV collagen, were expressed most apparently at the dermo-epidermal junction and, lamina lucida, lamina densa and anchoring fibrils were most evidently observed under transmission electron microscopy. Whereas, the model with only ATMSC did not show keratin 1 expression, suggesting that the ``skin-type`` stratified squamous epithelium was not formed well. Conclusion: ATMSC together with fibroblasts can be used effectively in constructing artificial skin models. (Korean J Dermatol 2008;46(9):1186~1193)