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Propionylthiocholine-Dithiobis 방법에 의한 사람과 몇몇 동물 혈청내의 Cholinesterase 활성도 측정
김순태,김성훈,도재철,이영호,모기철,허린수,Kim, Soon-tae,Kim, Sung-hoon,Do, Jae-cheul,Lee, Young-ho,Mo, Ki-cheul,Huh, Rhin-sou 대한수의학회 1987 大韓獸醫學會誌 Vol.27 No.1
Determination of cholinesterase activity is a routine practice in many laboratories to detect the influence of cholinesterase inhibiting drugs such as organophosphate and carbamate insecticides. Among many different methods to determine the cholinesterase activity, the present method was the most recent, simple and accurate one for routine test in clinics. The results obtained in sera of human and the different species of animals by means of the present method were as follows: $5.76{\pm}1.12U/ml$ in human, $3.37{\pm}0.83U/ml$ in german shepherd, $0.61{\pm}0.18U/ml$ in rat, $14.91{\pm}3.10U/ml$ in mouse, $1.55{\pm}0.51U/ml$ in chicken, $0.28{\pm}0.11U/ml$ in slaughtered cattle and $0.50{\pm}0.10U/ml$ in slaughtered pig.
BB 랫드 및 streptozotocin이 투여된 랫드에서 vitamin E와 insulin 병합 투여 영향 I. 지질과 단백질의 산화성 손상 및 혈청내 효소 활성도에 미치는 영향
김순태,허린수,Kim, Soon-tae,Huh, Rhin-sou 대한수의학회 1995 大韓獸醫學會誌 Vol.35 No.4
The present study, to evaluate the effect of vitamin E on the oxidative stress in STZ-treated rat and BB rat, was investigated the biochemical enzyme activity in the serum, and malondialdehyde and carbonyl group in the RBC membrane, liver and microsomal fraction after vitamin E and/ or insulin treatment. Results obtained through the experiments were summarized as follows; 1. Effect of vitamin E and/or insulin treatment in STZ-treated rat 1) Lipid peroxidation level in RBC membrane, liver and microsomal fraction was significantly decreased in vi. tamin E and/or insulin treatment group, and especially more significantly decreased in vitamin E with insulin treated group. 2) Protein oxidation level in RBC membrane, liver and microsomal fraction was significantly decreased in vitamin E and/or insulin treatment group. And it was especially more significantly decreased in RBC membrane and liver of vitamin E with insulin treated group. 3) In the enzyme activity in the serum, the activity of AST and ALT was not altered in all experimental group. The increased ALP activity in STZ-treated group was significantly decreased in insulin treated group and vitamin E with insulin treated group. 4) Decreased level of albumin and creatinine after STZ treatment was significantly increased in vitamin E and/or insulin treated group. 5) Level of glucose, cholesterol and triacylglycerol in serum: Glucose level was not significantly different in vitamin E treated group compared to STZ control group. But it was significantly different in the insulin treated group and vitamin E with insulin treated group compared to STZ control group. The cholesterol content in the serum was significantly increased in STZ control group compared to normal control group. And except low dose vitamin E treatment group, it was significantly decreased in vitamin E and/or insulin treated group compared to STZ control group. The triacylglycerol content in the serum was significantly decreased in STZ control group and increased in high dose vitamin E treated group and vitamin E with insulin treated group. But it was not significantly different in low dose vitamin E treated group and insulin treated group compared to STZ control group. 2. Effect of vitamin E and/or insulin treatment in BB rat 1) Lipid peroxidation level in liver was decreased by vitamin E with insulin treatment compared to insulin treatment. But it was not different in microsomal fractions. 2) Protein oxidation level in liver and microsomal fraction was decreased by vitamin E with insulin treatment compared to insulin treatment only in microsomal fractions. These results suggest that the combination treatment of vitamin E and insulin could prevent the oxidative change of lipid and protein of the RBC membrane, liver and microsomal fraction in STZ-treated rats and BB rats.
칼럼 - 미세먼지 해결을 위한 선(先) 이해와 후(後) 시행
김순태,Kim, Sun-Tae 대한석유협회 2018 석유와 에너지 Vol.2018 No.-
국내에서 대기 중에 존재하는 미세먼지 ($PM_{10}$, $PM_{2.5}$) 농도는 법적 허용치인 대기환경기준을 현재 초과하고 있으며, 2018년 3월말 강화된 기준을 달성하기 위해서는 어쩌면 우리가 '상상'할 수 있는 모든 방안을 이끌어내고 적극적으로 시행하는 방법 외에는 다른 선택의 여지가 없을 지도 모른다. 참으로 어려운 문제이다. 환경부의 2017년 대기환경연보를 보면 2016년 서울의 $PM_{2.5}$ 농도는 $26{\mu}g/m^3$으로 새로운 기준인 $15{\mu}g/m^3$를 달성하기 위해서는 대략 $11{\mu}g/m^3$ 만큼의 미세먼지 농도 감소가 필요하다. 중국 등 국외 배출량의 영향이 감소하고는 있다지만, 아직은 그 영향이 작지 않다. 많은 국민들이 이미 인지하고 있듯이, 국외 영향이 절반을 차지한다고 가정하면, 우리는 우리가 배출하는 모든 것을 중지시켜야 목표 농도 달성이 가능할 정도다. 과연 그 누가 그러자고 선 듯 나서서 말할 수 있을까?
