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동해안 개다시마 Kjellmaniella crassifolia Miyabe (Phaeophyta)의 초기배양과 생장특성 연구
김영대 ( Young Dae Kim ),송홍인 ( Hong In Song ),김진희 ( Jin Hee Kim ),홍정표 ( Jung Pyo Hong ),전창영 ( Chang Yeong Jeon ),한형균 ( Hyoung Kyun Han ),김수경 ( Su Kyoung Kim ),김동삼 ( Dong Sam Kim ),김명래 ( Myoung Rae Kim ) 한국수산과학회 2007 한국수산과학회지 Vol.40 No.5
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Plasminogen kringle 5 재조합 단백질에 의한 ERK1/2 활성화 및 세포골격 재배열 억제
하정민,김현경,김명래,조영애,Ha, Jung-Min,Kim, Hyun-Kyung,Kim, Myoung-Rae,Joe, Young-Ae 한국생명과학회 2006 생명과학회지 Vol.16 No.7
Plasminogen kringle 5는 plasminogen kringles 1-4로 구성된 내생의 혈관 신생 억제제인 angiostatin과 같이 내피세포의 분열을 강력하게 억제한다고 알려져 있다. 본 연구에서는 plasminogen kringle 5의 재조합 단백질을 효모 발현 체계에서 생산하여 내피세포의 이동에 대한 저해 효과와 이에 대한 작용기전을 조사하였다 재조합 단백질 PK5는 plasminogen의 Thr456에서 Phe546까지 이르는 cDNA 부분을 ${\alpha}-factor$ prepro-peptide의 분비 신호 서열 뒤에 도입하여 Pichia pastoris GS115에서 발현시켰다. 메탄올 유도 후 얻은 배양액을 S-spin column을 이용하여 정제하였다. 정제된 단백질을 SDS-PACE하였을 때 약 10kDa의 단일 밴드를 나타냄을 확인할 수 있었다. 정제된 PK5는 bFGF나 VEGF에 의해 유도된 인간의 제대 유래 내피 세포의 이동을 약 500nM의 $IC_{50}$ 값으로 농도 의존적으로 감소시켰다. 내피 세포에 PK5 500M을 처리한 결과 bFGF에 의해 유도된 ERK1/2의 인산화를 감소시켰다 또한, PK5는 bFGF에 의해 유도된 내피세포의 골격 재형성을 강력하게 억제하는 것으로 관찰되었다. 따라서, 이러한 결과들은 효모 생산 PK5가 내피세포의 이동을 효과적으로 억제하며, 이는 ERK1/2의 활성과 세포골격의 재배열을 억제함으로써 나타나는 것으로 부분적으로 설명될 수 있다. Plasminogen kringle 5 is a potent inhibitor of endothelial tell proliferation like an endogenous angiogenesis inhibitor, angiostatin consisting of plasminogen kringles 1-4. In this study, we produced the recombinant protein of plasminogen kringle 5 (PK5) employing an Pichia expression system and examined its. effect on~endothelial cell migration and its possible inhibitory mechanism. PK5 was expressed in Pichia pastoris GS115 by fusion of the cDNA spanning from Thr456 to Phe546 to the secretion signal sequence of a-factor prepro-peptide. After methanol induction, the secreted PK5 was purified by using S-spin column. SDS-PACE analysis of the purified protein showed one major band of approximately 10kDa. In in vitro migration assays, the purified protein inhibited dose-dependently the migration of human umbilical endothelial cells (HUVECs) induced by basic fibroblast growth factor (bFGF) or vascular endothelial growth factor (VEGF) with an $IC_{50}$ of approximately 500nM. Accordingly, it inhibited bfGF-stimulated extracellular signal-regulated kinase 1/2 (ERK1/2) phosphorylation in HUVECs at 500nM. In addition, it also potently inhibited bFGF-induced cytoskeletal rearrangement of HUVECs. Thus, these results suggest that Pichia-produced PK5 effectively inhibits endothelial cell migration, in part by suppression of ERK1/2 activation and blocking cytoskeleton rearrangement.
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김태규(Tae Kyu Kim),김명래(Myoung Rae Kim) 대한악안면성형재건외과학회 1981 Maxillofacial Plastic Reconstructive Surgery Vol.3 No.1
This is to report a case of surgical correction for extreme micrognathia combined with TMJ The 30 years old male patient had sufferred from masticatory inability due to locked jaw since child hood after febrile arthritic illness on Rt. temporomandibular joint area, and facial disfigurement for asymmetric bird-face had been induced d end distinguished by his growing. After precise examination &, analysis the patient was treated by a serial approaches of unilateral condylectomy, physical exercise and mandibular augumentation as follows; 1. Rt. mandibular condyle being united fibrously was resected oy preauricular approach, and fascial interpositioning was performed to prevent reunion of the bony segments. 2. Early and active exercise to open the jaw had been rendered for 4 months. 3. The bilateral step sliding osteotomy lengthened the mandibular body and the grafted iliac bones were maintained in the inferior space by interosseous wiring and intermaxillary fixation for 6 weeks. In reviewing this case following points were noted. l. If the ease is unitaterai ankylosis, arthroplastic implant after condylectomy my not be utilized to prevent shifting and open bite. 2. Resection of proximal con4ylar segment more than 10 mm in width was not fallowed by recurrence of ankylosis without postoperative bite block to create intersegmental apace. 3. Active exercise soon after the operation being lasted until the second surgery were of value to improve and maintain the jaw excursion movement. 4. Step sliding augumentation of the mandibular body as much as 10 mm in length required bone graft at inferior defect, however, didn`t show any neuropathy nor vascular collapse. 5. To obtain better cosmetic result, chin plasty may be considered as additional augumentation & contouring.
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상악 구치부에서 자가골편 이식술의 예후와 골 변화량에 관한 후향적 연구
명미랑(Mee-Rang Myoung),김명래(Myung-Rae Kim),김선종(Sun-Jong Kim) 대한구강악안면외과학회 2009 대한구강악안면외과학회지 Vol.35 No.5
Purpose: The purpose of this study was to evaluate the surgical success of bone reconstruction of the severely atrophic maxilla using autogenous block bone onlay graft from the ramus and ilium prior to dental implantation. And we measured the amount of vertical height change Material and Methods: 26 partially edentulous patients(32 case) who needed block onlay bone graft before implant placement in posterior maxillary area from 2002 to 2009 were selected for this study. Patients consisted of 20 males & 6 females and the average of their age was 54.2. Patients who were treated with ramal bone were 19 case and patients who were treated with iliac bone were 11 case. Digital panoramic X-ray was taken at the day of surgery, 3 months and 6 months later after the surgery. Vertical height change & resorption rate of grafted bone were measured with the same X-rays and compared Results: Two out of 32 bone grafts had to be removed because of inflamation at the grafts area(97.3%). The mean of radiographic vertical height change(change rate) of post-op. 3 month was 0.54mm(8.5%)and 6 month was 0.99mm(15.9%). Compairing to intraoral donor site(ramus), iliac bone had more vertical height change(1.18mm) at 6 month after surgery. Conclusions: Within the limit of this study, autogenous block onlay grafts can be considered a promising treatment for severely atrophic maxilla
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