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조직배양한 Vero E6 세포에서의 한탄바이러스 및 서울바이러스 분리
김금철 고려대학교 의과대학 1987 고려대 의대 잡지 Vol.24 No.1
The etiological agent of Hemorrinagic fever with renal syndrome (HFRS) had not been succeeded to isolate for more than 6 decades since its first description in Russia in 1913. For the first time by Lee et al., the causative antigen was isolated from lung tissue of Apodemus agrarius in Korea in 1976 by immunofluorescent antibody technique and named it as Hantaan virus in 1980. Lung section from Apodemus mice infected with Hantaan virus was utilized as antigen in serological diagnostic test of HFRS but lung tissues from the mice might have the problems of contamination with reovirus and other respiratory viruses. A successful gorwth of the virus in an in vitro system, a human cell line designated A549 was first reported by French et al. in 1981. We have propagated Hantaan virus in Vero E6 cells, and studied antigenicity, multiplication and antibody response in various animals after intramuscular inoculation of the virus cultured in Vero E6 cells. The summary of this study were as follows: 1. Hantaan virus and Seoul virus were isolated in Vero E6 cells and well multiplied. 2. Hantaan virus isolated from lung tissues of infected Apodemus mice and patient's blood were not contaminated with reovirus, which was studied by immunofluorescent antibody technique and antibody response of S.D. rats. 3. After intramuscular inoculation of Hantaan virus cultured in A549 cells and Vero E6 cells into Apodemus mice and rats, multiplication of the virus was demonstrated in the lung tissues in partial numbers of Apodemus mice but not in rats and antiboy titers were revealed higher in rats than in Apodemus mice.
[차량운동성능] 상용차용 공기스프링 현가장치에 관한 연구
탁태오,김금철 한국자동차공학회 1999 한국자동차공학회 춘 추계 학술대회 논문집 Vol.1999 No.11_2
This paper presents a method for evaluating the performance of a leaf spring suspension and an air spring suspension systems for trucks in terms of ride and handling. Leaf springs which generally have non-linear progressive force-deflection characteristics, are modeled using beam and contact elements. The leaf spring model shows good correlation with experimental data. Each component of an air spring suspension system. which is a single leaf, air spring, height control valve compressor and linkages is modeled appropriately. Non-linear characteristics of air spring are accounted for using the measured data, and pressure and volume relations for height control system is also considered. The wheel rate of the air suspension is taken lower but roll stiffness is taken higher than those of leaf springs to improve ride and handling performance, which is verified through driving simulations
탁태오,김금철,Tak, tae-oh,Kim, kum-Chul 강원대학교 산업기술연구소 1999 産業技術硏究 Vol.19 No.-
This paper presents a method for evaluating the performance of a leaf spring suspension and an air spring suspension systems for trucks in terms of ride and handling. Leaf springs, which generally have non-linear progressive force-deflection characteristics, are modeled using beam and contact elements. The leaf spring analysis model shows good correlation with experimental results. Each component of an air spring suspension system, which is a single leaf, air spring, height control valve, compressor and linkages, is modeled appropriately. Non-linear characteristics of air spring are accounted for using the measured data, and pressure and volume relations for height control system is also considered. The wheel rate of the air suspension is taken lower but roll stiffness is taken higher than those of leaf springs to improve ride and handling performance, which is verified through driving simulations.