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외기요법(外氣療法)과 영연온담탕(靈連溫膽湯) 및 적연온담탕(赤連溫膽湯)이 복수암(腹水癌) 생쥐의 조혈계통(造血系統)에 미치는 영향(影響)
길호식,김광호,Khil Ho-Sik,Kim Kwang-Ho 대한예방한의학회 1998 대한예방한의학회지 Vol.2 No.1
In order to study the effects of emitted-qi therapy, Youngyeonondamtang and Jeokyeonondamtang on the mice bearing ascites cancer caused by Sarcoma 180, the author divided the mice into normal group, control group, EQ group, YO group, JO group, CTX+QE group, CTX+YO group, CTX+JO group, and treated with emitted -qi therapy and administrated Youngyeonondamtang, Jeokyeononondaintang and Cyclophosphamide (CTX). After collecting blood from the mice, measured the values of Prothrombin Time(PT), Partial Thromboplastin Time(PTT), Fibrinogen, White Blood Cell(WBC) and Platelet. The results were as follows, 1. PT was not showed any significant change in ever)r group, being compared with the control group. 2. H increased significantly in YO group, being compared with the control group. 3. Fibrinogen decreased significantly in EQ group, YO group, CTX+EQ group, CTX+YO group, CTX+JO group, being compared with the control group. 4. WBC increased significantly in CTX+EQ group, CTX+YO group, CTX+JO group, being compared with the CTX group. 5. Platelet decreased significantly in every group, being compared with the control group. These results suggest that emitted-qi therapy, Youngyeonondamtang and Jeokyeonondamtang had the effects that could heal the hematopoiesis system in the mice bearing ascites cancer, and especially had the better effects in the case that hematopoiesis system has been impaired by CTX.
연년익수불로단(延年益壽不老丹)이 노화유발 흰쥐의 항산화능에 미치는 영향
길호식,이송실,이상재,김광호,Khil, Ho-Sik,Lee, Song-Shil,Lee, Sng-Jae,Kim, Kwang-Ho 대한예방한의학회 2002 대한예방한의학회지 Vol.6 No.2
Objectives: Younnyeniksoobulrodan(延年益壽不老丹) composed of Polygonum multiflorum THUNB. and some medical herbs is known as formula of senescence delay effect. The purpose of this study is to investigate the effect of Younnyeniksoobulrodan(延年益壽不老丹) on antioxidant enzyme activity such as Thiobarbituric acid reactive substance(TBARS), Superoxide dismutase(SOD), Catalase(CAT), Glutathione peroxidase (GSH-px) in rat erythrocytes and liver. Methods: Sprague-Dawley rats divided into 4 gorups, Young group(8 weeks old, N-8), Aging group(18 weeks old, N-18), pathologically induced aging gorup(injected D-galatose 50mg/kg, 1time/day for 6 weeks, CON) and Younnyeniksoobulrodan(延年益壽不老丹) administered group(D-galactose 50mg/kg and Younnyeniksoobulrodan extracts 840.0mg/kg 1time/day for 6 weeks, YIB). Rats were sacrificed and TBARS, SOD, CAT, and GSH-px were measured in rat erythrocytes and liver. Results: Plasma and liver TBARS concentrations of YIB group were significantly lower than those of control. Red blood cell(RBC) SOD activities of YIB group was increased(F=3.445, p=0.033, ANOVA test), and RBC catalase activities of all experimental group were not significantly different. RBC GSH-px activities of YIB group was increased(F=9.365,p=0.0001, ANOVA test). Liver SOD activities of YIB group was higher than those of control(F=4.967, p=0.008, ANOVA test). Liver catalase activities of all experimental group were not significantly different, and liver GSH-px activity of YIB group was significantly higher than that of control(F=3.846, p=0.022,ANOVA test). Conclusions: According to the above results, it is considered that Younnyeniksoobulrodan is effective in inhibiting lipid peroxidation and increasing antioxidative enzyme activities in D-galactose induced aging rat.