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대학 환경교육에서 윤리적 성찰과 공감의 필요성에 관한 연구
김양지(Kim, Yang Jee),홍경남(Hong, Kyung Nam) 중앙대학교 중앙철학연구소 2016 철학탐구 Vol.41 No.-
이 논문에서는 대학 환경교육에서 윤리적 성찰과 공감의 측면이 강조되어야 한다고 논증하겠다. 환경문제에서 윤리적인 측면의 근본 물음들을 제기하고 그에 답변하는 과정은 중요하다. 이런 과정은 바로 환경에 대한 윤리적 태도 변화를 위한 가장 강력한 유인 역할을 할 수 있다. 적어도 대학 수준에서 ‘이유’를 이해한다는 것 이상의 동기유발 요소는 없기 때문이다. 그 취약성에 관해 공감하면서 환경에 대한 윤리적 성찰을 이뤄내기 위해서 환경교육에 환경 텍스트를 적극적으로 활용하는 방안을 모색한다. 그리고 이런 과정에서 비판적 사고 능력 및 공감 내지 감수성 능력이 함양되고, 이는 환경 친화적인 태도 변화로 이어질 수 있다고 주장할 것이다. This paper relates to the cultivation of ethical deliberation and sympathy in the university environmental education practice. It is very important to make ethically fundamental questions and answer them. This question and answer process might be, to university students, an incredibly powerful catalyst to change in attitude toward environments. In order to ethically deliberate on the significance of the environments to humans while being sympathetic to their weakness, it is attempted to positively utilize environmental texts in the environmental education. And in this way, the students exert or cultivate the capacities of critical thinking and sympathy so that their lives could change in an environment-friendly manner.
DNA 회복 저해제 Cytosine Arabinoside, 3-Aminobenzamide 및 Hydroxyurea가 방사선에 의해 유도된 소핵과 이수성에 미치는 영향
조윤희,김양지,강창모,하성환,정해원,Cho, Yoon-Hee,Kim, Yang-Jee,Kang, Chang-Mo,Ha, Sung-Whan,Chung, Hai-Won 대한방사선방어학회 2005 방사선방어학회지 Vol.30 No.4
소핵분석은 방사선의 생물학적 선량계로서 활용되고 있으나 이의 생성 기전은 아직까지 확실치 않다. 본 연구에서는 사람 림프구에 방사선을 조사한 후 DNA 손상회복 저해물질, Cytosine Arabinoside(Ara C)와 3-Aminobenzamide(3-AB) 그리고 Hydroxyurea(HU)를 특정 세포주기에 처리하고 소핵분석과 FISH기법을 이용하여 방사선에 의한 소핵 및 이수성의 정도를 구명하고자 하였다. 방사선 선량에 따라 소핵과 이수성의 빈도는 양반응 관계를 보이며 증가하였고 DNA 손상회복 저해물질 처리 후 소핵의 빈도는 모든 DNA 손상 회복 저해물질에 의해 증가하였으며 Ara C, 3AB, HU 순으로 나타났다. 이수성의 빈도는 HU와 Ara C에 의해서 크게 증가하였으나, 3AB는 아무런 영향을 주지 않았다. 또한 1번 염색체가 4번 염색체보다 방사선에 의한 소핵형성 및 이수성에 더 많이 관여되었다. 본 연구 결과, 방사선에 의한 소핵 및 이수성의 형성 과정은 여러 다른 기전이 관여하고 있음을 알 수 있었다. This study was carried out to examine the effect of the DNA repair inhibitors, Cytosine Arabinoside(Ara C), 3-Aminobenzamide(3AB) and Hydroxyurea(HU) on the frequencies of radiation-induced micronuclei(MNi) and aneuploidy. Irradiated lymphocytes(1-3Gy) were treated with DNA repair inhibitors, Ara C, 3AB and HU for 3 hours and CBMN assay - FISH technique with DNA probe for chromosome 1 and 4 was performed. The frequencies of x-ray induced MNi and aneuploidy of chromosome 1 and 4 were increased in a dose-dependent manner. Ara C, 3AB and HU enhanced the frequencies of radiation-induced MNi and the frequencies of radiation-induced aneuploidy of chromosome 1 and 4 were enhanced by HU and Ara C while no effect was observed by 3AB. The frequency of radiation-induced aneuploidy of chromosome 1 was higher than that of chromosome 4. These results suggest that there are different mechanisms involved in the formation of MNi and aneuploidy by radiation.