BB 랫드 및 streptozotocin이 투여된 랫드에서 vitamin E와 insulin 병합 투여 영향 II. 인지질의 지방산 조성에 미치는 영향
김순태,허린수,Kim, Soon-tae,Huh, Rhin-sou 대한수의학회 1995 大韓獸醫學會誌 Vol.35 No.4
The present study was investigated fatty acid composition of the phospholipid in the RBC membrane, liver and microsomal fraction after vitamin E and/or insulin treatment to evaluate the effect of vitamin E on the oxidative stress in STZ-treated rat and BB rat. Results obtained through the experiments were summarized as follows; 1. Effect of vitamin E and/or insulin treatment in STZ-treated rat 1) In the insulin treated group and the combination treated groups of vitamin E with insulin, body weights were increased compared to STZ-treated rat(STZ control group). Especially it was more significantly increased in the combination treated group of high dose vitamin E with insulin. 2) The composition of fatty acids of the phospholipid in RBC membrane, liver and microsomal fractions was shown a decreased C16:1, C18:1, C20:4 and an increased C16:0, C18:0, C18:2 in STZ control group compared to normal control group. In RBC membrane, liver and microsomal fractions after vitamin E with insulin treatment in STZ-treated rat, effect on the composition of fatty acids of the phospholipid was shown the result of a decreased C16:0, C18:0, C18:2 and an increased C16:1, C18:1, C20:4. 3) Hemolysis rate of the RBC to $H_2O_2$ was increased in the STZ control group and it was decreased below the hemolysis level of normal control group by vitamin E treatment. 2. Effect of vitamin E and/or insulin treatment in BB rat 4) Only in microsomal fraction, fatty acid composition was different between insulin treatment group and vitamin E with insulin treatment group. It was increased C16:0 and C18:1, and decreased C18:0 and C18:2 in vitamin E with insulin treatment group: But C20:4 was not different in two groups. These results suggest that the combination treatment of vitamin E and insulin could prevent the oxidative change of fatty acids in P-lipid of the RBC membrane, liver and microsomal fraction in STZ-treated rats and BB rats.
경북지역 산란계에서 avian pneumovirus에 대한 항체가 조사
김순태 ( Soon Tae Kim ),김성국 ( Sung Kuk Kim ),조민희 ( Min Hee Cho ),김영환 ( Young Hoan Kim ) 한국가축위생학회 2003 韓國家畜衛生學會誌 Vol.26 No.1
Avian pneumovirus(APV), also known as avian rhinotracheitis virus(ARTV), affects both turkeys and chickens and is known to be the primary causative agent of turkey rhinotracheitis (TRT). The aim of this study was to establish the presence or absence of antibodies to avian pneumovirus in the commercial poultry population of Korea For this purpose, chicken serum samples were obtained and tested by an enzyme-linked immunosorbent assay(ELISA). The tested serum was collected in laying hens with reduction of egg production or normal in Gyeongbuk province. A total of 184 sera representing 42 different poultry farms of the Gyeongbuk region of Korea were included in this study. Laying hens of 16 different farms with reduction of egg production and laying hens of 26 different farms with clinically healthy at the time of serum sampling were considered positive to antibody against APV. In the farms with reduction of egg production, positive farm to antibody against avian pneumovirus were 11 of 16 different farms(68.8%) and positive sera were 47(58.8%) of 80 different serum. In the farms with clinically healthy flock, positive farm to antibody against avian pneumovirus were 12(46.2%) of 26 different farms and positive serum sample were 39(37.5%) of 104 different sera. According to the results tested to 42 different farms in 14 city, 8 of 14 city have flocks with antibody positive laying hens against APV, 1 of 14 city have antibody suspicious and 5 of 14 city shown antibody negative, respectively.
뉴캣슬병 생독백신 접종 후 야외 분리 바이러스에 대한 면역성 조사
김순태 ( Soon Tae Kim ),박인화 ( In Hwa Park ),김성국 ( Sung Kook Kim ),김영환 ( Young Hoan Kim ),조광현 ( Kwang Hyun Cho ),손재권 ( Jae Kwon Son ) 한국가축위생학회 2001 韓國家畜衛生學會誌 Vol.24 No.2
This study was conducted to determine vaccination programs for the control of Newcastle Disease (ND) in chickens and investigate protective effect against Newcastle disease virus(NDV) after live ND vaccination. Maternal HI antibody titer level of chickens according to day(age) 1, 7, 14, 21, 28 and 35 were decreased gradually as 7.10±0.74, 6.57±1.27, 3.71±1.25, 2.20±1.03, 1.20±1.23 and 0.50±0.71. As a result of HI test and ELISA, both chickens vaccinated with VG/GA strain live vaccine at 1-day-old and chickens not vaccinated do not have antibody titer for protection against NDV at 14-day-old. Except for LaSota strain vaccine, in case of vaccination with VG/GA spray and VG/GA, B1 and LaSota strain drinking water at 14-day-old, the protective effect was 100% in chickens inoculated NDV(10(7.2) EID50/50㎕, eye drop) at 21-day-old, but not 10~50% at 28-day-old. These data suggest that live NDV vaccination should be given at 10-day-old and 20-25 day-old for protect against NDV at periodic outbreaks of ND caused by velogenic viscerotropic NDV in the environment of a farm.