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교류 유전영동 및 횡단면 전열유동 기반의 선택적인 박테리아 채집을 위한 미세유체 장치
한창호(Chang-Ho Han),김명우(Myeong-Woo Kim),김기훈(Gi Hoon Kim),김동민(Dongmin Kim),박재형(Jae Hyeong Park),하준엽(Jun Yeop Ha),장재성(Jaesung Jang) 대한기계학회 2016 대한기계학회 춘추학술대회 Vol.2016 No.12
Dielectrophoresis (DEP) is an effective technique for manipulating a specific species of particle from mixtures when they are applied to microfluidic devices, and this can be used for many analyses like disease diagnosis, food hygiene, or microorganism monitoring. Planar electrodes, such as interdigitated electrodes, are widely used to induce DEP in microfluidic devices because of its simple fabrication processes, but as the DEP needs high spatial non-uniform electric fields, the particles which are distant from the electrodes are not manipulated properly. So, for the enough particle manipulation by DEP in microfluidic channel with keeping using the planar electrodes, AC electrothermal (ET) flow were used to assist those works. On the main channel flow whose direction is longitudinal, the electrothermal flow were intended to be induced in cross-sectional direction of the microchannel to induce the flow continuously. To operate the DEP and ET flow simultaneously using AC signals, the planar electrodes were designed properly, and at 1 MHz of AC frequency, the target bacteria Escherichia coli (E. coli) can be captured by positive DEP on to the electrodes, and ET flow can be induced as well. So, many of distant bacteria can be pulled to the electrodes by ET flow, and captured by positive DEP. In a microchannel of 300 um width and 160 um height, around 60% of bacteria coming into the microfluidic device can be selectively captured even many of them were located further from the electrodes.