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Physiological Evaluation of Transgenic Rice Developed for Drought Tolerance
김길웅,Sita Ram Ghimire,Sang-Kyu Park,강동진,이인중,Dong-Hyun Shin,Sung-Uk Kim 한국식물생명공학회 2006 JOURNAL OF PLANT BIOTECHNOLOGY Vol.33 No.2
Evaluation of physiological performance of trehalose- producing transgenic rice line was conducted to investigate drought tolerance at early growth stage. Under artificially induced drought condition of 8% polyethylene glycol 6000, this transgenic rice line had leaf photosynthetic rate of 11.08 μmol CO2 m-2s-1, leaf transpiration rate of 8.38 mmol H2O m-2s-1 and leaf water potential of -1.12 MPa after 96 hours of treatment. Nakdongbyeo, the parent of this transgenic rice line, had photosynthetic rate of 15.42 μmol CO2 m-2s-1, leaf transpiration rate of 8.04 m㏖ H2O m-2s-1 and leaf water potential of -0.88 MPa. The other variety used in this experiment for comparison, IR 72, showed higher values than both transgenic rice line and variety Nakdonbyeo on all three parameters; leaf photosynthetic rate of 20.61 μmol CO2 m-2s-1, leaf transpiration rate of 12.88 mmol H2O m-2s-1, and leaf water potential of -0.82 MPa. So this transgenic rice line did not show superior performance in leaf transpiration rate, leaf photosynthetic rate and leaf water potential compared to variety Nakdongbyeo. This result along with visual observation on leaf rolling and drying during the experimental period indicated poor physiological performance of this transgenic rice line. Further studies on metabolic status of stress-induced trehalose, along with study on physiological response of this transgenic rice line during drought stress would shed more light on overall physiological performance of this transgenic rice line.
김길웅,박용구,최명석,Kim, K.U.,Park, Y.G.,Choi, M.S. 한국잡초학회 1995 Weed&Turfgrass Science Vol.15 No.2
Anthocyanin formation in callus cultures using Populus alba ${\times}$ Populus glandulosa was evaluated on basal MS medium supplemented with various levels of growth regulators, sucrose and nitrate concentrations. The highest yield of anthocyanin from cultured cells was produced under 5% sucrose, 1/8 strength of nitrate(12.5% of basic concentration) and combination of 1.0 mg/l IAA with 2 mg/l BAP, respectively. The high anthocyanin producing cell line no. 11 was selected among 15 cell lines, showing over 80% cells contained anthocyanin producing cells. From these cells, the highly productive red protoplast was isolated and the highest protoplast yield, $6.7{\times}10^6$ was obtained in enzyme combination IV which is composed of 2.0% cellulase, 0.5% macerozyme and 0.1% pectolyase. 현사시나무의 조직 배양 세포에서의 anthocyanin 생산성이 높은 세포계를 얻기 위한 목적으로 실험을 수행한 바 다음과 같은 결과를 얻었다. Anthocyanin 생산력이 높은 세포계의 선발은 sucrose 3%, 2, 4-D 0.5 mg/l, BAP 0.1 mg/l가 첨가된 MS 기본배치에서 실시하여 15 개 cell line중에 생성력이 80%에 달한 ACL 11 세포계를 선발하였다. Anthocyanin 생성에는 배지 환경요소가 중요한 인자로 작용함을 밝혀 냈다.