에틸렌옥사이드(Ethylene oxide)에 노출된 병원 근로자들의 소핵 빈도와 유전적 감수성 지표와의 연관성
이선영,김양지,최영주,이중원,이영현,신미연,김원,윤충식,김성균,정해원,Lee, Sun-Yeong,Kim, Yang-Jee,Choi, Young-Joo,Lee, Joong-Won,Lee, Young-Hyun,Shin, Mi-Yeon,Kim, Won,Yoon, Chung-Sik,Kim, Sung-Kyoon,Chung, Hai-Won 한국환경보건학회 2011 한국환경보건학회지 Vol.37 No.6
Objectives: Ethylene oxide (EtO) is classified as a human carcinogen, but EtO is still widely used to sterilize heat-sensitive materials in hospitals. Employees working around sterilizers are exposed to EtO after sterilization. The aim of the present study was to assess the exposure of EtO level, coupled with occupationally induced micronuclei from hospital workers. The influence of genetic polymorphisms of detoxifying genes (GSTT1 and GSTM1) and DNA repair genes (XRCC1 and XRCC3) on the frequencies of micronuclei in relation to exposure of EtO was also investigated. Methods: The study population was composed of 35 occupationally exposed workers to EtO, 18 student controls and 44 unexposed hospital controls in Korea. Exposure to EtO is measured by passive personal samplers. We analyzed the frequencies of micronuclei by performing cytokinesis-block micronucleus assay (CBMN assay) and GSTM1, GSTT1, XRCC1, and XRCC3 were also genotyped by performing polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). Results: The frequencies of micronuclei in EtO exposure group, student controls and hospital controls were $18.00{\pm}7.73$, $10.47{\pm}7.96$ and $13.86{\pm}6.35$ respectively and their differences were statistically significant, but no significant differences according to the level of EtO were observed. There was a dose-response relationship between the frequencies of micronuclei and cumulative dose of EtO, but no significantly differences were observed. We also investigated the influence of genetic polymorphisms (GSTM1, GSTT1, XRCC1, and XRCC3) on the frequencies of micronuclei, but there were no differences in the frequencies of micronuclei by genetic polymorphisms. Conclusions: The frequencies of micronuclei in EtO exposure group was significantly higher than control groups. A dose-response relationship was found between the level of EtO exposure and the frequencies of micronuclei, but no statistically differences were observed. We also found that the frequencies of micronuclei were increased according to cumulative EtO level. There was no association of the genetic GSTM1, GSTT1, XRCC1, and XRCC3 state with the frequency of micronuclei induced by EtO exposure.
한국인에서의 DNA repair gene(hMLH, hMSH2 및 ATM)의 Single Nucleotide Polymorphisms(SNPs)의 빈도
정현숙(Hyun Sook Jung),김태연(Tae Yon Kim),조윤희(Yoon Hee Cho),김양지(Yang Jee Kim),정해원(Hai Won Chung) 한국환경성돌연변이발암원학회 2003 한국환경성돌연변이·발암원학회지 Vol.23 No.1
Single nucleotide polymorphisms (SNPs) are alterations in DNA base that occur most frequently throughout the human genome. The SNPs of DNA repair genes, hMLH1, hMSH2 and ATM, among 100 Korean people were analyzed using Dynamic Allele specific Hybridization (DASH) techniques. Mutation at the position of exon 38 (GA) and exon 10 (CG) of ATM gene, mutation at the position of exon 8 (AG), and exon 1 (AG) of hMLH1 gene and exon 14 (AG) of hMSH2 gene were investigated. No mutation at the selected position of ATM gene and hMSH1 gene was found . However, while there was no mutation at the position of exon of hMSH2 gene, mutation was found at the promotion region (CT) with the frequency of 24% CC, 36% CT and 62% TT genotyes. This results might be used as baseline data for research on SNP of Korean population.
Di-2-EthylHexyl Phthalate, 2-EthylHexanoic Acid 및 Di-2-Ethyl Phthalate의 유전독성 평가
송주영(Joo Young Song),조윤희(Yoon Hee Cho),김양지(Yang Jee Kim),정해원(Hai Won Chung) 한국환경성돌연변이발암원학회 2005 한국환경성돌연변이·발암원학회지 Vol.25 No.3
DEHP is one of well known endocrine disruptor and it is used as additives for the production of PVC. There has been contradictional result on the genotoxicity of DEHP. In order to examine genotoxicity of a endocrine disruptors, DEHP (Di-2-EthylHexyl Phthalate) and it's two metabolites, EHA (2-EthylHexanoic Acid) and DEP (Di-2-Ethyl Phthalate), chromosome aberration (CA), sister chromatid exchange (SCE), micronuclei (MN) and single cell gel electrophoresis were analysised. No increase of the frequency of CA was observed by DEHP and its two metabolites. DEHP increased the frequency of SCE and MN whereas EHA only increased the frequency of SCE. DEP increased the frequency of SCE but the increase was not statistically significant. DEHP and DEP, also induced DNA damage. It is suggested that combination of different methods were recomended to find the genotoxicity of DEHP and its metabolites.
극 저주파(60㎐)의 전자장이 동물세포에 미치는 세포 유전학적 효과
정해원,김양지 서울大學校保健大學院 1992 國民保健硏究所硏究論叢 Vol.2 No.2
The effect of extremely low frequency(60Hz) electromagnetic field radiation on human perpheral lymphocytes and CHO cells was studied as to the induction of chromosome aberration, sister chromatid exchange and point mutation at HGPRT locus. Human lymphocytes and CHO cells exposed to 30kV/m, 60Hz electric field for 20min, 1hr, and 2hrs showed no increase of chromosome aberration and sister chromatid exchange. CHO cells exposed to electric field for 1hr and 2hrs showed no increase of mutation frequency, but plating effciencies were decreased. When mice were chronically exposed to 6kV/m, 60Hz electric field for 1hr per day up to 12weeks, no increase of chromosome aberration in bone marrow cells was observed. It was concluded that there was no obvious induction of cytogenetic damages by ELF radiation at the conditions in this experiment.
사람 림프구에서 BLEOMYCIN 및 MITOMYCIN - C 에 의한 적응 반응
정해원,김양지 한국유전학회 1992 Genes & Genomics Vol.14 No.3
Human peripheral blood lymphocytes pre-exposed to low dose of Bleomycin which can induce double strand breaks in DNA, became less susceptible to the induction of chromatid breaks by high dose of same agent or by Mitomycin C which can induce cross-links in DNA, than those which were not pre-exposed to Bleomycin. But although lymphocytes adapted to Mitomycin C showed resistance to high concentration of the same agent, they did not show cross resistance to high concentration of Bleomycin. These results indicate that there might be different repair mechanisms in the adaptive response induced by different DNA damaging agents.